DOI: 10.1016/s0091-679x(06)79002-1. Cryopreparation Methods for Electron Microscopy of Selected Model Systems

Cryopreparation Methods for Electron Microscopy of Selected Model Systems

10.1016/s0091-679x(06)79002-1

Crossref book-chapter

Elsevier

Methods in Cell Biology (78)

Bibliography

McDonald, K. (2007). Cryopreparation Methods for Electron Microscopy of Selected Model Systems. Cellular Electron Microscopy, 23â56.

Authors 1

Kent McDonald (first)

References 83 Referenced 123

10.1038/sj.emboj.7600366 / EMBO J. / Cryoelectron microscopy of vitreous sections by Al‐Amoudi (2004)
10.1098/rstb.1976.0085 / Philos. Trans. R. Soc. Lond. B Biol. Sci. / The pharynx of C. elegans by Albertson (1976)
10.1002/jemt.1060050305 / J. Electron Microsc. Technol. / Transmission and scanning electron microscopy examination of intracellular organelles in freeze‐substituted Kloeckera and Saccharomyces cerevisiae yeast cells by Baba (1987)
10.1242/dev.119.1.207 / Development / Distribution of tudor protein in the Drosophila embryo suggests separate functions based on site of localization by Bardsley (1993)
10.1093/oxfordjournals.bmb.a071019 / Br. Med. Bull. / The genetics of behaviour by Brenner (1973)
10.1091/mbc.12.6.1645 / Mol. Biol. Cell / Dynamic localization of the Swe1 regulator, Hs17 during the Saccharomyces cervisiae cell cycle by Cid (2001)
10.1083/jcb.120.1.141 / J. Cell Biol. / Three‐dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe by Ding (1993)
10.1016/S0962-8924(00)89071-6 / Trends Cell Biol. / High‐pressure freezing for cryoelectron microscopy by Dubochet (1995)
{'year': '1992', 'series-title': '“Low‐Temperature Microscopy and Analysis.”', 'author': 'Echlin', 'key': '10.1016/S0091-679X(06)79002-1_bib0010'} / “Low‐Temperature Microscopy and Analysis.” by Echlin (1992)
10.1083/jcb.4.5.593 / J. Biophys. Biochem. Cytol. / Methods and principles of fixation by freeze‐substitution by Feder (1958)
10.1111/j.1749-6632.1960.tb49990.x / Ann. NY Acad. Sci. / Low‐temperature preparation techniques for electron microscopy of biological specimens based on rapid freezing with liquid Helium II by Fernandez‐Moran (1960)
10.1016/S0091-679X(01)67003-1 / Methods Cell Biol. / Using rapid freeze and freeze‐substitution for the preparation of yeast cells for electron microscopy and three‐dimensional analysis by Giddings (2001)
10.1002/jemt.1060030206 / J. Electron Microsc. Tech. / Advances in ultrarapid freezing for the preservation of cellular ultrastructure by Gilkey (1986)
10.1083/jcb.151.3.719 / J. Cell Biol. / Prm1p, a pheromone‐regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating by Heiman (2000)
10.1046/j.1365-2818.2003.01237.x / J. Microsc. / On plants and other pets: Practical aspects of freeze substitution and resin embedding by Hess (2003)
10.1006/jsbi.2000.4230 / J. Struct. Biol. / Cryopreparation provides new insight into the effects of Brefeldin A on the structure of the HepG2 golgi apparatus by Hess (2000)
10.1083/jcb.88.3.564 / J. Cell Biol. / Structural changes after transmitter release at the frog Rana pipiens neuro muscular junction by Heuser (1981)
10.1002/jemt.1070240506 / Microsc. Res. Tech. / Impact of freeze substitution on biological electron by Hippe‐Sanwald (1993)
10.1111/j.1365-2818.1994.tb04785.x / J. Microsc. (Oxford) / High‐pressure freezing of cell suspensions in cellulose capillary tubes by Hohenberg (1994)
10.1046/j.1365-2818.1996.820642.x / J. Microsc. / High pressure freezing of tissue obtained by fine needle biopsy by Hohenberg (1996)
10.1111/j.1365-2818.1990.tb02959.x / J. Microsc. (Oxford) / Low‐temperature preparation of chromatin and nuclei by Horowitz (1990)
10.1016/S0022-5320(79)90120-5 / J. Ultrastruct. Res. / Hyphal tip cell ultrastructure of the fungus Fusarium: Improved preservation by freeze‐substitution by Howard (1979)
{'key': '10.1016/S0091-679X(06)79002-1_bib0024', 'series-title': '“The Science of Biological Specimen Preparation 1985”', 'first-page': '175', 'article-title': 'Freeze substitution and low temperature embedding', 'author': 'Humbel', 'year': '1986'} / “The Science of Biological Specimen Preparation 1985” / Freeze substitution and low temperature embedding by Humbel (1986)
10.1177/41.8.8331280 / J. Histochem. Cytochem. / The ultrastructure of the connective tissue matrix of skin and cartilage after high pressure freezing and freeze‐substitution by Keene (1993)
{'key': '10.1016/S0091-679X(06)79002-1_bib0026', 'series-title': '“Cryotechniques in Biological Electron Microscopy”', 'first-page': '35', 'article-title': 'The response of biological macromolecules and supramolecular structures to the physics of specimen cryopreparation', 'author': 'Kellenberger', 'year': '1987'} / “Cryotechniques in Biological Electron Microscopy” / The response of biological macromolecules and supramolecular structures to the physics of specimen cryopreparation by Kellenberger (1987)
{'key': '10.1016/S0091-679X(06)79002-1_bib0027', 'first-page': '265', 'article-title': 'Oogenesis in adult Drosophila melanogaster. IX. Studies on the cytochemistry and ultrastructure of developing oocytes', 'volume': '24', 'author': 'King', 'year': '1960', 'journal-title': 'Growth'} / Growth / Oogenesis in adult Drosophila melanogaster. IX. Studies on the cytochemistry and ultrastructure of developing oocytes by King (1960)
10.1007/BF00334868 / Z. Zellforsch. Mikrosk. Anat. / The comparative submicroscopic morphology of the ring gland of Drosophila melanogaster during the second and third larval instars by King (1966)
10.1016/0248-4900(88)90010-X / Biol. Cell / Secretion systems and membrane‐associated structures in rust fungi after high pressure freezing and freeze‐fracturing by Knauf (1988)
