DOI: 10.1016/s0022-2836(03)00232-8. Computational Approach for Prediction of Domain Organization and Substrate Specificity of Modular Polyketide Synthases

Computational Approach for Prediction of Domain Organization and Substrate Specificity of Modular Polyketide Synthases

10.1016/s0022-2836(03)00232-8

Crossref journal-article

Elsevier BV

Journal of Molecular Biology (78)

Bibliography

Yadav, G., Gokhale, R. S., & Mohanty, D. (2003). Computational Approach for Prediction of Domain Organization and Substrate Specificity of Modular Polyketide Synthases. Journal of Molecular Biology, 328(2), 335â363.

Authors 3

Gitanjali Yadav (first)
Rajesh S Gokhale (additional)
Debasisa Mohanty (additional)

References 76 Referenced 188

10.1016/1074-5521(95)90214-7 / Chem. Biol. / Combinatorial biosynthesis of unnatural natural products: the polyketide example by Tsoi (1995)
10.1038/314642a0 / Nature / Production of hybrid antibiotics by genetic engineering by Hopwood (1985)
10.1146/annurev.mi.47.100193.004303 / Annu. Rev. Microbiol. / Polyketide synthesis: prospects for hybrid antibiotics by Katz (1993)
10.1146/annurev.ge.24.120190.000345 / Annu. Rev. Genet. / Molecular genetics of polyketides and its comparison with Fatty acid biosynthesis by Hopwood (1990)
10.1021/cr960034i / Chem. Rev. / Genetic contributions to understanding polyketide synthases by Hopwood (1997)
10.1126/science.284.5413.482 / Science / Dissecting and exploiting intermodular communication in polyketide synthases by Gokhale (1999)
10.1016/S1367-5931(99)00046-0 / Curr. Opin. Chem. Biol. / Role of linkers in communication between protein modules by Gokhale (2000)
10.1016/S1074-5521(00)00041-7 / Chem. Biol. / Structural control of polyketide formation in plant-specific polyketide synthases by Jez (2000)
10.1146/annurev.mi.49.100195.001221 / Annu. Rev. Microbiol. / Polyketide synthase gene manipulation: a structure–function approach in engineering novel antibiotics by Hutchinson (1995)
10.1007/BF01721327 / Mol. Divers. / Antibiotic activity of polyketide products derived from combinatorial biosynthesis: implications for directed evolution by Fu (1996)
10.1146/annurev.biochem.68.1.219 / Annu. Rev. Biochem. / Tolerance and specificity of polyketide synthases by Khosla (1999)
10.1038/375549a0 / Nature / Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits by McDaniel (1995)
10.1016/0167-7799(96)10046-9 / Trends Biotechnol. / Generation of polyketide libraries via combinatorial biosynthesis by Khosla (1996)
10.1016/S1074-5521(00)80020-4 / Chem. Biol. / Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: a route to simple statin analogues by Ranganathan (1999)
10.1016/S1074-5521(97)90222-2 / Chem. Biol. / Engineered intermodular and intramodular polyketide synthase fusions by McDaniel (1997)
10.1126/science.2024119 / Science / Modular organization of the genes required for complex polyketide biosynthesis by Donadio (1991)
10.1073/pnas.90.15.7119 / Proc. Natl Acad. Sci. USA / An erythromycin analog produced by reprogramming of the polyketide synthesis by Donadio (1993)
10.7164/antibiotics.44.357 / J. Antibiot. (Tokyo) / Novel avermectins produced by mutational biosynthesis by Dutton (1991)
10.1016/S1074-5521(96)90069-1 / Chem. Biol. / A hybrid modular polyketide synthase obtained by domain swapping by Oliynyk (1996)
10.1073/pnas.96.5.1846 / Proc. Natl Acad. Sci. USA / Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel unnatural natural products by McDaniel (1999)
10.1128/jb.179.20.6416-6425.1997 / J. Bacteriol. / Acyltransferase domain substitutions in erythromycin polyketide synthase yield novel erythromycin derivatives by Ruan (1997)
10.1021/ja972451y / J. Am. Chem. Soc. / Biosynthesis of 2-nor-6-deoxyerythronolide B by rationally designed domain substitution by Liu (1997)
10.1073/pnas.95.13.7305 / Proc. Natl Acad. Sci. USA / Ethyl substituted erythromycin derivatives produced by directed metabolic engineering by Stassi (1998)
10.1126/science.279.5348.199 / Science / Engineering broader specificity into an antibiotic producing polyketide synthase by Marsden (1998)
10.1126/science.277.5324.367 / Science / Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase by Jacobsen (1997)
