DOI: 10.1016/s0091-679x(01)65011-8. Chapter 10 Analysis and prediction of mitochondrial targeting peptides

Chapter 10 Analysis and prediction of mitochondrial targeting peptides

10.1016/s0091-679x(01)65011-8

Crossref book-chapter

Elsevier

Methods in Cell Biology (78)

Bibliography

Emanuelsson, O., von Heijne, G., & Schneider, G. (2001). Chapter 10 Analysis and prediction of mitochondrial targeting peptides. Mitochondria, 175â187.

Authors 3

Olof Emanuelsson (first)
Gunnar von Heijne (additional)
Gisbert Schneider (additional)

References 76 Referenced 37

10.1016/S0092-8674(00)80691-1 / Cell / Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20 by Abe (2000)
10.1016/S0014-5793(98)00717-0 / FEBS Lett. / Potential dual targeting of an Arabidopsis archaebacterial-like histidyl-tRNA synthetase to mitochondria and chloroplasts by Akashi (1998)
10.1074/jbc.273.3.1469 / J. Biol. Chem. / Two distinct and independent mitochondrial targeting signals function in the sorting of an inner membrane protein, cytochrome c1 by Arnold (1998)
10.1016/S0021-9258(17)37639-1 / J. Biol. Chem. / Characterization of the mitochondrial processing peptidase of Neurospora crassa by Arretz (1994)
{'key': '10.1016/S0091-679X(01)65011-8_BIB5', 'first-page': '403', 'article-title': 'Processing of mitochondrial precursor proteins', 'volume': '50', 'author': 'Arretz', 'year': '1991', 'journal-title': 'Biomed. Biochim. Acta'} / Biomed. Biochim. Acta / Processing of mitochondrial precursor proteins by Arretz (1991)
10.1093/nar/27.1.49 / Nucleic Acids Res. / The SWISS-PROT protein sequence data bank and its supplement TrEMBL in 1999 by Bairoch (1999)
10.1042/bj3290359 / Biochem. J. / Influence of N-terminal sequence variation on the sorting of major adenylate kinase to the mitochondrial intermembrane space in yeast by Bandlow (1998)
10.1007/BF00282462 / Mol. Gen. Genet. / Mitochondrial inner membrane protease 1 of Saccharomyces cerevisiae shows sequence similarity to the Escherichia coli leader peptidase by Behrens (1991)
10.1074/jbc.274.23.16522 / J. Biol. Chem. / Distribution of binding sequences for the mitochondrial import receptors tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein by Brix (1999)
{'key': '10.1016/S0091-679X(01)65011-8_BIB10', 'series-title': 'Signal Peptidases', 'first-page': '73', 'article-title': 'The mitochondrial processing peptidase', 'author': 'Brunner', 'year': '1994'} / Signal Peptidases / The mitochondrial processing peptidase by Brunner (1994)
10.1093/protein/12.2.107 / Protein Eng. / Protein subcellular location prediction by Chou (1999)
10.1016/S0021-9258(18)47794-0 / J. Biol. Chem. / Mutation of a neutral amino acid in the transit peptide of rat mitochondrial malate dehydrogenase abolishes binding and import by Chu (1987)
{'key': '10.1016/S0091-679X(01)65011-8_BIB13', 'first-page': '441', 'article-title': 'MitoProt, a Macintosh application for studying mitochondrial proteins', 'volume': '11', 'author': 'Claros', 'year': '1995', 'journal-title': 'Comput. Appl. Biosci.'} / Comput. Appl. Biosci. / MitoProt, a Macintosh application for studying mitochondrial proteins by Claros (1995)
10.1016/S0959-440X(97)80057-7 / Curr. Opin. Struct. Biol. / Prediction of N-terminal targeting signals by Claros (1997)
10.1111/j.1432-1033.1996.00779.x / Eur. J. Biochem. / Computational method to predict mitochondrially imported proteins and their targeting sequences by Claros (1996)
10.1074/jbc.273.27.17278 / J. Biol. Chem. / Ribosome binding to mitochondria is regulated by GTP and the transit peptide by Crowley (1998)
10.1074/jbc.274.45.32351 / J. Biol. Chem. / The C terminus of a chloroplast precursor modulates its interaction with the translocation apparatus and PIRAC by Dabney-Smith (1999)
