DOI: 10.1016/s0022-2275(20)40580-2. Improved methods for the assay and activation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.

Improved methods for the assay and activation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.

10.1016/s0022-2275(20)40580-2

Crossref journal-article

Elsevier BV

Journal of Lipid Research (78)

Bibliography

Philipp, B. W., & Shapiro, D. J. (1979). Improved methods for the assay and activation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Journal of Lipid Research, 20(5), 588â593.

Authors 2

B W Philipp (first)
D J Shapiro (additional)

References 20 Referenced 120

10.1016/B978-0-12-024914-5.50008-5 / Adv. Lipid Res. / Regulation of HMG-CoA reductase by Rodwell (1976)
{'key': '10.1016/S0022-2275(20)40580-2_bib2', 'first-page': '373', 'article-title': 'Regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase', 'volume': '38', 'author': 'Rodwell', 'year': '1973', 'journal-title': 'Adv. Enzymol.'} / Adv. Enzymol. / Regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase by Rodwell (1973)
10.1038/newbio246060a0 / Nature (London) New Biol. / Regulation of rat liver β-hydroxy-β-methylglutaryl-CoA reductase activity by cholesterol by Higgins (1973)
10.1016/0003-2697(69)90279-6 / Anal. Biochem. / Thin-layer chromatographic assay for HMG-CoA reductase and mevalonic acid by Shapiro (1969)
10.1016/0005-2744(74)90098-9 / Biochim. Biophys. Acta. / Microassay for 3-hydroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts by Shapiro (1974)
10.1016/S0022-2275(20)39504-3 / J. Lipid Res. / Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase by Goldfarb (1971)
{'key': '10.1016/S0022-2275(20)40580-2_bib7', 'first-page': '1645', 'article-title': 'A simple and rapid radiochemical assay for 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Hoppe-Seyler’s Z', 'volume': '354', 'author': 'Huber', 'year': '1973', 'journal-title': 'Physiol. Chem.'} / Physiol. Chem. / A simple and rapid radiochemical assay for 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Hoppe-Seyler’s Z by Huber (1973)
10.1016/0003-2697(77)90599-1 / Anal. Biochem. / A simplified procedure for the concentration, desalting and thin-layer chromatography of mevalonic acid from reaction mixtures used to assay 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity by Murthy (1977)
10.1042/bj1640501 / Biochem. J. / A new method for assaying rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and its application in a study of the effect of dietary cholesterol on this enzyme by Baqir (1977)
10.1021/bi00815a020 / Biochemistry. / Biosynthesis of 3-hydroxy-3-methylglutarate and mevalonate by rat liver homogenates in vitro by White (1970)
10.1016/S0022-2275(20)41692-X / J. Lipid Res. / The quantitative assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase: comparison of a thin-layer chromatographic assay with a rapid chloroform extraction assay by Ackermann (1977)
10.1016/S0022-2275(20)36843-7 / J. Lipid Res. / Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase by measurement of coenzyme A by Hulcher (1973)
10.1016/S0021-9258(19)41681-5 / J. Biol. Chem. / Abnormal induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase in leukocytes from subjects with heterozygous familial hypercholesterolemia by Fogelman (1975)
10.1016/S0022-2275(20)36991-1 / J. Lipid Res. / Improved methods for the study of hepatic HMG-CoA reductase: one-step isolation of mevalonolactone and rapid preparation of endoplasmic reticulum by Goodwin (1976)
10.1016/S0021-9258(17)38328-X / J. Biol. Chem. / Interconversion of active and inactive forms of rat liver hydroxymethylglutaryl-CoA reductase by Nordstrom (1977)
10.1016/0006-291X(73)91137-6 / Biochem. Biophys. Res. Commun. / Modulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity with cAMP and with protein fractions of rat liver cytosol by Beg (1973)
10.1016/0006-291X(78)91273-1 / Biochem. Biophys. Res. Commun. / Reversible modulation of the activities of both liver microsomal hydroxymethylglutaryl coenzyme A reductase and its inactivating enzyme. Evidence for the regulation by phosphorylation–dephosphorylation by Ingebritsen (1978)
10.1016/S0021-9258(18)62216-1 / J. Biol. Chem. / Regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol synthesis by Shapiro (1971)
{'key': '10.1016/S0022-2275(20)40580-2_bib19', 'first-page': '468', 'article-title': 'Removal of phosphate groups from casein with potato acid phosphatase', 'volume': '71', 'author': 'Bingham', 'year': '1976', 'journal-title': 'Biochim. Biophys. Acta.'} / Biochim. Biophys. Acta. / Removal of phosphate groups from casein with potato acid phosphatase by Bingham (1976)
10.1016/S0021-9258(19)52451-6 / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry (1951)

