DOI: 10.1016/s0022-2836(03)00782-4. Inverting Enantioselectivity of Burkholderia cepacia KWI-56 Lipase by Combinatorial Mutation and High-throughput Screening Using Single-molecule PCR and In Vitro Expression

Inverting Enantioselectivity of Burkholderia cepacia KWI-56 Lipase by Combinatorial Mutation and High-throughput Screening Using Single-molecule PCR and In Vitro Expression

10.1016/s0022-2836(03)00782-4

Crossref journal-article

Elsevier BV

Journal of Molecular Biology (78)

Bibliography

Koga, Y., Kato, K., Nakano, H., & Yamane, T. (2003). Inverting Enantioselectivity of Burkholderia cepacia KWI-56 Lipase by Combinatorial Mutation and High-throughput Screening Using Single-molecule PCR and In Vitro Expression. Journal of Molecular Biology, 331(3), 585â592.

Authors 4

Yuichi Koga (first)
Katsuya Kato (additional)
Hideo Nakano (additional)
Tsuneo Yamane (additional)

References 23 Referenced 90

10.1110/ps.13501 / Protein Sci. / Rational design of enantioselective enzymes requires considerations of entropy by Ottosson (2001)
10.1021/jo981783y / J. Org. Chem. / Molecular basis for enantioselctivity of lipase from Pseudomonas cepacia toward primary alcohols. Modeling, kinetics, and chemical modification of Tyr29 to increase or decrease enantioselectivity by Tumoi (1999)
10.1016/S0167-4838(00)00239-9 / Biochim. Biophys. Acta / Lipase protein engineering by Svendsen (2000)
10.1021/ja015604x / J. Am. Chem. Soc. / Creation of an enantioselective hydrolase by engineered substrate-assisted catalysis by Magnusson (2001)
10.1002/1439-7633(20011001)2:10<766::AID-CBIC766>3.0.CO;2-K / ChemBioChem / Improved enantioselectivity of a lipase by rational protein engineering by Rotticci (2001)
10.1038/73773 / Nature Biotechnol. / Inverting enantioselectivity by directed evolution of hydantoinase for improved production of l-methionine by May (2000)
10.1002/1521-3773(20011001)40:19<3589::AID-ANIE3589>3.0.CO;2-X / Angew. Chem. Int. Ed. / Directed evolution of an enantioselective enzyme through combinatorial multiple-cassette mutagenesis by Reetz (2001)
10.1039/b109913m / Chem. Commun. / Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution by Zha (2001)
10.1271/bbb1961.55.2349 / Agric. Biol. Chem. / Cloning, nucleotide sequencing, and expression in Escherichia coli of a lipase and its activator genes from Pseudomonas sp. KWI-56 by Iizumi (1991)
10.1271/bbb.58.1023 / Biosci. Biotechnol. Biochem. / Role of the gene encoding lipase activator from Pseudomonas sp. strain KWI-56 in in vitro activation of lipase by Iizumi (1994)
10.1016/S0958-1669(00)00199-3 / Curr. Opin. Biotechnol. / Advances in Escherichia coli production of therapeutic proteins by Swartz (2001)
10.1128/JB.182.2.295-302.2000 / J. Bacteriol. / In vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase by Yang (2000)
10.1093/protein/15.2.147 / Protein Eng. / Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding site by Yang (2002)
{'key': '10.1016/S0022-2836(03)00782-4_BIB14', 'first-page': '71', 'volume': '81', 'author': 'Nakano', 'year': '2003', 'journal-title': 'Seibutsu-kougaku Kaishi'} / Seibutsu-kougaku Kaishi by Nakano (2003)
10.1016/S0022-2836(02)00094-3 / J. Mol. Biol. / High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression by Rungpragayphan (2002)
10.1016/S1389-1723(02)80122-0 / J. Biosci. Bioeng. / In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecular PCR and cell-free protein synthesis by Koga (2002)
10.1016/S0969-2126(97)00177-9 / Structure / The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor by Kim (1997)
10.1016/S0014-5793(03)00251-5 / FEBS Letters / PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries by Rungpragayphan (2003)
10.1046/j.1432-1327.1998.2540333.x / Eur. J. Biochem. / Structural basis of the chiral selectivity of Pseudomonas cepacia lipase by Lang (1998)
10.1016/S0006-3495(98)77839-7 / Biophys. J. / Molecular modeling of the enantioselectivity in lipase-catalyzed transesterification reactions by Hœffner (1998)
{'key': '10.1016/S0022-2836(03)00782-4_BIB21', 'article-title': 'Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase', 'author': 'Imamura-Miyazaki', 'year': '2003', 'journal-title': 'Protein Eng.'} / Protein Eng. / Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase by Imamura-Miyazaki (2003)
10.1128/JB.181.9.2914-2921.1999 / J. Bacteriol. / Analysis of a polyhydroxyalkanoic acid granule-associated 16-kilodalton protein and its putative regulator in the pha locus of Paracoccus denitroficans by Maehara (1999)
{'key': '10.1016/S0022-2836(03)00782-4_BIB23', 'first-page': '800', 'article-title': 'pBBR1MCS: a broad-host range cloning vector', 'volume': '16', 'author': 'Kovach', 'year': '1994', 'journal-title': 'Bio/Techniques'} / Bio/Techniques / pBBR1MCS: a broad-host range cloning vector by Kovach (1994)

Dates

Type	When
Created	22 years ago (Aug. 7, 2003, 10:35 p.m.)
Deposited	5 years, 5 months ago (March 25, 2020, 1:29 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 16, 2024, 4:21 p.m.)
Issued	22 years, 1 month ago (Aug. 1, 2003)
Published	22 years, 1 month ago (Aug. 1, 2003)
Published Print	22 years, 1 month ago (Aug. 1, 2003)

Funders 0

None

BibTeX

@article{Koga_2003, title={Inverting Enantioselectivity of Burkholderia cepacia KWI-56 Lipase by Combinatorial Mutation and High-throughput Screening Using Single-molecule PCR and In Vitro Expression}, volume={331}, ISSN={0022-2836}, url={http://dx.doi.org/10.1016/s0022-2836(03)00782-4}, DOI={10.1016/s0022-2836(03)00782-4}, number={3}, journal={Journal of Molecular Biology}, publisher={Elsevier BV}, author={Koga, Yuichi and Kato, Katsuya and Nakano, Hideo and Yamane, Tsuneo}, year={2003}, month=aug, pages={585–592} }

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