10.1007/BF00019010 / Plant Mol. Biol. / Mitochondrial and chloroplast targeting sequences in tandem modify protein import specificity in plant organelles by De Castro Silva Filho (1996)
{'key': '10.1016/S0091-679X(01)65011-8_BIB19', 'first-page': '11752', 'article-title': 'An internal targeting signal directing proteins into the mitochondrial intermembrane space', 'volume': '96', 'author': 'Diekert', 'year': '1999'} / An internal targeting signal directing proteins into the mitochondrial intermembrane space by Diekert (1999)
10.1006/jmbi.2000.3903 / J. Mol. Biol. / Predicting subcellular localization of proteins based on their N-terminal amino acid sequence by Emanuelsson (2000)
10.1093/emboj/17.22.6508 / EMBO J. / C- to N-terminal translocation of preproteins into mitochondria by Folsch (1998)
10.1002/j.1460-2075.1996.tb00380.x / EMBO J. / Internal targeting signal of the BCS1 protein: A novel mechanism of import into mitochondria by Folsch (1996)
{'key': '10.1016/S0091-679X(01)65011-8_BIB23', 'series-title': 'Genome Informatics', 'first-page': '53', 'article-title': 'Prediction of mitochondrial targeting signals using Hidden Markov Models', 'author': 'Fujiwara', 'year': '1997'} / Genome Informatics / Prediction of mitochondrial targeting signals using Hidden Markov Models by Fujiwara (1997)
{'key': '10.1016/S0091-679X(01)65011-8_BIB24', 'first-page': '267', 'article-title': 'Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps', 'volume': '79', 'author': 'Gasser', 'year': '1982'} / Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps by Gasser (1982)
10.1016/0014-5793(88)81257-2 / FEBS Lett. / Mitonchondrial targeting sequences: Why “non-amphiphilic” peptides may still be amphiphilic by Gavel (1988)
10.1093/protein/4.1.33 / Protein Eng. / Cleavage-site motifs in mitochondrial targeting peptides by Gavel (1990)
10.1023/A:1006020208140 / Plant Mol. Biol. / Mitochondrial protein import in plants: Signals, sorting, targeting, processing and regulation by Glaser (1998)
10.1016/0092-8674(92)90292-K / Cell / Cytochromes c1 and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism by Glick (1992)
10.1021/bi00194a028 / Biochemistry / Structure of the signal sequences for two mitochondrial matrix proteins that are not proteolytically processed upon import by Hammen (1994)
10.1021/bi951848g / Biochemistry / Amphiphilicity determines binding properties of three mitochondrial presequences to lipid surfaces by Hammen (1996)
10.1074/jbc.271.35.21041 / J. Biol. Chem. / The role of positive charges and structural segments in the presequence of rat liver aldehyde dehydrogenase in import into mitochondria by Hammen (1996)
{'key': '10.1016/S0091-679X(01)65011-8_BIB32', 'first-page': '4056', 'article-title': 'Survey of amino-terminal proteolytic cleavage sites in mitochondrial precursor proteins: Leader peptides cleaved by two matrix proteases share a three-amino acid motif', 'volume': '86', 'author': 'Hendrick', 'year': '1989'} / Survey of amino-terminal proteolytic cleavage sites in mitochondrial precursor proteins: Leader peptides cleaved by two matrix proteases share a three-amino acid motif by Hendrick (1989)
10.1002/pro.5560010313 / Protein Sci. / Selection of representative protein data sets by Hobohm (1992)
{'key': '10.1016/S0091-679X(01)65011-8_BIB34', 'first-page': '147', 'article-title': 'Better prediction of protein cellular localization sites with the k nearest neighbors classifier', 'volume': '5', 'author': 'Horton', 'year': '1997', 'journal-title': 'ISMB'} / ISMB / Better prediction of protein cellular localization sites with the k nearest neighbors classifier by Horton (1997)