10.1021/bi9820311 / Biochemistry / Dissecting the role of acyltransferase domains of modular polyketide synthases in the choice and stereochemical fate of extender units by Lau (1999)
10.1021/bi015864r / Biochemistry / Alteration of the substrate specificity of a modular polyketide synthase acyltransferase domain through site-specific mutations by Reeves (2001)
10.1039/a909079g / Nature Prod. Rep. / Polyketide biosynthesis: a millennium review by Staunton (2001)
10.1126/science.282.5386.63 / Science / Harnessing the biosynthetic code: combinations, permutations and mutations by Cane (1998)
10.1006/jmbi.2000.4272 / J. Mol. Biol. / The crystal structure of β-keto-acyl-carrier protein synthase II from Synechocystis sp at 1.54Å resolution and its relationship to other condensing enzymes by Moche (2001)
10.1073/pnas.011399198 / Proc. Natl Acad. Sci. USA / Crystal structure of the macrocycle forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel by Tsai (2002)
10.1074/jbc.270.22.12961 / J. Biol. Chem. / The E.coli malonyl-CoA: acyl carrier protein transacylase at 1.5Å resolution. Crystal structure of a FAS component by Serre (1995)
10.1021/bi970006+ / Biochemistry / Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2) by Crump (1997)
10.1093/nar/30.1.281 / Nucl. Acids Res. / CDD: a database of conserved domain alignments with links to domain three-dimensional structure by Marchler-Bauer (2002)
10.1073/pnas.92.17.7839 / Proc. Natl Acad. Sci. USA / The biosynthetic gene cluster for the polyketide immunosuppresant rapamycin by Schwecke (1995)
10.1016/0014-5793(95)01119-Y / FEBS Letters / Divergent sequence motifs correlated with the substrate specificity of (methyl) malonyl CoA:acyl carrier protein transacylase domains in modular polyketide synthases by Haydock (1995)
10.1073/pnas.96.17.9509 / Proc. Natl Acad. Sci. USA / Organization of the biosynthetic gene cluster for the polyketide antihelmintic macrolide avermectin in Streptomyces avermitilis by Ikeda (1999)
10.1111/j.1432-1033.1992.tb16603.x / Eur. J. Biochem. / 6-Deoxyerythronolide-B synthase 2 from Saccharpolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme by Bevitt (1992)
10.1128/jb.179.23.7515-7522.1997 / J. Bacteriol. / Identification and characterization of the niddamycin polyketide synthase genes from Streptomyces caelestis by Kakavas (1997)
10.1016/S1074-5521(00)00056-9 / Chem. Biol. / Novel features in a combined polyketide synthase/non-ribosomal peptide synthetase: the myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sga15 by Silakowski (2001)
10.1016/S0378-1119(98)00338-2 / Gene / Characterization of the enzymatic domains in the modular polyketide synthase involved in rifamycin B biosynthesis by Amycolatopsis mediterrannei by Li (1998)
10.1016/S1074-5521(00)00120-4 / Chem. Biol. / Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway by Brautaset (2000)
10.1016/0378-1119(92)90602-L / Gene / Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea by Donadio (1992)
10.1016/S0378-1119(97)00501-5 / Gene / Identification and sequence analysis of the genes encoding a polyketide synthase required for pyoluteorin biosynthesis in Pseudomonas fluorescens Pf-5 by Thompson (1997)
10.1074/jbc.274.52.37391 / J. Biol. Chem. / New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1 by Silakowski (1999)
10.1006/jmbi.1999.2583 / J. Mol. Biol. / GenTHREADER: an efficient and reliable protein fold recognition method for genomic sequences by Jones (1999)
10.1046/j.1365-2958.2002.02815.x / Mol. Microbiol. / Engineering specificity of starter unit selection by the erythromycin-producing polyketide synthase by Long (2002)
10.1016/S1074-5521(00)00091-0 / Chem. Biol. / Predictive, structure-based model of amino acid recognition by nonribosomal peptide synthetase adenylation domains by Challis (2000)
10.1074/jbc.272.18.11975 / J. Biol. Chem. / Alteration of substrate specificity of the malonyl CoA/acetyl CoA: acyl carrier protein s-acyltransferase domain of the multifunctional fatty acid synthase by a mutation of a single arginine residue by Rangan (1997)