10.1016/0885-5765(89)90076-3 / Physiol. Mol. Plant Pathol. / The haustorial host‐parasite interface in rust‐infected bean leaves after high‐pressure freezing by Knauf (1989)
10.1002/jmor.1051190303 / J. Morphol. / The origin and early differentiation of the egg chamber of Drosophila melanogaster by Koch (1966)
10.1242/dev.01916 / Development / C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes by Kosinski (2005)
10.1046/j.1365-313X.1999.00362.x / Plant J. / High pressure freezing and freeze substitution reveal new aspects of fine structure and maintain protein antigenicity in barley aleurone cells by Lonsdale (1999)
{'key': '10.1016/S0091-679X(06)79002-1_bib0034', 'series-title': '“Microwave Techniques and Protocols”', 'first-page': '139', 'article-title': 'Microwave polymerization in thin layers of LR White allows selection of specimens for immunogold labeling', 'author': 'Lonsdale', 'year': '2001'} / “Microwave Techniques and Protocols” / Microwave polymerization in thin layers of LR White allows selection of specimens for immunogold labeling by Lonsdale (2001)
10.1007/BF01259492 / Chromosoma / Electron‐microscope studies of Drosophila salivary‐gland chromosomes by Lowman (1958)
10.1146/annurev.biochem.73.011303.074112 / Annu. Rev. Biochem. / Structural studies by electron tomography: From cells to molecules by Lucic (2005)
10.1002/jez.1401510302 / J. Exp. Zool. / Fine structure of pole cells and polar granules in Drosophila melanogaster by Mahowald (1962)
10.1016/0012-1606(63)90041-1 / Dev. Biol. / Ultrastructural differentiations during formation of the blastoderm in the Drosophila melanogaster embryo by Mahowald (1963)
{'key': '10.1016/S0091-679X(06)79002-1_bib0039', 'series-title': '“Drosophila melanogaster: Practical Uses in Cell and Molecular Biology”', 'first-page': '411', 'article-title': 'Electron microscopy and EM immunocytochemistry', 'volume': 'Vol. 44', 'author': 'McDonald', 'year': '1994'} / “Drosophila melanogaster: Practical Uses in Cell and Molecular Biology” / Electron microscopy and EM immunocytochemistry by McDonald (1994)
10.1002/jemt.1070240603 / Microsc. Res. Tech. / Improved preservation of ultrastructure in difficult‐to‐fix organisms by high pressure freezing and freeze substitution: I. Drosophila melanogaster and Strongylocentrotus purpuratus embryos by McDonald (1993)
{'key': '10.1016/S0091-679X(06)79002-1_bib0041', 'series-title': '“Guide to Yeast Genetics and Molecular and Cell Biology, Parts B and C”', 'first-page': '96', 'article-title': 'Cryomethods for thin section electron microscopy', 'volume': 'Vol. 351', 'author': 'McDonald', 'year': '2002'} / “Guide to Yeast Genetics and Molecular and Cell Biology, Parts B and C” / Cryomethods for thin section electron microscopy by McDonald (2002)
{'key': '10.1016/S0091-679X(06)79002-1_bib0042', 'series-title': '“Electron Microscopy Methods and Protocols”', 'first-page': '77', 'article-title': 'High pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling', 'volume': 'Vol. 117', 'author': 'McDonald', 'year': '1999'} / “Electron Microscopy Methods and Protocols” / High pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling by McDonald (1999)
{'key': '10.1016/S0091-679X(06)79002-1_bib0043', 'series-title': '“Electron Microscopy Methods and Protocols”', 'first-page': '143', 'article-title': 'Recent advances in high pressure freezing: Equipment and specimen loading methods', 'volume': 'Vol. 369', 'author': 'McDonald', 'year': '2007'} / “Electron Microscopy Methods and Protocols” / Recent advances in high pressure freezing: Equipment and specimen loading methods by McDonald (2007)
{'key': '10.1016/S0091-679X(06)79002-1_bib0044', 'series-title': '“Drosophila: A Laboratory Manual”', 'first-page': '245', 'article-title': 'Preparing thin sections of Drosophila for examination in the transmission electron microscope', 'author': 'McDonald', 'year': '2000'} / “Drosophila: A Laboratory Manual” / Preparing thin sections of Drosophila for examination in the transmission electron microscope by McDonald (2000)
10.1016/j.tcb.2004.11.009 / Trends Cell Biol. / New views of cells in 3‐D: An introduction to electron tomography by McIntosh (2005)
{'key': '10.1016/S0091-679X(06)79002-1_bib0046', 'first-page': '111', 'article-title': 'Plastic embedding mixtures for use in electron microscopy', 'volume': '39', 'author': 'Mollenhauer', 'year': '1964', 'journal-title': 'Stain Technol.'} / Stain Technol. / Plastic embedding mixtures for use in electron microscopy by Mollenhauer (1964)
10.1007/BF00408697 / Arch. Mikrobiol. / Endoplasmic reticulum as the initiator of bud formation in yeast by Moor (1967)
{'key': '10.1016/S0091-679X(06)79002-1_bib0048', 'series-title': '“Cryotechniques in Biological Electron Microscopy”', 'first-page': '175', 'article-title': 'Theory and practice of high pressure freezing', 'author': 'Moor', 'year': '1987'} / “Cryotechniques in Biological Electron Microscopy” / Theory and practice of high pressure freezing by Moor (1987)
10.1083/jcb.17.3.609 / J. Cell Biol. / Fine structure in frozen‐etched yeast cells by Moor (1963)
{'key': '10.1016/S0091-679X(06)79002-1_bib0050', 'first-page': '33', 'article-title': 'Snap‐freezing under high pressure: A new fixation technique for freeze‐etching', 'volume': 'Vol. 2', 'author': 'Moor', 'year': '1968'} / Snap‐freezing under high pressure: A new fixation technique for freeze‐etching by Moor (1968)
{'key': '10.1016/S0091-679X(06)79002-1_bib0051', 'first-page': '720', 'article-title': 'Improved structural preservation by freeze substitution', 'volume': 'Vol. 2', 'author': 'Müller', 'year': '1980'} / Improved structural preservation by freeze substitution by Müller (1980)
{'key': '10.1016/S0091-679X(06)79002-1_bib0052', 'first-page': '131', 'article-title': 'Cryofixation of thick specimens by high‐pressure freezing', 'author': 'Müller', 'year': '1984'} / Cryofixation of thick specimens by high‐pressure freezing by Müller (1984)