10.1105/tpc.2.12.1249 / Plant Cell / A yeast mitochondrial leader peptide functions in vivo as a dual targeting signal for both chloroplasts and mitochondria by Huang (1990)
10.1007/BF00020399 / Plant Mol. Biol. / Peculiar properties of the PsaF photosystem I protein from the green alga Chlamydomonas reinhardtii: Presequence independent import of the PsaF protein into both chloroplasts and mitochondria by Hugosson (1995)
{'key': '10.1016/S0091-679X(01)65011-8_BIB37', 'series-title': 'Signal Peptidases', 'first-page': '87', 'article-title': 'Mitochondrial intermediate peptidase', 'author': 'Isaya', 'year': '1994'} / Signal Peptidases / Mitochondrial intermediate peptidase by Isaya (1994)
10.1083/jcb.113.1.65 / J. Cell Biol. / Cleavage of precursors by the mitochondrial processing peptidase requires a compatible mature protein or an intermediate octapeptide by Isaya (1991)
10.1074/jbc.270.3.1323 / J. Biol. Chem. / Solution structure of the acylated and noncleavable mitochondrial targeting signal of rat chaperonin 10 by Jarvis (1995)
{'key': '10.1016/S0091-679X(01)65011-8_BIB40', 'first-page': '7536', 'article-title': 'Two mitochondrial matrix proteases act sequentially in the processing of mammalian matrix enzymes', 'volume': '85', 'author': 'Kalousek', 'year': '1988'} / Two mitochondrial matrix proteases act sequentially in the processing of mammalian matrix enzymes by Kalousek (1988)
10.1016/0014-5793(96)00734-X / FEBS Lett. / NMR structures of a mitochondrial transit peptide from the green alga Chlamydomonas reinhardtii by Lancelin (1996)
10.1074/jbc.274.30.20937 / J. Biol. Chem. / The DNA helicase, Hmi1p, is transported into mitochondria by a C-terminal cleavable targeting signal by Lee (1999)
10.1016/S0021-9258(19)47048-8 / J. Biol. Chem. / The mitochondrial targeting function of randomly generated peptide sequences correlates with predicted helical amphiphilicity by Lemire (1989)
10.1016/0005-2795(75)90109-9 / Biochim. Biophys. Acta / Comparison of the predicted and observed secondary structure of T4 phage lysozyme by Matthews (1975)
10.1042/bj3030657 / Biochem. J. / A spectroscopic study of the mitochondrial transit peptide of rat malate dehydrogenase by McLachlan (1994)
10.1128/MCB.17.11.6574 / Mol. Cell Biol. / The intermembrane space domain of mitochondrial Tom22 functions as a trans binding site for preproteins with N-terminal targeting sequences by Moczko (1997)
10.1016/S0888-7543(05)80111-9 / Genomics / A knowledge base for predicting protein localization sites in eukaryotic cells by Nakai (1992)
10.1146/annurev.biochem.66.1.863 / Annu. Rev. Biochem. / Protein import into mitochondria by Neupert (1997)
10.1126/science.8266095 / Science / A mitochondrial protease with two catalytic subunits of nonover-lapping specificities by Nunnari (1993)
10.1016/S0021-9258(17)31443-6 / J. Biol. Chem. / Structural requirements for recognition of the precursor proteins by the mitochondrial processing peptidase by Ou (1994)
10.1016/0968-0004(94)90113-9 / Trends Biochem. Sci. / The protein import machinery of the mitochondrial inner membrane by Pfanner (1994)
10.1146/annurev.cellbio.13.1.25 / Annu. Rev. Cell Dev. Biol. / Mitochondrial preprotein translocase by Pfanner (1997)
{'key': '10.1016/S0091-679X(01)65011-8_BIB53', 'series-title': 'Signal Peptidases', 'first-page': '105', 'article-title': 'The mitochondrial inner membrane peptidase', 'author': 'Pratje', 'year': '1994'} / Signal Peptidases / The mitochondrial inner membrane peptidase by Pratje (1994)
10.1093/nar/26.9.2230 / Nucleic Acids Res. / Using neural networks for prediction of the subcellular location of proteins by Reinhardt (1998)