10.1016/S1074-5521(01)00087-4 / Chem. Biol. / Chain initiation on the soraphen producing modular polyketide synthase from Sorangium cellulosum by Wilkinson (2001)
10.1128/jb.177.13.3673-3679.1995 / J. Bacteriol. / A Sorangium cellulosum (myxobacterium) gene cluster for biosynthesis of macrolide antibiotic soraphen A: cloning, characterization, and homology to polyketide synthase genes from actinomycetes by Schupp (1995)
10.1074/jbc.M111738200 / J. Biol. Chem. / The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase by Gaitatzis (2002)
10.1016/0378-1119(93)90347-6 / Gene / A Bacillus subtilis large ORF coding for a polypeptide highly similar to polyketide synthases by Scotti (1993)
10.1074/jbc.272.27.16741 / J. Biol. Chem. / Gene knock-out reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria by Azad (1997)
10.1021/bi012086u / Biochemistry / Quantitative analysis of the relative contributions of donor acyl carrier proteins, acceptor ketosynthases, and linker regions to intermodular transfer of intermediates in hybrid polyketide synthases by Wu (2002)
10.1016/S1074-5521(01)00046-1 / Chem. Biol. / Amphotericin biosynthesis in Sterptomyces nodosus: deductions from analysis of polyketide synthase and late genes by Caffrey (2001)
10.1016/S0378-1119(00)00171-2 / Gene / The Fk520 gene cluster of Streptomycetes hygroscopiocus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units by Wu (2000)
10.1016/S0021-9258(17)37226-5 / J. Biol. Chem. / Limited Proteolysis and active-site studies of the first multienzyme component of the erythromycin-producing polyketide synthase by Aparicio (1994)
10.1038/348176a0 / Nature / An unusually large multifuctional polypeptide in the erythromycin-producing PKS of Saccharopolyspora erythraea by Cortes (1997)
10.1016/S1074-5521(00)00075-2 / Chem. Biol. / The biosynthetic gene cluster for the microtubule-stabilizing agents epothilones A and B from Sorangium cellulosum So ce90 by Molnar (2000)
10.1046/j.1365-2958.2000.02059.x / Mol. Microbiol. / Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea by Volchegursky (2000)
10.7164/antibiotics.53.502 / J. Antibiot. (Tokyo) / Cloning, characterization and heterologous expression of a polyketide synthase and P-450 oxidase involved in the biosynthesis of the antibiotic oleandomycin by Shah (2000)
10.1016/S0378-1119(00)00003-2 / Gene / Genetic architecture of the polyketide synthases for methymycin and pikromycin series macrolides by Xue (2000)
10.1016/S1074-5521(00)00038-7 / Chem. Biol. / A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis by Aparicio (2000)
10.1016/S1074-5521(98)90141-7 / Chem. Biol. / Biosynthesis of the ansamycin antibiotic rifamycin, deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699 by August (1998)
10.1016/S1074-5521(01)00029-1 / Chem. Biol. / Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa by Waldron (2001)
10.1023/A:1012065300116 / Antonie Van Leeuwenhoek / The tylosin-biosynthetic genes of Streptomyces fradiae by Cundliffe (2001)
10.1093/nar/30.1.13 / Nucl. Acids Res. / Database resources of the National Center for Biotechnology Information: 2002 update by Wheeler (2002)
10.1016/S0022-2836(05)80360-2 / J. Mol. Biol. / Basic local alignment search tool by Altschul (1990)
10.1093/nar/30.1.276 / Nucl. Acids Res. / The Pfam Protein Families Database by Bateman (2002)
10.1093/nar/22.22.4673 / Nucl. Acids Res. / CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice by Higgins (1994)
10.1093/nar/28.1.235 / Nucl. Acids Res. / The Protein Data Bank by Berman (2000)
10.1006/jmbi.1996.0167 / J. Mol. Biol. / An evolutionary trace method defines binding surfaces common to protein families by Litcharge (1996)
10.1006/jmbi.1997.0926 / J. Mol. Biol. / Side-chain prediction from a backbone-dependent rotamer library: a new tool for homology modeling by Bower (1997)
{'year': '1994', 'series-title': 'Insight II User Guide, Version 2.3.5', 'key': '10.1016/S0022-2836(03)00232-8_BIB75'} / Insight II User Guide, Version 2.3.5 (1994)
10.1002/prot.340040106 / Proteins: Struct. Funct. Genet. / Structure and energetics of ligand binding to proteins: E.coli dihydrofolate reductase–trimethoprim, a drug–receptor system by Dauber-Osguthorpe (1988)