Dates

Type	When
Created	4 years, 7 months ago (Jan. 7, 2021, 12:38 p.m.)
Deposited	3 years, 9 months ago (Nov. 20, 2021, 4:46 p.m.)
Indexed	11 minutes ago (Sept. 4, 2025, 4:18 a.m.)
Issued	46 years, 2 months ago (July 1, 1979)
Published	46 years, 2 months ago (July 1, 1979)
Published Print	46 years, 2 months ago (July 1, 1979)

Funders 0

None

BibTeX

@article{Philipp_1979, title={Improved methods for the assay and activation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.}, volume={20}, ISSN={0022-2275}, url={http://dx.doi.org/10.1016/s0022-2275(20)40580-2}, DOI={10.1016/s0022-2275(20)40580-2}, number={5}, journal={Journal of Lipid Research}, publisher={Elsevier BV}, author={Philipp, B W and Shapiro, D J}, year={1979}, month=jul, pages={588–593} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T08:18:43Z",
    "timestamp": 1756973923975
  },
  "reference-count": 20,
  "publisher": "Elsevier BV",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1979,
            7,
            1
          ]
        ],
        "date-time": "1979-07-01T00:00:00Z",
        "timestamp": 299635200000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2020,
            9,
            29
          ]
        ],
        "date-time": "2020-09-29T00:00:00Z",
        "timestamp": 1601337600000
      },
      "content-version": "vor",
      "delay-in-days": 15066,
      "URL": "http://creativecommons.org/licenses/by/4.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1979,
        7
      ]
    ]
  },
  "DOI": "10.1016/s0022-2275(20)40580-2",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2021,
        1,
        7
      ]
    ],
    "date-time": "2021-01-07T17:38:06Z",
    "timestamp": 1610041086000
  },
  "page": "588-593",
  "source": "Crossref",
  "is-referenced-by-count": 120,
  "title": "Improved methods for the assay and activation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.",
  "prefix": "10.1016",
  "volume": "20",
  "author": [
    {
      "given": "B W",
      "family": "Philipp",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "D J",
      "family": "Shapiro",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1016/B978-0-12-024914-5.50008-5",
      "article-title": "Regulation of HMG-CoA reductase",
      "volume": "14",
      "author": "Rodwell",
      "year": "1976",
      "journal-title": "Adv. Lipid Res."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib2",
      "first-page": "373",
      "article-title": "Regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase",
      "volume": "38",
      "author": "Rodwell",
      "year": "1973",
      "journal-title": "Adv. Enzymol."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "60",
      "DOI": "10.1038/newbio246060a0",
      "article-title": "Regulation of rat liver \u03b2-hydroxy-\u03b2-methylglutaryl-CoA reductase activity by cholesterol",
      "volume": "246",
      "author": "Higgins",
      "year": "1973",
      "journal-title": "Nature (London) New Biol."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "383",
      "DOI": "10.1016/0003-2697(69)90279-6",
      "article-title": "Thin-layer chromatographic assay for HMG-CoA reductase and mevalonic acid",
      "volume": "31",
      "author": "Shapiro",
      "year": "1969",
      "journal-title": "Anal. Biochem."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "369",
      "DOI": "10.1016/0005-2744(74)90098-9",
      "article-title": "Microassay for 3-hydroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts",
      "volume": "370",
      "author": "Shapiro",
      "year": "1974",
      "journal-title": "Biochim. Biophys. Acta."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "512",
      "DOI": "10.1016/S0022-2275(20)39504-3",
      "article-title": "Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase",
      "volume": "12",
      "author": "Goldfarb",
      "year": "1971",
      "journal-title": "J. Lipid Res."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib7",
      "first-page": "1645",
      "article-title": "A simple and rapid radiochemical assay for 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Hoppe-Seyler\u2019s Z",
      "volume": "354",
      "author": "Huber",
      "year": "1973",
      "journal-title": "Physiol. Chem."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "65",
      "DOI": "10.1016/0003-2697(77)90599-1",
      "article-title": "A simplified procedure for the concentration, desalting and thin-layer chromatography of mevalonic acid from reaction mixtures used to assay 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity",
      "volume": "81",
      "author": "Murthy",
      "year": "1977",
      "journal-title": "Anal. Biochem."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "501",
      "DOI": "10.1042/bj1640501",