10.1023/A:1022403604273 / J. Bioenerg. Biomembr. / Recognition and binding of mitochondrial presequences during the import of proteins into mitochondria by Roise (1997)
10.1074/jbc.273.14.8040 / J. Biol. Chem. / Sorting of D-lactate dehydrogenase to the inner membrane of mitochondria: Analysis of topogenic signal and energetic requirements by Rojo (1998)
10.3109/09687689509072431 / Mol. Membr. Biol. / Protein transport via amino-terminal targeting sequences: Common themes and diverse systems by Rusch (1995)
10.1002/pro.5560020202 / Protein Sci. / The protein import machinery of mitochondria by Schatz (1993)
10.1016/S0014-5793(99)01415-5 / FEBS Lett. / Positively charged residues, the helical conformation and the structural flexibility of the leader sequence of pALDH are important for recognition by hTom20 by Schleiff (1999)
10.1105/tpc.1.8.783 / Plant Cell / A yeast mitochondrial presequence functions as a signal for targeting to plant mitochondria in vivo by Schmitz (1989)
10.1016/S0378-1119(99)00310-8 / Gene / How many potentially secreted proteins are contained in a bacterial genome? by Schneider (1999)
10.1016/S0006-3495(95)80205-5 / Biophys. J. / Peptide design in machina: Development of artificial mitochondrial protein precursor cleavage sites by simulated molecular evolution by Schneider (1995)
10.1002/(SICI)1097-0134(19980101)30:1<49::AID-PROT5>3.0.CO;2-F / Proteins / Feature-extraction from endopeptidase cleavage sites in mitochondrial targeting peptides by Schneider (1998)
10.1016/S0079-6107(98)00026-1 / Prog. Biophys. Mol. Biol. / Artificial neural networks for computer-based molecular design by Schneider (1998)
10.1074/jbc.274.18.12759 / J. Biol. Chem. / The structure of precursor proteins during import into mitochondria by Schwartz (1999)
10.1016/S1360-1385(98)01212-6 / Trends Plant Sci. / Mitochondrial targeting peptides in plants by Sjöling (1998)
10.1074/jbc.270.39.22842 / J. Biol. Chem. / Biogenesis of mitochondrial heme lyases in yeast: Import and folding in the intermembrane space by Steiner (1995)
10.1016/S0021-9258(19)36598-6 / J. Biol. Chem. / Import, processing, and two-dimensional NMR structure of a linker-deleted signal peptide of rat liver mitochondrial aldehyde dehydrogenase by Thornton (1993)
10.1002/j.1460-2075.1987.tb02522.x / EMBO J. / Transport of proteins to the mitochondrial intermembrane space: The ‘matrix-targeting’ and the ‘sorting’ domains in the cytochrome c1 presequence by Van Loon (1987)
10.1016/0968-0004(93)90090-A / Trends Biochem. Sci. / Co-translational import into mitochondria: An alternative view by Verner (1993)
{'key': '10.1016/S0091-679X(01)65011-8_BIB71', 'series-title': 'Concepts in Protein Engineering and Design', 'first-page': '263', 'article-title': 'Design of protein targeting signals and membrane protein engineering', 'author': 'Von Heijne', 'year': '1994'} / Concepts in Protein Engineering and Design / Design of protein targeting signals and membrane protein engineering by Von Heijne (1994)
10.1002/j.1460-2075.1986.tb04364.x / EMBO J. / Mitochondrial targeting sequences may form amphiphilic helices by Von Heijne (1986)
10.1111/j.1432-1033.1989.tb14679.x / Eur. J. Biochem. / Domain structure of mitochondrial and chloroplast targeting peptides by Von Heijne (1989)
10.1016/S0304-4157(99)00007-6 / Biochim. Biophys. Acta / Mechanisms of protein translocation into mitochondria by Voos (1999)
10.1074/jbc.271.35.21226 / J. Biol. Chem. / Influence of the mature portion of a precursor protein on the mitochondrial signal sequence by Waltner (1996)
10.1074/jbc.270.44.26311 / J. Biol. Chem. / Conversion of a nonprocessed mitochondrial precursor protein into one that is processed by the mitochondrial processing peptidase by Waltner (1995)