Dates

Type	When
Created	22 years, 4 months ago (April 23, 2003, 5:27 p.m.)
Deposited	4 years, 2 months ago (June 6, 2021, 3:11 p.m.)
Indexed	3 months, 1 week ago (May 22, 2025, 4:46 a.m.)
Issued	22 years, 5 months ago (April 1, 2003)
Published	22 years, 5 months ago (April 1, 2003)
Published Print	22 years, 5 months ago (April 1, 2003)

Funders 0

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BibTeX

@article{Yadav_2003, title={Computational Approach for Prediction of Domain Organization and Substrate Specificity of Modular Polyketide Synthases}, volume={328}, ISSN={0022-2836}, url={http://dx.doi.org/10.1016/s0022-2836(03)00232-8}, DOI={10.1016/s0022-2836(03)00232-8}, number={2}, journal={Journal of Molecular Biology}, publisher={Elsevier BV}, author={Yadav, Gitanjali and Gokhale, Rajesh S and Mohanty, Debasisa}, year={2003}, month=apr, pages={335–363} }

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      "article-title": "Polyketide synthesis: prospects for hybrid antibiotics",
      "volume": "47",
      "author": "Katz",
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      "article-title": "Molecular genetics of polyketides and its comparison with Fatty acid biosynthesis",
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      "article-title": "Genetic contributions to understanding polyketide synthases",
      "volume": "97",
      "author": "Hopwood",
      "year": "1997",
      "journal-title": "Chem. Rev."
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      "first-page": "482",
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      "article-title": "Dissecting and exploiting intermodular communication in polyketide synthases",
      "volume": "284",
      "author": "Gokhale",
      "year": "1999",
      "journal-title": "Science"
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      "volume": "4",
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      "volume": "7",
      "author": "Jez",
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      "author": "Fu",
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      "author": "Dutton",
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      "author": "McDaniel",
      "year": "1999",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "6416",
      "DOI": "10.1128/jb.179.20.6416-6425.1997",
      "article-title": "Acyltransferase domain substitutions in erythromycin polyketide synthase yield novel erythromycin derivatives",
      "volume": "179",
      "author": "Ruan",
      "year": "1997",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "10553",
      "DOI": "10.1021/ja972451y",
      "article-title": "Biosynthesis of 2-nor-6-deoxyerythronolide B by rationally designed domain substitution",
      "volume": "119",
      "author": "Liu",
      "year": "1997",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "7305",
      "DOI": "10.1073/pnas.95.13.7305",
      "article-title": "Ethyl substituted erythromycin derivatives produced by directed metabolic engineering",
      "volume": "95",
      "author": "Stassi",
      "year": "1998",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB24",
      "doi-asserted-by": "crossref",
      "first-page": "199",
      "DOI": "10.1126/science.279.5348.199",
      "article-title": "Engineering broader specificity into an antibiotic producing polyketide synthase",
      "volume": "279",
      "author": "Marsden",
      "year": "1998",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB25",
      "doi-asserted-by": "crossref",
      "first-page": "367",
      "DOI": "10.1126/science.277.5324.367",
      "article-title": "Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase",
      "volume": "277",
      "author": "Jacobsen",
      "year": "1997",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "1643",
      "DOI": "10.1021/bi9820311",
      "article-title": "Dissecting the role of acyltransferase domains of modular polyketide synthases in the choice and stereochemical fate of extender units",
      "volume": "38",
