DOI: 10.1016/s0014-5793(99)01117-5. The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE δ, interacts with the Arf‐like protein Arl3 in a GTP specific manner

The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE δ, interacts with the Arf‐like protein Arl3 in a GTP specific manner

10.1016/s0014-5793(99)01117-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Recently, we have shown that the δ subunit of the cGMP phosphodiesterase (PDE δ) interacts with the retinitis pigmentosa guanine regulator (RPGR). Here, using the two‐hybrid system, we identify a member of the Arf‐like protein family of Ras‐related GTP‐binding proteins, Arl3, that interacts with PDE δ. The interaction was verified by fluorescence spectroscopy and co‐immunoprecipitation. Arl3 features an unusually low affinity for guanine nucleotides, with a K D of 24 nM for GDP and 48 μM for GTP. Fluorescence spectroscopy shows that PDE δ binds and specifically stabilizes the GTP‐bound form of Arl3 by strongly decreasing the dissociation rate of GTP. Thus, PDE δ is an effector of Arl3 and could provide a novel nucleotide exchange mechanism by which PDE δ stabilizes Arl3 in its active GTP‐bound form.

Bibliography

Linari, M., Hanzal-Bayer, M., & Becker, J. (1999). The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE Î´, interacts with the Arfâlike protein Arl3 in a GTP specific manner. FEBS Letters, 458(1), 55â59. Portico.

Authors 3

Marco Linari (first)
Michael Hanzal-Bayer (additional)
Jörg Becker (additional)

References 21 Referenced 83

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:52 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 7:38 a.m.)
Indexed	2 months, 1 week ago (June 20, 2025, 12:06 p.m.)
Issued	25 years, 11 months ago (Sept. 3, 1999)
Published	25 years, 11 months ago (Sept. 3, 1999)
Published Online	25 years, 11 months ago (Sept. 3, 1999)
Published Print	25 years, 11 months ago (Sept. 10, 1999)

Funders 0

None

BibTeX

@article{Linari_1999, title={The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE δ, interacts with the Arf‐like protein Arl3 in a GTP specific manner}, volume={458}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)01117-5}, DOI={10.1016/s0014-5793(99)01117-5}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Linari, Marco and Hanzal-Bayer, Michael and Becker, Jörg}, year={1999}, month=sep, pages={55–59} }

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