Dates

Type	When
Created	21 years, 3 months ago (May 19, 2004, 12:16 a.m.)
Deposited	4 years, 2 months ago (June 17, 2021, 4:26 p.m.)
Indexed	1 month ago (July 24, 2025, 8:04 a.m.)
Issued	24 years, 7 months ago (Jan. 1, 2001)
Published	24 years, 7 months ago (Jan. 1, 2001)
Published Print	24 years, 7 months ago (Jan. 1, 2001)

Funders 0

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BibTeX

@inbook{Emanuelsson_2001, title={Chapter 10 Analysis and prediction of mitochondrial targeting peptides}, ISSN={0091-679X}, url={http://dx.doi.org/10.1016/s0091-679x(01)65011-8}, DOI={10.1016/s0091-679x(01)65011-8}, booktitle={Mitochondria}, publisher={Elsevier}, author={Emanuelsson, Olof and von Heijne, Gunnar and Schneider, Gisbert}, year={2001}, pages={175–187} }

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      "doi-asserted-by": "crossref",
      "first-page": "6508",
      "DOI": "10.1093/emboj/17.22.6508",
      "article-title": "C- to N-terminal translocation of preproteins into mitochondria",
      "volume": "17",
      "author": "Folsch",
      "year": "1998",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "479",
      "DOI": "10.1002/j.1460-2075.1996.tb00380.x",
      "article-title": "Internal targeting signal of the BCS1 protein: A novel mechanism of import into mitochondria",
      "volume": "15",
      "author": "Folsch",
      "year": "1996",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB23",
      "series-title": "Genome Informatics",
      "first-page": "53",
      "article-title": "Prediction of mitochondrial targeting signals using Hidden Markov Models",
      "author": "Fujiwara",
      "year": "1997"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB24",
      "first-page": "267",
      "article-title": "Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps",
      "volume": "79",
      "author": "Gasser",
      "year": "1982"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB25",
      "doi-asserted-by": "crossref",
      "first-page": "173",
      "DOI": "10.1016/0014-5793(88)81257-2",
      "article-title": "Mitonchondrial targeting sequences: Why \u201cnon-amphiphilic\u201d peptides may still be amphiphilic",
      "volume": "235",
      "author": "Gavel",
      "year": "1988",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "33",
      "DOI": "10.1093/protein/4.1.33",
      "article-title": "Cleavage-site motifs in mitochondrial targeting peptides",
      "volume": "4",
      "author": "Gavel",
      "year": "1990",
      "journal-title": "Protein Eng."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "311",
      "DOI": "10.1023/A:1006020208140",
      "article-title": "Mitochondrial protein import in plants: Signals, sorting, targeting, processing and regulation",
      "volume": "38",
      "author": "Glaser",
      "year": "1998",
      "journal-title": "Plant Mol. Biol."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB28",
      "doi-asserted-by": "crossref",
      "first-page": "809",
      "DOI": "10.1016/0092-8674(92)90292-K",
      "article-title": "Cytochromes c1 and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism",
      "volume": "69",
      "author": "Glick",
      "year": "1992",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "8610",
      "DOI": "10.1021/bi00194a028",
      "article-title": "Structure of the signal sequences for two mitochondrial matrix proteins that are not proteolytically processed upon import",
      "volume": "33",
      "author": "Hammen",
      "year": "1994",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "3772",
      "DOI": "10.1021/bi951848g",
      "article-title": "Amphiphilicity determines binding properties of three mitochondrial presequences to lipid surfaces",
      "volume": "35",
      "author": "Hammen",
      "year": "1996",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "21041",
      "DOI": "10.1074/jbc.271.35.21041",
      "article-title": "The role of positive charges and structural segments in the presequence of rat liver aldehyde dehydrogenase in import into mitochondria",
      "volume": "271",
      "author": "Hammen",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB32",
      "first-page": "4056",
      "article-title": "Survey of amino-terminal proteolytic cleavage sites in mitochondrial precursor proteins: Leader peptides cleaved by two matrix proteases share a three-amino acid motif",
      "volume": "86",
      "author": "Hendrick",
      "year": "1989"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "409",
      "DOI": "10.1002/pro.5560010313",
      "article-title": "Selection of representative protein data sets",
      "volume": "1",
      "author": "Hobohm",
      "year": "1992",
      "journal-title": "Protein Sci."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB34",
      "first-page": "147",
      "article-title": "Better prediction of protein cellular localization sites with the k nearest neighbors classifier",
      "volume": "5",
      "author": "Horton",
      "year": "1997",
