DOI: 10.1016/s0014-5793(99)00730-9. A novel substrate for analyzing Alzheimer's disease γ‐secretase

A novel substrate for analyzing Alzheimer's disease γ‐secretase

10.1016/s0014-5793(99)00730-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Proteolytic processing of Alzheimer's disease amyloid precursor protein (APP) by β‐secretase leads to A4CT (C99), which is further cleaved by the as yet unknown protease called γ‐secretase. To study the enzymatic properties of γ‐secretase independently of β‐secretase, A4CT together with an N‐terminal signal peptide (SPA4CT) may be expressed in eukaryotic cells. However, in all existing SPA4CT proteins the signal peptide is not correctly cleaved upon membrane insertion. Here, we report the generation of a mutated SPA4CT protein that is correctly cleaved by signal peptidase and, thus, identical to the APP‐derived A4CT. This novel SPA4CT protein is processed by γ‐secretase in the same manner as APP‐derived A4CT and might be valuable for the generation of transgenic animals showing amyloid pathology.

Bibliography

Lichtenthaler, S. F., Multhaup, G., Masters, C. L., & Beyreuther, K. (1999). A novel substrate for analyzing Alzheimerâs disease Î³âsecretase. FEBS Letters, 453(3), 288â292. Portico.

Authors 4

Stefan F. Lichtenthaler (first)
Gerd Multhaup (additional)
Colin L. Masters (additional)
Konrad Beyreuther (additional)

References 33 Referenced 42

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:52 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 11:02 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 16, 2024, 4:01 p.m.)
Issued	26 years, 2 months ago (June 23, 1999)
Published	26 years, 2 months ago (June 23, 1999)
Published Online	26 years, 2 months ago (June 23, 1999)
Published Print	26 years, 2 months ago (June 25, 1999)

Funders 0

None

BibTeX

@article{Lichtenthaler_1999, title={A novel substrate for analyzing Alzheimer’s disease γ‐secretase}, volume={453}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)00730-9}, DOI={10.1016/s0014-5793(99)00730-9}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Lichtenthaler, Stefan F. and Multhaup, Gerd and Masters, Colin L. and Beyreuther, Konrad}, year={1999}, month=jun, pages={288–292} }

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