      "author": "Lau",
      "year": "1999",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "15464",
      "DOI": "10.1021/bi015864r",
      "article-title": "Alteration of the substrate specificity of a modular polyketide synthase acyltransferase domain through site-specific mutations",
      "volume": "40",
      "author": "Reeves",
      "year": "2001",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB28",
      "doi-asserted-by": "crossref",
      "first-page": "380",
      "DOI": "10.1039/a909079g",
      "article-title": "Polyketide biosynthesis: a millennium review",
      "volume": "18",
      "author": "Staunton",
      "year": "2001",
      "journal-title": "Nature Prod. Rep."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "63",
      "DOI": "10.1126/science.282.5386.63",
      "article-title": "Harnessing the biosynthetic code: combinations, permutations and mutations",
      "volume": "282",
      "author": "Cane",
      "year": "1998",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "491",
      "DOI": "10.1006/jmbi.2000.4272",
      "article-title": "The crystal structure of \u03b2-keto-acyl-carrier protein synthase II from Synechocystis sp at 1.54\u00c5 resolution and its relationship to other condensing enzymes",
      "volume": "305",
      "author": "Moche",
      "year": "2001",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "14808",
      "DOI": "10.1073/pnas.011399198",
      "article-title": "Crystal structure of the macrocycle forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel",
      "volume": "98",
      "author": "Tsai",
      "year": "2002",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB32",
      "doi-asserted-by": "crossref",
      "first-page": "12961",
      "DOI": "10.1074/jbc.270.22.12961",
      "article-title": "The E.coli malonyl-CoA: acyl carrier protein transacylase at 1.5\u00c5 resolution. Crystal structure of a FAS component",
      "volume": "270",
      "author": "Serre",
      "year": "1995",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "6000",
      "DOI": "10.1021/bi970006+",
      "article-title": "Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2)",
      "volume": "36",
      "author": "Crump",
      "year": "1997",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "281",
      "DOI": "10.1093/nar/30.1.281",
      "article-title": "CDD: a database of conserved domain alignments with links to domain three-dimensional structure",
      "volume": "30",
      "author": "Marchler-Bauer",
      "year": "2002",
      "journal-title": "Nucl. Acids Res."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB35",
      "doi-asserted-by": "crossref",
      "first-page": "7839",
      "DOI": "10.1073/pnas.92.17.7839",
      "article-title": "The biosynthetic gene cluster for the polyketide immunosuppresant rapamycin",
      "volume": "92",
      "author": "Schwecke",
      "year": "1995",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB36",
      "doi-asserted-by": "crossref",
      "first-page": "246",
      "DOI": "10.1016/0014-5793(95)01119-Y",
      "article-title": "Divergent sequence motifs correlated with the substrate specificity of (methyl) malonyl CoA:acyl carrier protein transacylase domains in modular polyketide synthases",
      "volume": "374",
      "author": "Haydock",
      "year": "1995",
      "journal-title": "FEBS Letters"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "9509",
      "DOI": "10.1073/pnas.96.17.9509",
      "article-title": "Organization of the biosynthetic gene cluster for the polyketide antihelmintic macrolide avermectin in Streptomyces avermitilis",
      "volume": "96",
      "author": "Ikeda",
      "year": "1999",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB38",
      "doi-asserted-by": "crossref",
      "first-page": "39",
      "DOI": "10.1111/j.1432-1033.1992.tb16603.x",
      "article-title": "6-Deoxyerythronolide-B synthase 2 from Saccharpolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme",
      "volume": "204",