      "journal-title": "ISMB"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB35",
      "doi-asserted-by": "crossref",
      "first-page": "1249",
      "DOI": "10.1105/tpc.2.12.1249",
      "article-title": "A yeast mitochondrial leader peptide functions in vivo as a dual targeting signal for both chloroplasts and mitochondria",
      "volume": "2",
      "author": "Huang",
      "year": "1990",
      "journal-title": "Plant Cell"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB36",
      "doi-asserted-by": "crossref",
      "first-page": "525",
      "DOI": "10.1007/BF00020399",
      "article-title": "Peculiar properties of the PsaF photosystem I protein from the green alga Chlamydomonas reinhardtii: Presequence independent import of the PsaF protein into both chloroplasts and mitochondria",
      "volume": "28",
      "author": "Hugosson",
      "year": "1995",
      "journal-title": "Plant Mol. Biol."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB37",
      "series-title": "Signal Peptidases",
      "first-page": "87",
      "article-title": "Mitochondrial intermediate peptidase",
      "author": "Isaya",
      "year": "1994"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB38",
      "doi-asserted-by": "crossref",
      "first-page": "65",
      "DOI": "10.1083/jcb.113.1.65",
      "article-title": "Cleavage of precursors by the mitochondrial processing peptidase requires a compatible mature protein or an intermediate octapeptide",
      "volume": "113",
      "author": "Isaya",
      "year": "1991",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB39",
      "doi-asserted-by": "crossref",
      "first-page": "1323",
      "DOI": "10.1074/jbc.270.3.1323",
      "article-title": "Solution structure of the acylated and noncleavable mitochondrial targeting signal of rat chaperonin 10",
      "volume": "270",
      "author": "Jarvis",
      "year": "1995",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB40",
      "first-page": "7536",
      "article-title": "Two mitochondrial matrix proteases act sequentially in the processing of mammalian matrix enzymes",
      "volume": "85",
      "author": "Kalousek",
      "year": "1988"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB41",
      "doi-asserted-by": "crossref",
      "first-page": "203",
      "DOI": "10.1016/0014-5793(96)00734-X",
      "article-title": "NMR structures of a mitochondrial transit peptide from the green alga Chlamydomonas reinhardtii",
      "volume": "391",
      "author": "Lancelin",
      "year": "1996",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB42",
      "doi-asserted-by": "crossref",
      "first-page": "20937",
      "DOI": "10.1074/jbc.274.30.20937",
      "article-title": "The DNA helicase, Hmi1p, is transported into mitochondria by a C-terminal cleavable targeting signal",
      "volume": "274",
      "author": "Lee",
      "year": "1999",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB43",
      "doi-asserted-by": "crossref",
      "first-page": "20206",
      "DOI": "10.1016/S0021-9258(19)47048-8",
      "article-title": "The mitochondrial targeting function of randomly generated peptide sequences correlates with predicted helical amphiphilicity",
      "volume": "264",
      "author": "Lemire",
      "year": "1989",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB44",
      "doi-asserted-by": "crossref",
      "first-page": "442",
      "DOI": "10.1016/0005-2795(75)90109-9",
      "article-title": "Comparison of the predicted and observed secondary structure of T4 phage lysozyme",
      "volume": "405",
      "author": "Matthews",
      "year": "1975",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB45",
      "doi-asserted-by": "crossref",
      "first-page": "657",
      "DOI": "10.1042/bj3030657",
      "article-title": "A spectroscopic study of the mitochondrial transit peptide of rat malate dehydrogenase",
      "volume": "303",
      "author": "McLachlan",
      "year": "1994",
      "journal-title": "Biochem. J."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB46",
      "doi-asserted-by": "crossref",
      "first-page": "6574",
      "DOI": "10.1128/MCB.17.11.6574",
      "article-title": "The intermembrane space domain of mitochondrial Tom22 functions as a trans binding site for preproteins with N-terminal targeting sequences",
      "volume": "17",
      "author": "Moczko",
      "year": "1997",
      "journal-title": "Mol. Cell Biol."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB47",
      "doi-asserted-by": "crossref",
      "first-page": "897",
      "DOI": "10.1016/S0888-7543(05)80111-9",
      "article-title": "A knowledge base for predicting protein localization sites in eukaryotic cells",
      "volume": "14",
      "author": "Nakai",
      "year": "1992",
      "journal-title": "Genomics"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB48",
      "doi-asserted-by": "crossref",
      "first-page": "863",
      "DOI": "10.1146/annurev.biochem.66.1.863",