      "article-title": "A new method for assaying rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and its application in a study of the effect of dietary cholesterol on this enzyme",
      "volume": "164",
      "author": "Baqir",
      "year": "1977",
      "journal-title": "Biochem. J."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "2713",
      "DOI": "10.1021/bi00815a020",
      "article-title": "Biosynthesis of 3-hydroxy-3-methylglutarate and mevalonate by rat liver homogenates in vitro",
      "volume": "9",
      "author": "White",
      "year": "1970",
      "journal-title": "Biochemistry."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "408",
      "DOI": "10.1016/S0022-2275(20)41692-X",
      "article-title": "The quantitative assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase: comparison of a thin-layer chromatographic assay with a rapid chloroform extraction assay",
      "volume": "18",
      "author": "Ackermann",
      "year": "1977",
      "journal-title": "J. Lipid Res."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "625",
      "DOI": "10.1016/S0022-2275(20)36843-7",
      "article-title": "Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase by measurement of coenzyme A",
      "volume": "14",
      "author": "Hulcher",
      "year": "1973",
      "journal-title": "J. Lipid Res."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "2045",
      "DOI": "10.1016/S0021-9258(19)41681-5",
      "article-title": "Abnormal induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase in leukocytes from subjects with heterozygous familial hypercholesterolemia",
      "volume": "250",
      "author": "Fogelman",
      "year": "1975",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "297",
      "DOI": "10.1016/S0022-2275(20)36991-1",
      "article-title": "Improved methods for the study of hepatic HMG-CoA reductase: one-step isolation of mevalonolactone and rapid preparation of endoplasmic reticulum",
      "volume": "17",
      "author": "Goodwin",
      "year": "1976",
      "journal-title": "J. Lipid Res."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "8924",
      "DOI": "10.1016/S0021-9258(17)38328-X",
      "article-title": "Interconversion of active and inactive forms of rat liver hydroxymethylglutaryl-CoA reductase",
      "volume": "252",
      "author": "Nordstrom",
      "year": "1977",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "1362",
      "DOI": "10.1016/0006-291X(73)91137-6",
      "article-title": "Modulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity with cAMP and with protein fractions of rat liver cytosol",
      "volume": "54",
      "author": "Beg",
      "year": "1973",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "1268",
      "DOI": "10.1016/0006-291X(78)91273-1",
      "article-title": "Reversible modulation of the activities of both liver microsomal hydroxymethylglutaryl coenzyme A reductase and its inactivating enzyme. Evidence for the regulation by phosphorylation\u2013dephosphorylation",
      "volume": "81",
      "author": "Ingebritsen",
      "year": "1978",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "3210",
      "DOI": "10.1016/S0021-9258(18)62216-1",
      "article-title": "Regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol synthesis",
      "volume": "246",
      "author": "Shapiro",
      "year": "1971",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib19",
      "first-page": "468",
      "article-title": "Removal of phosphate groups from casein with potato acid phosphatase",
      "volume": "71",
      "author": "Bingham",
      "year": "1976",
      "journal-title": "Biochim. Biophys. Acta."
    },
    {
      "key": "10.1016/S0022-2275(20)40580-2_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "265",
      "DOI": "10.1016/S0021-9258(19)52451-6",
      "article-title": "Protein measurement with the Folin phenol reagent",
      "volume": "193",
      "author": "Lowry",
      "year": "1951",
      "journal-title": "J. Biol. Chem."
    }
  ],
  "container-title": "Journal of Lipid Research",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0022227520405802?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0022227520405802?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        20
      ]
    ],
    "date-time": "2021-11-20T21:46:24Z",
    "timestamp": 1637444784000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S0022227520405802"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1979,
        7
      ]
    ]
  },
  "references-count": 20,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1979,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "S0022227520405802"
  ],
  "URL": "http://dx.doi.org/10.1016/S0022-2275(20)40580-2",
  "relation": {},
  "ISSN": [
    "0022-2275"
  ],
  "subject": [],
  "container-title-short": "Journal of Lipid Research",
  "published": {
    "date-parts": [
      [
        1979,
        7
      ]
    ]
  }
}