10.1046/j.1365-2818.2003.01250.x / J. Microsc. (Oxford) / Cryoimmobilization and three‐dimensional visualization of C. elegans ultrastructure by Müller‐Reichert (2003)
10.1007/s00412-002-0220-6 / Chromosoma / Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3‐D modeling of mitotic spindles by Müller‐Reichert (2003)
10.1046/j.1365-2818.2003.01238.x / J. Microsc. / Influence of aldehyde fixation on the morphology of endosomes and lysosomes: Quantitative analysis and electron tomography by Murk (2003)
10.1002/jemt.1070240604 / Microsc. Res. Tech. / Freeze sustitution after fast‐freeze fixation in preparation for immunocytochemistry by Nicolas (1993)
10.1083/jcb.152.1.F1 / J. Cell Biol. / Molecular machines: Putting the pieces together by Nogales (2001)
{'key': '10.1016/S0091-679X(06)79002-1_bib0058', 'series-title': '“Guide to Yeast Genetics and Molecular and Cell Biology, Parts B and C”', 'first-page': '81', 'article-title': 'Electron tomography of yeast cells', 'volume': 'Vol. 351', 'author': "O'Toole", 'year': '2002'} / “Guide to Yeast Genetics and Molecular and Cell Biology, Parts B and C” / Electron tomography of yeast cells by O'Toole (2002)
10.1083/jcb.200304035 / J. Cell Biol. / Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans by O'Toole (2003)
10.1111/j.1365-2818.2004.01260.x / J. Microsc. (Oxford) / High‐pressure freezing of epithelial cells on sapphire coverslips by Reipert (2004)
10.1111/j.0906-6705.2004.00180.x / Exp. Dermatol. / High pressure immobilization of murine skin reveals novel structural features and prevents extraction artifacts by Reipert (2004)
10.1083/jcb.17.1.208 / J. Cell Biol. / The use of lead citrate at high pH as an electron‐opaque stain in electron microscopy by Reynolds (1963)
10.1002/jcp.1030210206 / J. Cell. Comp. Physiol. / Electron microscope studies of squid giant nerve axoplasm by Richards (1943)
{'key': '10.1016/S0091-679X(06)79002-1_bib0064', 'article-title': 'Low temperature methods in biological electron microscopy', 'volume': 'Vol. 10', 'author': 'Robards', 'year': '1985'} / Low temperature methods in biological electron microscopy by Robards (1985)
10.1111/j.1365-2818.1993.tb03393.x / J. Microsc. (Oxford) / Vitrification depth can be increased more than 10‐fold by high‐pressure freezing by Sartori (1993)
10.1083/jcb.200303192 / J. Cell Biol. / Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in Drosophila by Schulte (2003)
10.1046/j.1365-2818.1998.00389.x / J. Microsc. (Oxford) / On optimizing high‐pressure freezing: From heat transfer theory to a new microbiopsy device by Shimoni (1998)
10.1111/j.1365-2818.1982.tb00336.x / J. Microsc. (Oxford) / Experiments on freezing damage with freeze substitution using moth antennae as test objects by Steinbrecht (1982)
10.1111/j.1365-2818.1985.tb02658.x / J. Microsc. (Oxford) / Recrystallization and ice‐crystal growth in a biological specimen, as shown by a simple freeze substitution method by Steinbrecht (1985)
10.1002/jemt.1070240605 / Microsc. Res. Tech. / Freeze substitution for morphological and immunocytochemical studies in insects by Steinbrecht (1993)
{'key': '10.1016/S0091-679X(06)79002-1_bib0071', 'series-title': '“Cryotechniques in Biological Electron Microscopy”', 'first-page': '149', 'article-title': 'Freeze substitution and freeze drying', 'author': 'Steinbrecht', 'year': '1987'} / “Cryotechniques in Biological Electron Microscopy” / Freeze substitution and freeze drying by Steinbrecht (1987)
10.1046/j.1365-2818.2001.00919.x / J. Microsc. (Oxford) / A new approach for cryofixation by high‐pressure freezing by Studer (2001)
10.1111/j.1365-2818.1995.tb03648.x / J. Microsc. (Oxford) / Vitrification of articular cartilage by high‐pressure freezing by Studer (1995)
{'key': '10.1016/S0091-679X(06)79002-1_bib0074', 'series-title': '“The Nematode Caenorhabditis elegans”', 'first-page': '587', 'article-title': 'Methods', 'author': 'Sulston', 'year': '1988'} / “The Nematode Caenorhabditis elegans” / Methods by Sulston (1988)
10.1242/jcs.80.1.253 / J. Cell Sci. / Mitosis in the fission yeast Schizosacchoromyces pombe as revealed by freeze substitution electron microscopy by Tanaka (1986)
10.1046/j.1365-2818.1998.00385.x / J. Microsc. (Oxford) / Heptane and isooctane as embedding fluids for high‐pressure freezing of Petunia ovules followed by freeze‐substitution by Thijssen (1998)
10.1002/ar.1091490307 / Anat. Rec. / Electron microscopy after rapid freezing on a metal surface and substitution fixation by Van Harreveld (1964)
10.1046/j.1365-2818.2003.01236.x / J. Microsc. (Oxford) / Recent progress in freeze‐fracturing of high‐pressure frozen samples by Walther (2003)
10.1046/j.1365-2818.2002.01064.x / J. Microsc. (Oxford) / Freeze substitution of high‐pressure frozen samples: The visibility of biological membranes is improved when the substitution medium contains water by Walther (2002)
10.1002/cne.901600305 / J. Comp. Neurol. / Electron microscopical reconstruction of the anterior sensory anatomy of the nematode Caenorhabditis elegans by Ward (1975)
10.1007/BF01403336 / Protoplasma / The hyphae of Uromyces appendiculatus within the leaf tissue after high pressure freezing and freeze substitution by Welter (1988)
10.1098/rstb.1976.0086 / Philos. Trans. R. Soc. Lond. B Biol. Sci. / The structure of the ventral nerve cord of C. elegans by White (1976)
10.1083/jcb.129.6.1601 / J. Cell Biol. / Three‐dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle by Winey (1995)
10.1111/j.0022-2720.2004.01395.x / J. Microsc. (Oxford) / Direct visualization of receptor arrays in frozen‐hydrated sections and plunge‐frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr by Zhang (2004)