      "article-title": "Protein import into mitochondria",
      "volume": "66",
      "author": "Neupert",
      "year": "1997",
      "journal-title": "Annu. Rev. Biochem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB49",
      "doi-asserted-by": "crossref",
      "first-page": "1997",
      "DOI": "10.1126/science.8266095",
      "article-title": "A mitochondrial protease with two catalytic subunits of nonover-lapping specificities",
      "volume": "262",
      "author": "Nunnari",
      "year": "1993",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB50",
      "doi-asserted-by": "crossref",
      "first-page": "24673",
      "DOI": "10.1016/S0021-9258(17)31443-6",
      "article-title": "Structural requirements for recognition of the precursor proteins by the mitochondrial processing peptidase",
      "volume": "269",
      "author": "Ou",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB51",
      "doi-asserted-by": "crossref",
      "first-page": "368",
      "DOI": "10.1016/0968-0004(94)90113-9",
      "article-title": "The protein import machinery of the mitochondrial inner membrane",
      "volume": "19",
      "author": "Pfanner",
      "year": "1994",
      "journal-title": "Trends Biochem. Sci."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB52",
      "doi-asserted-by": "crossref",
      "first-page": "25",
      "DOI": "10.1146/annurev.cellbio.13.1.25",
      "article-title": "Mitochondrial preprotein translocase",
      "volume": "13",
      "author": "Pfanner",
      "year": "1997",
      "journal-title": "Annu. Rev. Cell Dev. Biol."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB53",
      "series-title": "Signal Peptidases",
      "first-page": "105",
      "article-title": "The mitochondrial inner membrane peptidase",
      "author": "Pratje",
      "year": "1994"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB54",
      "doi-asserted-by": "crossref",
      "first-page": "2230",
      "DOI": "10.1093/nar/26.9.2230",
      "article-title": "Using neural networks for prediction of the subcellular location of proteins",
      "volume": "26",
      "author": "Reinhardt",
      "year": "1998",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB55",
      "doi-asserted-by": "crossref",
      "first-page": "19",
      "DOI": "10.1023/A:1022403604273",
      "article-title": "Recognition and binding of mitochondrial presequences during the import of proteins into mitochondria",
      "volume": "29",
      "author": "Roise",
      "year": "1997",
      "journal-title": "J. Bioenerg. Biomembr."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB56",
      "doi-asserted-by": "crossref",
      "first-page": "8040",
      "DOI": "10.1074/jbc.273.14.8040",
      "article-title": "Sorting of D-lactate dehydrogenase to the inner membrane of mitochondria: Analysis of topogenic signal and energetic requirements",
      "volume": "273",
      "author": "Rojo",
      "year": "1998",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB57",
      "doi-asserted-by": "crossref",
      "first-page": "295",
      "DOI": "10.3109/09687689509072431",
      "article-title": "Protein transport via amino-terminal targeting sequences: Common themes and diverse systems",
      "volume": "12",
      "author": "Rusch",
      "year": "1995",
      "journal-title": "Mol. Membr. Biol."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB58",
      "doi-asserted-by": "crossref",
      "first-page": "141",
      "DOI": "10.1002/pro.5560020202",
      "article-title": "The protein import machinery of mitochondria",
      "volume": "2",
      "author": "Schatz",
      "year": "1993",
      "journal-title": "Protein Sci."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB59",
      "doi-asserted-by": "crossref",
      "first-page": "9",
      "DOI": "10.1016/S0014-5793(99)01415-5",
      "article-title": "Positively charged residues, the helical conformation and the structural flexibility of the leader sequence of pALDH are important for recognition by hTom20",
      "volume": "461",
      "author": "Schleiff",
      "year": "1999",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB60",
      "doi-asserted-by": "crossref",
      "first-page": "783",
      "DOI": "10.1105/tpc.1.8.783",
      "article-title": "A yeast mitochondrial presequence functions as a signal for targeting to plant mitochondria in vivo",
      "volume": "1",
      "author": "Schmitz",
      "year": "1989",
      "journal-title": "Plant Cell"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB61",
      "doi-asserted-by": "crossref",
      "first-page": "113",
      "DOI": "10.1016/S0378-1119(99)00310-8",
      "article-title": "How many potentially secreted proteins are contained in a bacterial genome?",
      "volume": "237",
      "author": "Schneider",
      "year": "1999",
      "journal-title": "Gene"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB62",
      "doi-asserted-by": "crossref",
      "first-page": "434",
      "DOI": "10.1016/S0006-3495(95)80205-5",