      "author": "Bevitt",
      "year": "1992",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB39",
      "doi-asserted-by": "crossref",
      "first-page": "7515",
      "DOI": "10.1128/jb.179.23.7515-7522.1997",
      "article-title": "Identification and characterization of the niddamycin polyketide synthase genes from Streptomyces caelestis",
      "volume": "179",
      "author": "Kakavas",
      "year": "1997",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB40",
      "doi-asserted-by": "crossref",
      "first-page": "59",
      "DOI": "10.1016/S1074-5521(00)00056-9",
      "article-title": "Novel features in a combined polyketide synthase/non-ribosomal peptide synthetase: the myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sga15",
      "volume": "8",
      "author": "Silakowski",
      "year": "2001",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB41",
      "doi-asserted-by": "crossref",
      "first-page": "255",
      "DOI": "10.1016/S0378-1119(98)00338-2",
      "article-title": "Characterization of the enzymatic domains in the modular polyketide synthase involved in rifamycin B biosynthesis by Amycolatopsis mediterrannei",
      "volume": "216",
      "author": "Li",
      "year": "1998",
      "journal-title": "Gene"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB42",
      "doi-asserted-by": "crossref",
      "first-page": "395",
      "DOI": "10.1016/S1074-5521(00)00120-4",
      "article-title": "Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway",
      "volume": "7",
      "author": "Brautaset",
      "year": "2000",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB43",
      "doi-asserted-by": "crossref",
      "first-page": "51",
      "DOI": "10.1016/0378-1119(92)90602-L",
      "article-title": "Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea",
      "volume": "111",
      "author": "Donadio",
      "year": "1992",
      "journal-title": "Gene"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB44",
      "doi-asserted-by": "crossref",
      "first-page": "17",
      "DOI": "10.1016/S0378-1119(97)00501-5",
      "article-title": "Identification and sequence analysis of the genes encoding a polyketide synthase required for pyoluteorin biosynthesis in Pseudomonas fluorescens Pf-5",
      "volume": "204",
      "author": "Thompson",
      "year": "1997",
      "journal-title": "Gene"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB45",
      "doi-asserted-by": "crossref",
      "first-page": "37391",
      "DOI": "10.1074/jbc.274.52.37391",
      "article-title": "New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1",
      "volume": "274",
      "author": "Silakowski",
      "year": "1999",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB46",
      "doi-asserted-by": "crossref",
      "first-page": "797",
      "DOI": "10.1006/jmbi.1999.2583",
      "article-title": "GenTHREADER: an efficient and reliable protein fold recognition method for genomic sequences",
      "volume": "287",
      "author": "Jones",
      "year": "1999",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB47",
      "doi-asserted-by": "crossref",
      "first-page": "1215",
      "DOI": "10.1046/j.1365-2958.2002.02815.x",
      "article-title": "Engineering specificity of starter unit selection by the erythromycin-producing polyketide synthase",
      "volume": "43",
      "author": "Long",
      "year": "2002",
      "journal-title": "Mol. Microbiol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB48",
      "doi-asserted-by": "crossref",
      "first-page": "211",
      "DOI": "10.1016/S1074-5521(00)00091-0",
      "article-title": "Predictive, structure-based model of amino acid recognition by nonribosomal peptide synthetase adenylation domains",
      "volume": "7",
      "author": "Challis",
      "year": "2000",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB49",
      "doi-asserted-by": "crossref",
      "first-page": "11975",
      "DOI": "10.1074/jbc.272.18.11975",