Dates

Type	When
Created	18 years, 5 months ago (Feb. 26, 2007, 1:58 p.m.)
Deposited	4 years ago (Aug. 9, 2021, 12:36 a.m.)
Indexed	3 weeks, 2 days ago (Aug. 2, 2025, 1:22 a.m.)
Issued	18 years, 7 months ago (Jan. 1, 2007)
Published	18 years, 7 months ago (Jan. 1, 2007)
Published Print	18 years, 7 months ago (Jan. 1, 2007)

Funders 0

None

BibTeX

@inbook{McDonald_2007, title={Cryopreparation Methods for Electron Microscopy of Selected Model Systems}, ISBN={9780123706478}, ISSN={0091-679X}, url={http://dx.doi.org/10.1016/s0091-679x(06)79002-1}, DOI={10.1016/s0091-679x(06)79002-1}, booktitle={Cellular Electron Microscopy}, publisher={Elsevier}, author={McDonald, Kent}, year={2007}, pages={23–56} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T05:22:17Z",
    "timestamp": 1754112137528
  },
  "reference-count": 83,
  "publisher": "Elsevier",
  "isbn-type": [
    {
      "type": "print",
      "value": "9780123706478"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2007
      ]
    ]
  },
  "DOI": "10.1016/s0091-679x(06)79002-1",
  "type": "book-chapter",
  "created": {
    "date-parts": [
      [
        2007,
        2,
        26
      ]
    ],
    "date-time": "2007-02-26T18:58:26Z",
    "timestamp": 1172516306000
  },
  "page": "23-56",
  "source": "Crossref",
  "is-referenced-by-count": 123,
  "title": "Cryopreparation Methods for Electron Microscopy of Selected Model Systems",
  "prefix": "10.1016",
  "author": [
    {
      "given": "Kent",
      "family": "McDonald",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0001",
      "doi-asserted-by": "crossref",
      "first-page": "3583",
      "DOI": "10.1038/sj.emboj.7600366",
      "article-title": "Cryoelectron microscopy of vitreous sections",
      "volume": "23",
      "author": "Al\u2010Amoudi",
      "year": "2004",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0002",
      "doi-asserted-by": "crossref",
      "first-page": "299",
      "DOI": "10.1098/rstb.1976.0085",
      "article-title": "The pharynx of C. elegans",
      "volume": "275",
      "author": "Albertson",
      "year": "1976",
      "journal-title": "Philos. Trans. R. Soc. Lond. B Biol. Sci."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0003",
      "doi-asserted-by": "crossref",
      "first-page": "249",
      "DOI": "10.1002/jemt.1060050305",
      "article-title": "Transmission and scanning electron microscopy examination of intracellular organelles in freeze\u2010substituted Kloeckera and Saccharomyces cerevisiae yeast cells",
      "volume": "5",
      "author": "Baba",
      "year": "1987",
      "journal-title": "J. Electron Microsc. Technol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0004",
      "doi-asserted-by": "crossref",
      "first-page": "207",
      "DOI": "10.1242/dev.119.1.207",
      "article-title": "Distribution of tudor protein in the Drosophila embryo suggests separate functions based on site of localization",
      "volume": "119",
      "author": "Bardsley",
      "year": "1993",
      "journal-title": "Development"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0005",
      "doi-asserted-by": "crossref",
      "first-page": "269",
      "DOI": "10.1093/oxfordjournals.bmb.a071019",
      "article-title": "The genetics of behaviour",
      "volume": "29",
      "author": "Brenner",
      "year": "1973",
      "journal-title": "Br. Med. Bull."
    },
    {
      "issue": "6",
      "key": "10.1016/S0091-679X(06)79002-1_bib0007",
      "doi-asserted-by": "crossref",
      "first-page": "1654",
      "DOI": "10.1091/mbc.12.6.1645",
      "article-title": "Dynamic localization of the Swe1 regulator, Hs17 during the Saccharomyces cervisiae cell cycle",
      "volume": "12",
      "author": "Cid",
      "year": "2001",
      "journal-title": "Mol. Biol. Cell"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0008",
      "doi-asserted-by": "crossref",
      "first-page": "141",
      "DOI": "10.1083/jcb.120.1.141",
      "article-title": "Three\u2010dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe",
      "volume": "120",
      "author": "Ding",
      "year": "1993",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0009",
      "doi-asserted-by": "crossref",
      "first-page": "366",
      "DOI": "10.1016/S0962-8924(00)89071-6",
      "article-title": "High\u2010pressure freezing for cryoelectron microscopy",
      "volume": "5",
      "author": "Dubochet",
      "year": "1995",
      "journal-title": "Trends Cell Biol."
    },
    {
      "year": "1992",
      "series-title": "\u201cLow\u2010Temperature Microscopy and Analysis.\u201d",
      "author": "Echlin",
      "key": "10.1016/S0091-679X(06)79002-1_bib0010"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0011",
      "doi-asserted-by": "crossref",
      "first-page": "593",
      "DOI": "10.1083/jcb.4.5.593",
      "article-title": "Methods and principles of fixation by freeze\u2010substitution",
      "volume": "4",
      "author": "Feder",
      "year": "1958",
      "journal-title": "J. Biophys. Biochem. Cytol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0012",
      "doi-asserted-by": "crossref",
      "first-page": "689",
      "DOI": "10.1111/j.1749-6632.1960.tb49990.x",
      "article-title": "Low\u2010temperature preparation techniques for electron microscopy of biological specimens based on rapid freezing with liquid Helium II",
      "volume": "85",
      "author": "Fernandez\u2010Moran",
      "year": "1960",
      "journal-title": "Ann. NY Acad. Sci."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0013",
      "doi-asserted-by": "crossref",
      "first-page": "27",
      "DOI": "10.1016/S0091-679X(01)67003-1",
      "article-title": "Using rapid freeze and freeze\u2010substitution for the preparation of yeast cells for electron microscopy and three\u2010dimensional analysis",
      "volume": "67",
      "author": "Giddings",
      "year": "2001",
      "journal-title": "Methods Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0014",
      "doi-asserted-by": "crossref",
      "first-page": "177",
      "DOI": "10.1002/jemt.1060030206",
      "article-title": "Advances in ultrarapid freezing for the preservation of cellular ultrastructure",
      "volume": "3",
      "author": "Gilkey",
      "year": "1986",
      "journal-title": "J. Electron Microsc. Tech."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0015",
