DOI: 10.1016/s0014-5793(99)01182-5. Development of a quantitative gene expression assay for <i>Chlamydia trachomatis</i> identified temporal expression of σ factors

Development of a quantitative gene expression assay for Chlamydia trachomatis identified temporal expression of σ factors

10.1016/s0014-5793(99)01182-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Chlamydia trachomatis is an important human pathogen which possesses a unique bi‐phasic developmental cycle. We used lightcycler methodology to quantitatively measure gene transcript levels in C. trachomatis strain L2. By measuring 16S rRNA transcript levels, we determined C. trachomatis L2 to have a generation time of approximately 3 h and an inclusion burst size of 200–300 particles. The three chlamydial σ factor genes rpoD (σ66), rpsD (σ28) and rpoN (σ54) exhibited different patterns of temporal expression. rpoD was central to early chlamydial development, whereas rpsD and rpoN were temporally expressed, coinciding with elementary body (EB) to reticulate body (RB) conversion and RB to EB conversion, respectively.

Authors 3

Sarah A Mathews (first)
Kym M Volp (additional)
Peter Timms (additional)

References 27 Referenced 68

Storz J. (1988) in: Microbiology of Chlamydia (Barron A.L. Ed.) pp. 167–192 CRC Press.
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10.1128/jb.170.2.744-750.1988
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10.1111/j.1365-2958.1991.tb01831.x
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10.1128/jb.177.4.877-882.1995

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:52 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 12:20 p.m.)
Indexed	3 months, 1 week ago (May 24, 2025, 3:26 a.m.)
Issued	25 years, 11 months ago (Sept. 21, 1999)
Published	25 years, 11 months ago (Sept. 21, 1999)
Published Online	25 years, 11 months ago (Sept. 21, 1999)
Published Print	25 years, 11 months ago (Sept. 24, 1999)

Funders 0

None

BibTeX

@article{Mathews_1999, title={Development of a quantitative gene expression assay for Chlamydia trachomatis identified temporal expression of σ factors}, volume={458}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)01182-5}, DOI={10.1016/s0014-5793(99)01182-5}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Mathews, Sarah A and Volp, Kym M and Timms, Peter}, year={1999}, month=sep, pages={354–358} }

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