DOI: 10.1016/s0014-5793(99)00565-7. Isolation of reductase for SoxR that governs an oxidative response regulon from <i>Escherichia coli</i>

Isolation of reductase for SoxR that governs an oxidative response regulon from Escherichia coli

10.1016/s0014-5793(99)00565-7

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

SoxR is a transcription factor triggered by oxidative stress in Escherichia coli. Recent evidence suggests that novel redox regulation couples oxidation state to promoter activation. We have isolated the reductase for SoxR in E. coli using an assay of NADPH‐ and SoxR‐dependent cytochrome c reductase activity. When the purified protein was incubated in an anaerobic reaction mixture containing SoxR and NADPH, the reduction of [2Fe‐2S] cluster of SoxR was observed by optical and EPR spectroscopy. Our results indicate that the purified protein serves as an NADPH‐dependent reduction system for SoxR.

Bibliography

Kobayashi, K., & Tagawa, S. (1999). Isolation of reductase for SoxR that governs an oxidative response regulon from Escherichia coli. FEBS Letters, 451(3), 227â230. Portico.

Authors 2

Kazuo Kobayashi (first)
Seiichi Tagawa (additional)

References 20 Referenced 42

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 11:03 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 2:08 p.m.)
Indexed	1 year, 7 months ago (Jan. 18, 2024, 12:27 a.m.)
Issued	26 years, 3 months ago (May 28, 1999)
Published	26 years, 3 months ago (May 28, 1999)
Published Online	26 years, 2 months ago (June 2, 1999)
Published Print	26 years, 3 months ago (May 28, 1999)

Funders 0

None

BibTeX

@article{Kobayashi_1999, title={Isolation of reductase for SoxR that governs an oxidative response regulon from Escherichia coli}, volume={451}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)00565-7}, DOI={10.1016/s0014-5793(99)00565-7}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Kobayashi, Kazuo and Tagawa, Seiichi}, year={1999}, month=may, pages={227–230} }

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