      "doi-asserted-by": "crossref",
      "first-page": "719",
      "DOI": "10.1083/jcb.151.3.719",
      "article-title": "Prm1p, a pheromone\u2010regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating",
      "volume": "151",
      "author": "Heiman",
      "year": "2000",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0016",
      "doi-asserted-by": "crossref",
      "first-page": "44",
      "DOI": "10.1046/j.1365-2818.2003.01237.x",
      "article-title": "On plants and other pets: Practical aspects of freeze substitution and resin embedding",
      "volume": "212",
      "author": "Hess",
      "year": "2003",
      "journal-title": "J. Microsc."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0017",
      "doi-asserted-by": "crossref",
      "first-page": "63",
      "DOI": "10.1006/jsbi.2000.4230",
      "article-title": "Cryopreparation provides new insight into the effects of Brefeldin A on the structure of the HepG2 golgi apparatus",
      "volume": "130",
      "author": "Hess",
      "year": "2000",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0018",
      "doi-asserted-by": "crossref",
      "first-page": "564",
      "DOI": "10.1083/jcb.88.3.564",
      "article-title": "Structural changes after transmitter release at the frog Rana pipiens neuro muscular junction",
      "volume": "88",
      "author": "Heuser",
      "year": "1981",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0019",
      "doi-asserted-by": "crossref",
      "first-page": "400",
      "DOI": "10.1002/jemt.1070240506",
      "article-title": "Impact of freeze substitution on biological electron",
      "volume": "24",
      "author": "Hippe\u2010Sanwald",
      "year": "1993",
      "journal-title": "Microsc. Res. Tech."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0020",
      "doi-asserted-by": "crossref",
      "first-page": "34",
      "DOI": "10.1111/j.1365-2818.1994.tb04785.x",
      "article-title": "High\u2010pressure freezing of cell suspensions in cellulose capillary tubes",
      "volume": "175",
      "author": "Hohenberg",
      "year": "1994",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0021",
      "doi-asserted-by": "crossref",
      "first-page": "133",
      "DOI": "10.1046/j.1365-2818.1996.820642.x",
      "article-title": "High pressure freezing of tissue obtained by fine needle biopsy",
      "volume": "183",
      "author": "Hohenberg",
      "year": "1996",
      "journal-title": "J. Microsc."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0022",
      "doi-asserted-by": "crossref",
      "first-page": "205",
      "DOI": "10.1111/j.1365-2818.1990.tb02959.x",
      "article-title": "Low\u2010temperature preparation of chromatin and nuclei",
      "volume": "157",
      "author": "Horowitz",
      "year": "1990",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0023",
      "doi-asserted-by": "crossref",
      "first-page": "224",
      "DOI": "10.1016/S0022-5320(79)90120-5",
      "article-title": "Hyphal tip cell ultrastructure of the fungus Fusarium: Improved preservation by freeze\u2010substitution",
      "volume": "66",
      "author": "Howard",
      "year": "1979",
      "journal-title": "J. Ultrastruct. Res."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0024",
      "series-title": "\u201cThe Science of Biological Specimen Preparation 1985\u201d",
      "first-page": "175",
      "article-title": "Freeze substitution and low temperature embedding",
      "author": "Humbel",
      "year": "1986"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0025",
      "doi-asserted-by": "crossref",
      "first-page": "1141",
      "DOI": "10.1177/41.8.8331280",
      "article-title": "The ultrastructure of the connective tissue matrix of skin and cartilage after high pressure freezing and freeze\u2010substitution",
      "volume": "41",
      "author": "Keene",
      "year": "1993",
      "journal-title": "J. Histochem. Cytochem."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0026",
      "series-title": "\u201cCryotechniques in Biological Electron Microscopy\u201d",
      "first-page": "35",
      "article-title": "The response of biological macromolecules and supramolecular structures to the physics of specimen cryopreparation",
      "author": "Kellenberger",
      "year": "1987"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0027",
      "first-page": "265",
      "article-title": "Oogenesis in adult Drosophila melanogaster. IX. Studies on the cytochemistry and ultrastructure of developing oocytes",
      "volume": "24",
      "author": "King",
      "year": "1960",
      "journal-title": "Growth"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0028",
      "doi-asserted-by": "crossref",
      "first-page": "272",
      "DOI": "10.1007/BF00334868",
      "article-title": "The comparative submicroscopic morphology of the ring gland of Drosophila melanogaster during the second and third larval instars",
      "volume": "73",
      "author": "King",
      "year": "1966",
      "journal-title": "Z. Zellforsch. Mikrosk. Anat."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0029",
      "doi-asserted-by": "crossref",
      "first-page": "363",
      "DOI": "10.1016/0248-4900(88)90010-X",
      "article-title": "Secretion systems and membrane\u2010associated structures in rust fungi after high pressure freezing and freeze\u2010fracturing",
      "volume": "64",
      "author": "Knauf",
      "year": "1988",
      "journal-title": "Biol. Cell"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0030",
      "doi-asserted-by": "crossref",
      "first-page": "519",
      "DOI": "10.1016/0885-5765(89)90076-3",
      "article-title": "The haustorial host\u2010parasite interface in rust\u2010infected bean leaves after high\u2010pressure freezing",
      "volume": "34",
      "author": "Knauf",
      "year": "1989",
      "journal-title": "Physiol. Mol. Plant Pathol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0031",
      "doi-asserted-by": "crossref",
      "first-page": "283",
      "DOI": "10.1002/jmor.1051190303",
      "article-title": "The origin and early differentiation of the egg chamber of Drosophila melanogaster",
      "volume": "119",
      "author": "Koch",
      "year": "1966",
      "journal-title": "J. Morphol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0032",
      "doi-asserted-by": "crossref",
      "first-page": "3357",