      "article-title": "Alteration of substrate specificity of the malonyl CoA/acetyl CoA: acyl carrier protein s-acyltransferase domain of the multifunctional fatty acid synthase by a mutation of a single arginine residue",
      "volume": "272",
      "author": "Rangan",
      "year": "1997",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB50",
      "doi-asserted-by": "crossref",
      "first-page": "1192",
      "DOI": "10.1016/S1074-5521(01)00087-4",
      "article-title": "Chain initiation on the soraphen producing modular polyketide synthase from Sorangium cellulosum",
      "volume": "8",
      "author": "Wilkinson",
      "year": "2001",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB51",
      "doi-asserted-by": "crossref",
      "first-page": "3673",
      "DOI": "10.1128/jb.177.13.3673-3679.1995",
      "article-title": "A Sorangium cellulosum (myxobacterium) gene cluster for biosynthesis of macrolide antibiotic soraphen A: cloning, characterization, and homology to polyketide synthase genes from actinomycetes",
      "volume": "177",
      "author": "Schupp",
      "year": "1995",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB52",
      "doi-asserted-by": "crossref",
      "first-page": "13082",
      "DOI": "10.1074/jbc.M111738200",
      "article-title": "The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase",
      "volume": "277",
      "author": "Gaitatzis",
      "year": "2002",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB53",
      "doi-asserted-by": "crossref",
      "first-page": "65",
      "DOI": "10.1016/0378-1119(93)90347-6",
      "article-title": "A Bacillus subtilis large ORF coding for a polypeptide highly similar to polyketide synthases",
      "volume": "130",
      "author": "Scotti",
      "year": "1993",
      "journal-title": "Gene"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB54",
      "doi-asserted-by": "crossref",
      "first-page": "16741",
      "DOI": "10.1074/jbc.272.27.16741",
      "article-title": "Gene knock-out reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria",
      "volume": "272",
      "author": "Azad",
      "year": "1997",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB55",
      "doi-asserted-by": "crossref",
      "first-page": "5056",
      "DOI": "10.1021/bi012086u",
      "article-title": "Quantitative analysis of the relative contributions of donor acyl carrier proteins, acceptor ketosynthases, and linker regions to intermodular transfer of intermediates in hybrid polyketide synthases",
      "volume": "41",
      "author": "Wu",
      "year": "2002",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB56",
      "doi-asserted-by": "crossref",
      "first-page": "713",
      "DOI": "10.1016/S1074-5521(01)00046-1",
      "article-title": "Amphotericin biosynthesis in Sterptomyces nodosus: deductions from analysis of polyketide synthase and late genes",
      "volume": "8",
      "author": "Caffrey",
      "year": "2001",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB57",
      "doi-asserted-by": "crossref",
      "first-page": "81",
      "DOI": "10.1016/S0378-1119(00)00171-2",
      "article-title": "The Fk520 gene cluster of Streptomycetes hygroscopiocus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units",
      "volume": "251",
      "author": "Wu",
      "year": "2000",
      "journal-title": "Gene"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB58",
      "doi-asserted-by": "crossref",
      "first-page": "8524",
      "DOI": "10.1016/S0021-9258(17)37226-5",
      "article-title": "Limited Proteolysis and active-site studies of the first multienzyme component of the erythromycin-producing polyketide synthase",
      "volume": "269",
      "author": "Aparicio",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB59",
      "doi-asserted-by": "crossref",
      "first-page": "176",
      "DOI": "10.1038/348176a0",
      "article-title": "An unusually large multifuctional polypeptide in the erythromycin-producing PKS of Saccharopolyspora erythraea",
