DOI: 10.1016/s0014-5793(99)00768-1. Mutational analysis of subunit iβ2 (MECL‐1) demonstrates conservation of cleavage specificity between yeast and mammalian proteasomes

Mutational analysis of subunit iβ2 (MECL‐1) demonstrates conservation of cleavage specificity between yeast and mammalian proteasomes

10.1016/s0014-5793(99)00768-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Proteasomes are the major protein‐degrading complexes in the cytosol and regulate many cellular processes. To examine the functional importance of the MC14/MECL‐1 proteasome active site subunits, cell lines expressing a catalytically inactive form of MECL‐1 were established. Whereas mutant MECL‐1 was readily incorporated into cytosolic proteasomes, replacing the constitutive MC14 subunit, removal of the prosequence was incomplete indicating that its processing required autocatalytic cleavage. Functional analyses showed that the absence of the MC14/MECL‐1 active sites abrogated proteasomal trypsin‐like activity, but did not affect other catalytic activities. Our data demonstrate a conservation of cleavage specificity between mammalian and yeast proteasomes.

Bibliography

Salzmann, U., Kral, S., Braun, B., Standera, S., Schmidt, M., Kloetzel, P.-M., & Sijts, A. (1999). Mutational analysis of subunit iÎ²2 (MECLâ1) demonstrates conservation of cleavage specificity between yeast and mammalian proteasomes. FEBS Letters, 454(1â2), 11â15. Portico.

Authors 7

Ulrike Salzmann (first)
Sylvie Kral (additional)
Beate Braun (additional)
Sybille Standera (additional)
Marion Schmidt (additional)
Peter-M Kloetzel (additional)
Alice Sijts (additional)

References 27 Referenced 25

10.1046/j.1365-2443.1998.00207.x
10.1146/annurev.biochem.67.1.425
10.1038/386463a0
10.1074/jbc.271.21.12150
10.1016/S0092-8674(00)80097-5
10.1038/381166a0
10.1042/bj3320749
10.1096/fasebj.11.14.9409542
10.1016/0167-5699(96)10051-7
10.1084/jem.187.1.97
10.1084/jem.179.3.901
10.1074/jbc.270.40.23808
10.1038/365264a0
10.1073/pnas.92.2.584
10.1074/jbc.272.18.11824
10.1084/jem.187.10.1641
10.1074/jbc.272.40.25200
10.1074/jbc.273.40.25637
10.1073/pnas.95.21.12504
10.1002/eji.1830260421
10.4049/jimmunol.156.7.2361
10.1084/jem.183.4.1807
10.1073/pnas.94.17.8970
10.1002/eji.1830250930
10.1002/j.1460-2075.1996.tb01081.x
Schmidt M. Zantopf D. Dick C. Kraft R. Kostka S. Preißner R. Frömmel C. and Kloetzel P.-M. (1999) J. Mol. Biol. (in press).
10.1093/emboj/16.20.6087

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 8:18 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 8:39 a.m.)
Indexed	4 months, 4 weeks ago (March 31, 2025, 4:42 a.m.)
Issued	26 years, 1 month ago (July 2, 1999)
Published	26 years, 1 month ago (July 2, 1999)
Published Online	26 years, 1 month ago (July 6, 1999)
Published Print	26 years, 1 month ago (July 2, 1999)

Funders 0

None

BibTeX

@article{Salzmann_1999, title={Mutational analysis of subunit iβ2 (MECL‐1) demonstrates conservation of cleavage specificity between yeast and mammalian proteasomes}, volume={454}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)00768-1}, DOI={10.1016/s0014-5793(99)00768-1}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Salzmann, Ulrike and Kral, Sylvie and Braun, Beate and Standera, Sybille and Schmidt, Marion and Kloetzel, Peter-M and Sijts, Alice}, year={1999}, month=jul, pages={11–15} }

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