      "DOI": "10.1242/dev.01916",
      "article-title": "C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes",
      "volume": "132",
      "author": "Kosinski",
      "year": "2005",
      "journal-title": "Development"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0033",
      "doi-asserted-by": "crossref",
      "first-page": "221",
      "DOI": "10.1046/j.1365-313X.1999.00362.x",
      "article-title": "High pressure freezing and freeze substitution reveal new aspects of fine structure and maintain protein antigenicity in barley aleurone cells",
      "volume": "17",
      "author": "Lonsdale",
      "year": "1999",
      "journal-title": "Plant J."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0034",
      "series-title": "\u201cMicrowave Techniques and Protocols\u201d",
      "first-page": "139",
      "article-title": "Microwave polymerization in thin layers of LR White allows selection of specimens for immunogold labeling",
      "author": "Lonsdale",
      "year": "2001"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0035",
      "doi-asserted-by": "crossref",
      "first-page": "30",
      "DOI": "10.1007/BF01259492",
      "article-title": "Electron\u2010microscope studies of Drosophila salivary\u2010gland chromosomes",
      "volume": "8",
      "author": "Lowman",
      "year": "1958",
      "journal-title": "Chromosoma"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0036",
      "doi-asserted-by": "crossref",
      "first-page": "833",
      "DOI": "10.1146/annurev.biochem.73.011303.074112",
      "article-title": "Structural studies by electron tomography: From cells to molecules",
      "volume": "74",
      "author": "Lucic",
      "year": "2005",
      "journal-title": "Annu. Rev. Biochem."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0037",
      "doi-asserted-by": "crossref",
      "first-page": "201",
      "DOI": "10.1002/jez.1401510302",
      "article-title": "Fine structure of pole cells and polar granules in Drosophila melanogaster",
      "volume": "3",
      "author": "Mahowald",
      "year": "1962",
      "journal-title": "J. Exp. Zool."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0038",
      "doi-asserted-by": "crossref",
      "first-page": "186",
      "DOI": "10.1016/0012-1606(63)90041-1",
      "article-title": "Ultrastructural differentiations during formation of the blastoderm in the Drosophila melanogaster embryo",
      "volume": "1963",
      "author": "Mahowald",
      "year": "1963",
      "journal-title": "Dev. Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0039",
      "series-title": "\u201cDrosophila melanogaster: Practical Uses in Cell and Molecular Biology\u201d",
      "first-page": "411",
      "article-title": "Electron microscopy and EM immunocytochemistry",
      "volume": "Vol. 44",
      "author": "McDonald",
      "year": "1994"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0040",
      "doi-asserted-by": "crossref",
      "first-page": "465",
      "DOI": "10.1002/jemt.1070240603",
      "article-title": "Improved preservation of ultrastructure in difficult\u2010to\u2010fix organisms by high pressure freezing and freeze substitution: I. Drosophila melanogaster and Strongylocentrotus purpuratus embryos",
      "volume": "24",
      "author": "McDonald",
      "year": "1993",
      "journal-title": "Microsc. Res. Tech."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0041",
      "series-title": "\u201cGuide to Yeast Genetics and Molecular and Cell Biology, Parts B and C\u201d",
      "first-page": "96",
      "article-title": "Cryomethods for thin section electron microscopy",
      "volume": "Vol. 351",
      "author": "McDonald",
      "year": "2002"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0042",
      "series-title": "\u201cElectron Microscopy Methods and Protocols\u201d",
      "first-page": "77",
      "article-title": "High pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling",
      "volume": "Vol. 117",
      "author": "McDonald",
      "year": "1999"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0043",
      "series-title": "\u201cElectron Microscopy Methods and Protocols\u201d",
      "first-page": "143",
      "article-title": "Recent advances in high pressure freezing: Equipment and specimen loading methods",
      "volume": "Vol. 369",
      "author": "McDonald",
      "year": "2007"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0044",
      "series-title": "\u201cDrosophila: A Laboratory Manual\u201d",
      "first-page": "245",
      "article-title": "Preparing thin sections of Drosophila for examination in the transmission electron microscope",
      "author": "McDonald",
      "year": "2000"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0045",
      "doi-asserted-by": "crossref",
      "first-page": "43",
      "DOI": "10.1016/j.tcb.2004.11.009",
      "article-title": "New views of cells in 3\u2010D: An introduction to electron tomography",
      "volume": "15",
      "author": "McIntosh",
      "year": "2005",
      "journal-title": "Trends Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0046",
      "first-page": "111",
      "article-title": "Plastic embedding mixtures for use in electron microscopy",
      "volume": "39",
      "author": "Mollenhauer",
      "year": "1964",
      "journal-title": "Stain Technol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0047",
      "doi-asserted-by": "crossref",
      "first-page": "135",
      "DOI": "10.1007/BF00408697",
      "article-title": "Endoplasmic reticulum as the initiator of bud formation in yeast",
      "volume": "57",
      "author": "Moor",
      "year": "1967",
      "journal-title": "Arch. Mikrobiol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0048",
      "series-title": "\u201cCryotechniques in Biological Electron Microscopy\u201d",
      "first-page": "175",
      "article-title": "Theory and practice of high pressure freezing",
      "author": "Moor",
      "year": "1987"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0049",
      "doi-asserted-by": "crossref",
      "first-page": "609",
      "DOI": "10.1083/jcb.17.3.609",
      "article-title": "Fine structure in frozen\u2010etched yeast cells",
      "volume": "17",
      "author": "Moor",
      "year": "1963",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0050",
      "first-page": "33",
