DOI: 10.1016/s0014-5793(99)01518-5. Voltage‐dependent Ca<sup>2+</sup> channel subunit expression and immunolocalization in mouse spermatogenic cells and sperm

Voltage‐dependent Ca2+ channel subunit expression and immunolocalization in mouse spermatogenic cells and sperm

10.1016/s0014-5793(99)01518-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Though voltage‐dependent Ca2+ channels contribute to the orchestratation of sperm differentiation and function, many questions remain concerning their molecular architecture. This study shows that α1A and α1C Ca2+ channel pore‐forming subunits are expressed in spermatogenic cells. In addition, it provides what is to our knowledge the first evidence for the presence of the Ca2+ channel β auxiliary subunits in spermatogenic cells and sperm. Using RT‐PCR we demonstrated the expression of all four known genes encoding the β subunits in spermatogenic cells. Specific antibodies detected three of these proteins in spermatogenic cells and sperm. In spermatogenic cells both α1 and β subunits are diffusely distributed throughout the cytoplasm while in sperm they appear to be regionally localized.

Bibliography

Serrano, C. J., TreviÃ±o, C. L., Felix, R., & Darszon, A. (1999). Voltageâdependent Ca2+ channel subunit expression and immunolocalization in mouse spermatogenic cells and sperm. FEBS Letters, 462(1â2), 171â176. Portico.

Authors 4

Carmen J Serrano (first)
Claudia L Treviño (additional)
Ricardo Felix (additional)
Alberto Darszon (additional)

References 26 Referenced 56

10.1152/physrev.1999.79.2.481
10.2741/A358
De Waard M. Gurnett C.A. and Campbell K.P. (1996) in: Ion Channels Vol. IV (Narahashi T. Ed.) 41–87 Plenum Press New York. (10.1007/978-1-4899-1775-1_2)
10.1016/S0166-2236(97)01200-9
10.1038/36110
10.1161/01.RES.83.1.103
10.1523/JNEUROSCI.18-21-08605.1998
Liu H. and Campbell K.P. (1998) in: Low-Voltage-Activated T-Type Calcium Channels (Tsien R.W. Clozel J.-P. and Nargeot J. Eds.) 229–243 Proceedings from the International Electrophysiology Meeting Montpellier.
10.1016/S0021-9258(18)48135-5
10.1016/0012-1606(92)90137-6
10.1016/S0014-5793(98)00305-6
10.1113/jphysiol.1984.sp015457
10.1152/ajpcell.1996.271.5.C1583
10.1073/pnas.93.23.13004
10.1016/0014-5793(96)00515-7
10.1093/molehr/3.3.255
10.1002/(SICI)1520-6408(1999)25:2<103::AID-DVG4>3.0.CO;2-B
10.1093/molehr/4.3.215
10.1006/dbio.1998.9172
10.1074/jbc.271.23.13804
10.1074/jbc.271.6.3207
10.1074/jbc.272.29.18508
10.1016/S0074-7696(08)60766-4
10.1085/jgp.112.1.33
10.1016/S0896-6273(00)80461-4
10.1111/j.1469-7793.1999.0035o.x

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:52 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 10:58 p.m.)
Indexed	5 months, 1 week ago (March 19, 2025, 9:20 a.m.)
Issued	25 years, 9 months ago (Nov. 22, 1999)
Published	25 years, 9 months ago (Nov. 22, 1999)
Published Online	25 years, 9 months ago (Nov. 22, 1999)
Published Print	25 years, 9 months ago (Nov. 26, 1999)

Funders 0

None

BibTeX

@article{Serrano_1999, title={Voltage‐dependent Ca2+ channel subunit expression and immunolocalization in mouse spermatogenic cells and sperm}, volume={462}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)01518-5}, DOI={10.1016/s0014-5793(99)01518-5}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Serrano, Carmen J and Treviño, Claudia L and Felix, Ricardo and Darszon, Alberto}, year={1999}, month=nov, pages={171–176} }

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