      "article-title": "Peptide design in machina: Development of artificial mitochondrial protein precursor cleavage sites by simulated molecular evolution",
      "volume": "68",
      "author": "Schneider",
      "year": "1995",
      "journal-title": "Biophys. J."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB63",
      "doi-asserted-by": "crossref",
      "first-page": "49",
      "DOI": "10.1002/(SICI)1097-0134(19980101)30:1<49::AID-PROT5>3.0.CO;2-F",
      "article-title": "Feature-extraction from endopeptidase cleavage sites in mitochondrial targeting peptides",
      "volume": "30",
      "author": "Schneider",
      "year": "1998",
      "journal-title": "Proteins"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB64",
      "doi-asserted-by": "crossref",
      "first-page": "175",
      "DOI": "10.1016/S0079-6107(98)00026-1",
      "article-title": "Artificial neural networks for computer-based molecular design",
      "volume": "70",
      "author": "Schneider",
      "year": "1998",
      "journal-title": "Prog. Biophys. Mol. Biol."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB65",
      "doi-asserted-by": "crossref",
      "first-page": "12759",
      "DOI": "10.1074/jbc.274.18.12759",
      "article-title": "The structure of precursor proteins during import into mitochondria",
      "volume": "274",
      "author": "Schwartz",
      "year": "1999",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB66",
      "doi-asserted-by": "crossref",
      "first-page": "136",
      "DOI": "10.1016/S1360-1385(98)01212-6",
      "article-title": "Mitochondrial targeting peptides in plants",
      "volume": "3",
      "author": "Sj\u00f6ling",
      "year": "1998",
      "journal-title": "Trends Plant Sci."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB67",
      "doi-asserted-by": "crossref",
      "first-page": "22842",
      "DOI": "10.1074/jbc.270.39.22842",
      "article-title": "Biogenesis of mitochondrial heme lyases in yeast: Import and folding in the intermembrane space",
      "volume": "270",
      "author": "Steiner",
      "year": "1995",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB68",
      "doi-asserted-by": "crossref",
      "first-page": "19906",
      "DOI": "10.1016/S0021-9258(19)36598-6",
      "article-title": "Import, processing, and two-dimensional NMR structure of a linker-deleted signal peptide of rat liver mitochondrial aldehyde dehydrogenase",
      "volume": "268",
      "author": "Thornton",
      "year": "1993",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB69",
      "doi-asserted-by": "crossref",
      "first-page": "2433",
      "DOI": "10.1002/j.1460-2075.1987.tb02522.x",
      "article-title": "Transport of proteins to the mitochondrial intermembrane space: The \u2018matrix-targeting\u2019 and the \u2018sorting\u2019 domains in the cytochrome c1 presequence",
      "volume": "6",
      "author": "Van Loon",
      "year": "1987",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB70",
      "doi-asserted-by": "crossref",
      "first-page": "366",
      "DOI": "10.1016/0968-0004(93)90090-A",
      "article-title": "Co-translational import into mitochondria: An alternative view",
      "volume": "18",
      "author": "Verner",
      "year": "1993",
      "journal-title": "Trends Biochem. Sci."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB71",
      "series-title": "Concepts in Protein Engineering and Design",
      "first-page": "263",
      "article-title": "Design of protein targeting signals and membrane protein engineering",
      "author": "Von Heijne",
      "year": "1994"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB72",
      "doi-asserted-by": "crossref",
      "first-page": "1335",
      "DOI": "10.1002/j.1460-2075.1986.tb04364.x",
      "article-title": "Mitochondrial targeting sequences may form amphiphilic helices",
      "volume": "5",
      "author": "Von Heijne",
      "year": "1986",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB73",
      "doi-asserted-by": "crossref",
      "first-page": "535",
      "DOI": "10.1111/j.1432-1033.1989.tb14679.x",
      "article-title": "Domain structure of mitochondrial and chloroplast targeting peptides",
      "volume": "180",
      "author": "Von Heijne",
      "year": "1989",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB74",
      "doi-asserted-by": "crossref",
      "first-page": "235",
      "DOI": "10.1016/S0304-4157(99)00007-6",
      "article-title": "Mechanisms of protein translocation into mitochondria",
      "volume": "1422",
      "author": "Voos",
      "year": "1999",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0091-679X(01)65011-8_BIB75",
      "doi-asserted-by": "crossref",
      "first-page": "21226",
      "DOI": "10.1074/jbc.271.35.21226",
      "article-title": "Influence of the mature portion of a precursor protein on the mitochondrial signal sequence",
      "volume": "271",
      "author": "Waltner",
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