      "article-title": "Snap\u2010freezing under high pressure: A new fixation technique for freeze\u2010etching",
      "volume": "Vol. 2",
      "author": "Moor",
      "year": "1968"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0051",
      "first-page": "720",
      "article-title": "Improved structural preservation by freeze substitution",
      "volume": "Vol. 2",
      "author": "M\u00fcller",
      "year": "1980"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0052",
      "first-page": "131",
      "article-title": "Cryofixation of thick specimens by high\u2010pressure freezing",
      "author": "M\u00fcller",
      "year": "1984"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0053",
      "doi-asserted-by": "crossref",
      "first-page": "71",
      "DOI": "10.1046/j.1365-2818.2003.01250.x",
      "article-title": "Cryoimmobilization and three\u2010dimensional visualization of C. elegans ultrastructure",
      "volume": "212",
      "author": "M\u00fcller\u2010Reichert",
      "year": "2003",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0054",
      "doi-asserted-by": "crossref",
      "first-page": "417",
      "DOI": "10.1007/s00412-002-0220-6",
      "article-title": "Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3\u2010D modeling of mitotic spindles",
      "volume": "111",
      "author": "M\u00fcller\u2010Reichert",
      "year": "2003",
      "journal-title": "Chromosoma"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0055",
      "doi-asserted-by": "crossref",
      "first-page": "81",
      "DOI": "10.1046/j.1365-2818.2003.01238.x",
      "article-title": "Influence of aldehyde fixation on the morphology of endosomes and lysosomes: Quantitative analysis and electron tomography",
      "volume": "212",
      "author": "Murk",
      "year": "2003",
      "journal-title": "J. Microsc."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0056",
      "doi-asserted-by": "crossref",
      "first-page": "474",
      "DOI": "10.1002/jemt.1070240604",
      "article-title": "Freeze sustitution after fast\u2010freeze fixation in preparation for immunocytochemistry",
      "volume": "24",
      "author": "Nicolas",
      "year": "1993",
      "journal-title": "Microsc. Res. Tech."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0057",
      "doi-asserted-by": "crossref",
      "first-page": "F1",
      "DOI": "10.1083/jcb.152.1.F1",
      "article-title": "Molecular machines: Putting the pieces together",
      "volume": "152",
      "author": "Nogales",
      "year": "2001",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0058",
      "series-title": "\u201cGuide to Yeast Genetics and Molecular and Cell Biology, Parts B and C\u201d",
      "first-page": "81",
      "article-title": "Electron tomography of yeast cells",
      "volume": "Vol. 351",
      "author": "O'Toole",
      "year": "2002"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0059",
      "doi-asserted-by": "crossref",
      "first-page": "451",
      "DOI": "10.1083/jcb.200304035",
      "article-title": "Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans",
      "volume": "163",
      "author": "O'Toole",
      "year": "2003",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0060",
      "doi-asserted-by": "crossref",
      "first-page": "81",
      "DOI": "10.1111/j.1365-2818.2004.01260.x",
      "article-title": "High\u2010pressure freezing of epithelial cells on sapphire coverslips",
      "volume": "213",
      "author": "Reipert",
      "year": "2004",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0061",
      "doi-asserted-by": "crossref",
      "first-page": "419",
      "DOI": "10.1111/j.0906-6705.2004.00180.x",
      "article-title": "High pressure immobilization of murine skin reveals novel structural features and prevents extraction artifacts",
      "volume": "13",
      "author": "Reipert",
      "year": "2004",
      "journal-title": "Exp. Dermatol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0062",
      "doi-asserted-by": "crossref",
      "first-page": "208",
      "DOI": "10.1083/jcb.17.1.208",
      "article-title": "The use of lead citrate at high pH as an electron\u2010opaque stain in electron microscopy",
      "volume": "17",
      "author": "Reynolds",
      "year": "1963",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0063",
      "doi-asserted-by": "crossref",
      "first-page": "129",
      "DOI": "10.1002/jcp.1030210206",
      "article-title": "Electron microscope studies of squid giant nerve axoplasm",
      "volume": "21",
      "author": "Richards",
      "year": "1943",
      "journal-title": "J. Cell. Comp. Physiol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0064",
      "article-title": "Low temperature methods in biological electron microscopy",
      "volume": "Vol. 10",
      "author": "Robards",
      "year": "1985"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0065",
      "doi-asserted-by": "crossref",
      "first-page": "55",
      "DOI": "10.1111/j.1365-2818.1993.tb03393.x",
      "article-title": "Vitrification depth can be increased more than 10\u2010fold by high\u2010pressure freezing",
      "volume": "172",
      "author": "Sartori",
      "year": "1993",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0066",
      "doi-asserted-by": "crossref",
      "first-page": "991",
      "DOI": "10.1083/jcb.200303192",
      "article-title": "Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in Drosophila",
      "volume": "161",
      "author": "Schulte",
      "year": "2003",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0067",
      "doi-asserted-by": "crossref",
      "first-page": "236",
      "DOI": "10.1046/j.1365-2818.1998.00389.x",
      "article-title": "On optimizing high\u2010pressure freezing: From heat transfer theory to a new microbiopsy device",
      "volume": "192",
      "author": "Shimoni",
      "year": "1998",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0068",
      "doi-asserted-by": "crossref",
      "first-page": "187",
      "DOI": "10.1111/j.1365-2818.1982.tb00336.x",
      "article-title": "Experiments on freezing damage with freeze substitution using moth antennae as test objects",
      "volume": "125",
      "author": "Steinbrecht",
      "year": "1982",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0069",
      "doi-asserted-by": "crossref",
      "first-page": "41",