      "volume": "348",
      "author": "Cortes",
      "year": "1997",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB60",
      "doi-asserted-by": "crossref",
      "first-page": "97",
      "DOI": "10.1016/S1074-5521(00)00075-2",
      "article-title": "The biosynthetic gene cluster for the microtubule-stabilizing agents epothilones A and B from Sorangium cellulosum So ce90",
      "volume": "7",
      "author": "Molnar",
      "year": "2000",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB61",
      "doi-asserted-by": "crossref",
      "first-page": "752",
      "DOI": "10.1046/j.1365-2958.2000.02059.x",
      "article-title": "Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea",
      "volume": "37",
      "author": "Volchegursky",
      "year": "2000",
      "journal-title": "Mol. Microbiol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB62",
      "doi-asserted-by": "crossref",
      "first-page": "502",
      "DOI": "10.7164/antibiotics.53.502",
      "article-title": "Cloning, characterization and heterologous expression of a polyketide synthase and P-450 oxidase involved in the biosynthesis of the antibiotic oleandomycin",
      "volume": "53",
      "author": "Shah",
      "year": "2000",
      "journal-title": "J. Antibiot. (Tokyo)"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB63",
      "doi-asserted-by": "crossref",
      "first-page": "203",
      "DOI": "10.1016/S0378-1119(00)00003-2",
      "article-title": "Genetic architecture of the polyketide synthases for methymycin and pikromycin series macrolides",
      "volume": "245",
      "author": "Xue",
      "year": "2000",
      "journal-title": "Gene"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB64",
      "doi-asserted-by": "crossref",
      "first-page": "895",
      "DOI": "10.1016/S1074-5521(00)00038-7",
      "article-title": "A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis",
      "volume": "7",
      "author": "Aparicio",
      "year": "2000",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB65",
      "doi-asserted-by": "crossref",
      "first-page": "69",
      "DOI": "10.1016/S1074-5521(98)90141-7",
      "article-title": "Biosynthesis of the ansamycin antibiotic rifamycin, deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699",
      "volume": "5",
      "author": "August",
      "year": "1998",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB66",
      "doi-asserted-by": "crossref",
      "first-page": "487",
      "DOI": "10.1016/S1074-5521(01)00029-1",
      "article-title": "Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa",
      "volume": "8",
      "author": "Waldron",
      "year": "2001",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB67",
      "doi-asserted-by": "crossref",
      "first-page": "229",
      "DOI": "10.1023/A:1012065300116",
      "article-title": "The tylosin-biosynthetic genes of Streptomyces fradiae",
      "volume": "79",
      "author": "Cundliffe",
      "year": "2001",
      "journal-title": "Antonie Van Leeuwenhoek"
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB68",
      "doi-asserted-by": "crossref",
      "first-page": "13",
      "DOI": "10.1093/nar/30.1.13",
      "article-title": "Database resources of the National Center for Biotechnology Information: 2002 update",
      "volume": "30",
      "author": "Wheeler",
      "year": "2002",
      "journal-title": "Nucl. Acids Res."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB69",
      "doi-asserted-by": "crossref",
      "first-page": "403",
      "DOI": "10.1016/S0022-2836(05)80360-2",
      "article-title": "Basic local alignment search tool",
      "volume": "215",
      "author": "Altschul",
      "year": "1990",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/S0022-2836(03)00232-8_BIB70",
      "doi-asserted-by": "crossref",
      "first-page": "276",
      "DOI": "10.1093/nar/30.1.276",
      "article-title": "The Pfam Protein Families Database",
      "volume": "30",
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