DOI: 10.1016/s0014-5793(99)00516-5. Identification and characterization of GABA, proline and quaternary ammonium compound transporters from <i>Arabidopsis thaliana</i>

Identification and characterization of GABA, proline and quaternary ammonium compound transporters from Arabidopsis thaliana

10.1016/s0014-5793(99)00516-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Arabidopsis thaliana grows efficiently on GABA as the sole nitrogen source, thereby providing evidence for the existence of GABA transporters in plants. Heterologous complementation of a GABA uptake‐deficient yeast mutant identified two previously known plant amino acid transporters, AAP3 and ProT2, as GABA transporters with Michaelis constants of 12.9±1.7 and 1.7±0.3 mM at pH 4, respectively. The simultaneous transport of [1‐14C]GABA and [2,3‐3H]proline by ProT2 as a function of pH, provided evidence that the zwitterionic state of GABA is an important parameter in substrate recognition. ProT2‐mediated [1‐14C]GABA transport was inhibited by proline and quaternary ammonium compounds.

Bibliography

Breitkreuz, K. E., Shelp, B. J., Fischer, W. N., Schwacke, R., & Rentsch, D. (1999). Identification and characterization of GABA, proline and quaternary ammonium compound transporters from Arabidopsis thaliana. FEBS Letters, 450(3), 280â284. Portico.

Authors 5

Kevin E. Breitkreuz (first)
Barry J. Shelp (additional)
Wolf N. Fischer (additional)
Rainer Schwacke (additional)
Doris Rentsch (additional)

References 40 Referenced 112

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:16 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 7:49 p.m.)
Indexed	1 week, 1 day ago (Aug. 21, 2025, 1:57 p.m.)
Issued	26 years, 3 months ago (May 7, 1999)
Published	26 years, 3 months ago (May 7, 1999)
Published Online	26 years, 1 month ago (July 13, 1999)
Published Print	26 years, 3 months ago (May 7, 1999)

Funders 0

None

BibTeX

@article{Breitkreuz_1999, title={Identification and characterization of GABA, proline and quaternary ammonium compound transporters from Arabidopsis thaliana}, volume={450}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)00516-5}, DOI={10.1016/s0014-5793(99)00516-5}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Breitkreuz, Kevin E. and Shelp, Barry J. and Fischer, Wolf N. and Schwacke, Rainer and Rentsch, Doris}, year={1999}, month=may, pages={280–284} }

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