DOI: 10.1016/s0014-5793(99)01566-5. Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation

Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation

10.1016/s0014-5793(99)01566-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

To investigate the possible influence of the local rates of translation on protein folding, 16 consecutive rare (in Escherichia coli) codons in the chloramphenicol acetyltransferase (CAT) gene have been replaced by frequent ones. Site‐directed silent mutagenesis reduced the pauses in translation of CAT in E. coli S30 extract cell‐free system and led to the acceleration of the overall rate of CAT protein synthesis. At the same time, the silently mutated protein (with unaltered protein sequence) synthesized in the E. coli S30 extract system was shown to possess 20% lower specific activity. The data suggest that kinetics of protein translation can affect the in vivo protein‐folding pathway, leading to increased levels of protein misfolding.

Bibliography

Komar, A. A., Lesnik, T., & Reiss, C. (1999). Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation. FEBS Letters, 462(3), 387â391. Portico.

Authors 3

Anton A Komar (first)
Thierry Lesnik (additional)
Claude Reiss (additional)

References 62 Referenced 329

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:52 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:58 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 3:46 p.m.)
Issued	25 years, 9 months ago (Nov. 30, 1999)
Published	25 years, 9 months ago (Nov. 30, 1999)
Published Online	25 years, 9 months ago (Nov. 30, 1999)
Published Print	25 years, 8 months ago (Dec. 3, 1999)

Funders 0

None

BibTeX

@article{Komar_1999, title={Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation}, volume={462}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)01566-5}, DOI={10.1016/s0014-5793(99)01566-5}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Komar, Anton A and Lesnik, Thierry and Reiss, Claude}, year={1999}, month=nov, pages={387–391} }

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