      "DOI": "10.1111/j.1365-2818.1985.tb02658.x",
      "article-title": "Recrystallization and ice\u2010crystal growth in a biological specimen, as shown by a simple freeze substitution method",
      "volume": "140",
      "author": "Steinbrecht",
      "year": "1985",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0070",
      "doi-asserted-by": "crossref",
      "first-page": "488",
      "DOI": "10.1002/jemt.1070240605",
      "article-title": "Freeze substitution for morphological and immunocytochemical studies in insects",
      "volume": "24",
      "author": "Steinbrecht",
      "year": "1993",
      "journal-title": "Microsc. Res. Tech."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0071",
      "series-title": "\u201cCryotechniques in Biological Electron Microscopy\u201d",
      "first-page": "149",
      "article-title": "Freeze substitution and freeze drying",
      "author": "Steinbrecht",
      "year": "1987"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0072",
      "doi-asserted-by": "crossref",
      "first-page": "285",
      "DOI": "10.1046/j.1365-2818.2001.00919.x",
      "article-title": "A new approach for cryofixation by high\u2010pressure freezing",
      "volume": "203",
      "author": "Studer",
      "year": "2001",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0073",
      "doi-asserted-by": "crossref",
      "first-page": "321",
      "DOI": "10.1111/j.1365-2818.1995.tb03648.x",
      "article-title": "Vitrification of articular cartilage by high\u2010pressure freezing",
      "volume": "179",
      "author": "Studer",
      "year": "1995",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0074",
      "series-title": "\u201cThe Nematode Caenorhabditis elegans\u201d",
      "first-page": "587",
      "article-title": "Methods",
      "author": "Sulston",
      "year": "1988"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0075",
      "doi-asserted-by": "crossref",
      "first-page": "253",
      "DOI": "10.1242/jcs.80.1.253",
      "article-title": "Mitosis in the fission yeast Schizosacchoromyces pombe as revealed by freeze substitution electron microscopy",
      "volume": "80",
      "author": "Tanaka",
      "year": "1986",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0076",
      "doi-asserted-by": "crossref",
      "first-page": "228",
      "DOI": "10.1046/j.1365-2818.1998.00385.x",
      "article-title": "Heptane and isooctane as embedding fluids for high\u2010pressure freezing of Petunia ovules followed by freeze\u2010substitution",
      "volume": "192",
      "author": "Thijssen",
      "year": "1998",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0077",
      "doi-asserted-by": "crossref",
      "first-page": "381",
      "DOI": "10.1002/ar.1091490307",
      "article-title": "Electron microscopy after rapid freezing on a metal surface and substitution fixation",
      "volume": "149",
      "author": "Van Harreveld",
      "year": "1964",
      "journal-title": "Anat. Rec."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0078",
      "doi-asserted-by": "crossref",
      "first-page": "34",
      "DOI": "10.1046/j.1365-2818.2003.01236.x",
      "article-title": "Recent progress in freeze\u2010fracturing of high\u2010pressure frozen samples",
      "volume": "212",
      "author": "Walther",
      "year": "2003",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0079",
      "doi-asserted-by": "crossref",
      "first-page": "3",
      "DOI": "10.1046/j.1365-2818.2002.01064.x",
      "article-title": "Freeze substitution of high\u2010pressure frozen samples: The visibility of biological membranes is improved when the substitution medium contains water",
      "volume": "208",
      "author": "Walther",
      "year": "2002",
      "journal-title": "J. Microsc. (Oxford)"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0080",
      "doi-asserted-by": "crossref",
      "first-page": "313",
      "DOI": "10.1002/cne.901600305",
      "article-title": "Electron microscopical reconstruction of the anterior sensory anatomy of the nematode Caenorhabditis elegans",
      "volume": "160",
      "author": "Ward",
      "year": "1975",
      "journal-title": "J. Comp. Neurol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0081",
      "doi-asserted-by": "crossref",
      "first-page": "91",
      "DOI": "10.1007/BF01403336",
      "article-title": "The hyphae of Uromyces appendiculatus within the leaf tissue after high pressure freezing and freeze substitution",
      "volume": "147",
      "author": "Welter",
      "year": "1988",
      "journal-title": "Protoplasma"
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0082",
      "doi-asserted-by": "crossref",
      "first-page": "327",
      "DOI": "10.1098/rstb.1976.0086",
      "article-title": "The structure of the ventral nerve cord of C. elegans",
      "volume": "275",
      "author": "White",
      "year": "1976",
      "journal-title": "Philos. Trans. R. Soc. Lond. B Biol. Sci."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0083",
      "doi-asserted-by": "crossref",
      "first-page": "1601",
      "DOI": "10.1083/jcb.129.6.1601",
      "article-title": "Three\u2010dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle",
      "volume": "129",
      "author": "Winey",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(06)79002-1_bib0084",
      "doi-asserted-by": "crossref",
      "first-page": "76",
      "DOI": "10.1111/j.0022-2720.2004.01395.x",
      "article-title": "Direct visualization of receptor arrays in frozen\u2010hydrated sections and plunge\u2010frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr",
      "volume": "216",
      "author": "Zhang",
      "year": "2004",
      "journal-title": "J. Microsc. (Oxford)"
    }
  ],
  "container-title": "Methods in Cell Biology",
  "original-title": [],
  "language": "en",
  "deposited": {
    "date-parts": [
      [
        2021,
        8,
        9
      ]
    ],
    "date-time": "2021-08-09T04:36:25Z",
    "timestamp": 1628483785000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S0091679X06790021"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2007
      ]
    ]
  },
  "ISBN": [
    "9780123706478"
  ],
  "references-count": 83,
  "URL": "http://dx.doi.org/10.1016/S0091-679X(06)79002-1",
  "relation": {},
  "ISSN": [
    "0091-679X"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2007
      ]
    ]
  }
}