DOI: 10.1016/s0014-5793(99)00432-9. μ1B, a novel adaptor medium chain expressed in polarized epithelial cells<sup>1</sup>

μ1B, a novel adaptor medium chain expressed in polarized epithelial cells1

10.1016/s0014-5793(99)00432-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The apical and basolateral plasma membrane domains of polarized epithelial cells contain distinct sets of integral membrane proteins. Biosynthetic targeting of proteins to the basolateral plasma membrane is mediated by cytosolic tail determinants, many of which resemble signals involved in the rapid endocytosis or lysosomal targeting. Since these signals are recognized by adaptor proteins, we hypothesized that there could be epithelial‐specific adaptors involved in polarized sorting. Here, we report the identification of a novel member of the adaptor medium chain family, named μ1B, which is closely related to the previously described μ1A (79% amino acid sequence identity). Northern blotting and in situ hybridization analyses reveal the specific expression of μ1B mRNA in a subset of polarized epithelial and exocrine cells. Yeast two‐hybrid analyses show that μ1B is capable of interacting with generic tyrosine‐based sorting signals. These observations suggest that μ1B may be involved in protein sorting events specific to polarized cells.

Bibliography

Ohno, H., Tomemori, T., Nakatsu, F., Okazaki, Y., Aguilar, R. C., Foelsch, H., Mellman, I., Saito, T., Shirasawa, T., & Bonifacino, J. S. (1999). Î¼1B, a novel adaptor medium chain expressed in polarized epithelial cells1. FEBS Letters, 449(2â3), 215â220. Portico.

Authors 10

Hiroshi Ohno (first)
Takuya Tomemori (additional)
Fubito Nakatsu (additional)
Yasushi Okazaki (additional)
Ruben C. Aguilar (additional)
Heike Foelsch (additional)
Ira Mellman (additional)
Takashi Saito (additional)
Takuji Shirasawa (additional)
Juan S. Bonifacino (additional)

References 42 Referenced 221

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 8:18 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 1:14 p.m.)
Indexed	4 days, 23 hours ago (Aug. 23, 2025, 12:58 a.m.)
Issued	26 years, 4 months ago (April 23, 1999)
Published	26 years, 4 months ago (April 23, 1999)
Published Online	26 years, 4 months ago (April 23, 1999)
Published Print	26 years, 4 months ago (April 23, 1999)

Funders 0

None

BibTeX

@article{Ohno_1999, title={μ1B, a novel adaptor medium chain expressed in polarized epithelial cells1}, volume={449}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)00432-9}, DOI={10.1016/s0014-5793(99)00432-9}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Ohno, Hiroshi and Tomemori, Takuya and Nakatsu, Fubito and Okazaki, Yasushi and Aguilar, Ruben C. and Foelsch, Heike and Mellman, Ira and Saito, Takashi and Shirasawa, Takuji and Bonifacino, Juan S.}, year={1999}, month=apr, pages={215–220} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        23
      ]
    ],
    "date-time": "2025-08-23T04:58:12Z",
    "timestamp": 1755925092724
  },
  "reference-count": 42,
  "publisher": "Wiley",
  "issue": "2-3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1999,
            4,
            23
          ]
        ],
        "date-time": "1999-04-23T00:00:00Z",
        "timestamp": 924825600000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1999,
        4,
        23
      ]
    ]
  },
  "abstract": "<jats:p>The apical and basolateral plasma membrane domains of polarized epithelial cells contain distinct sets of integral membrane proteins. Biosynthetic targeting of proteins to the basolateral plasma membrane is mediated by cytosolic tail determinants, many of which resemble signals involved in the rapid endocytosis or lysosomal targeting. Since these signals are recognized by adaptor proteins, we hypothesized that there could be epithelial\u2010specific adaptors involved in polarized sorting. Here, we report the identification of a novel member of the adaptor medium chain family, named \u03bc1B, which is closely related to the previously described \u03bc1A (79% amino acid sequence identity). Northern blotting and in situ hybridization analyses reveal the specific expression of \u03bc1B mRNA in a subset of polarized epithelial and exocrine cells. Yeast two\u2010hybrid analyses show that \u03bc1B is capable of interacting with generic tyrosine\u2010based sorting signals. These observations suggest that \u03bc1B may be involved in protein sorting events specific to polarized cells.</jats:p>",
  "DOI": "10.1016/s0014-5793(99)00432-9",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        25
      ]
    ],
    "date-time": "2002-07-25T12:18:27Z",
    "timestamp": 1027599507000
  },
  "page": "215-220",
  "source": "Crossref",
  "is-referenced-by-count": 221,
  "title": "\u03bc1B, a novel adaptor medium chain expressed in polarized epithelial cells<sup>1</sup>",
  "prefix": "10.1002",
  "volume": "449",
  "author": [
    {
      "given": "Hiroshi",
      "family": "Ohno",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Takuya",
      "family": "Tomemori",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Fubito",
      "family": "Nakatsu",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Yasushi",
      "family": "Okazaki",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Ruben C.",
      "family": "Aguilar",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Heike",
      "family": "Foelsch",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Ira",
      "family": "Mellman",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Takashi",
      "family": "Saito",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Takuji",
      "family": "Shirasawa",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Juan S.",
      "family": "Bonifacino",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        1999,
        4,
        23
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_5_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.cellbio.12.1.575"
    },
    {
      "key": "e_1_2_5_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.110.24.3001"
    },
    {
      "key": "e_1_2_5_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(97)80029-4"
    },
    {
      "key": "e_1_2_5_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/42408"
    },
    {
      "key": "e_1_2_5_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/378096a0"
    },
    {
      "key": "e_1_2_5_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.7.1919"
    },
    {
      "key": "e_1_2_5_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1993.tb05866.x"
    },
    {
      "key": "e_1_2_5_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.42.26300"
    },
    {
      "key": "e_1_2_5_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.126.4.991"
    },
    {
      "key": "e_1_2_5_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.109.8.2133"
    },
    {
      "key": "e_1_2_5_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.137.6.1255"
    },
    {
      "key": "e_1_2_5_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.110.5.597"
    },
    {
      "key": "e_1_2_5_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0962-8924(96)10057-X"
    },
    {
      "key": "e_1_2_5_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(97)80024-5"
    },
    {
      "key": "e_1_2_5_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0962-8924(96)10048-9"
    },
    {
      "key": "e_1_2_5_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(98)80065-3"
    },
    {
      "key": "e_1_2_5_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0167-4889(98)00056-1"
    },
    {
      "key": "e_1_2_5_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7569928"
    },
    {
      "key": "e_1_2_5_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.16.9790"
    },
    {
      "key": "e_1_2_5_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.273.40.25915"
    },
    {
      "key": "e_1_2_5_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00965.x"
    },
    {
      "key": "e_1_2_5_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.5.917"
    },
    {
      "key": "e_1_2_5_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.273.11.6005"
    },
    {
      "key": "e_1_2_5_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.273.15.8638"
    },
    {
      "key": "e_1_2_5_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.8.2148"
    },
    {
      "key": "e_1_2_5_27_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Lennon G. Auffray C. Polymeropoulous M. and Soares M.B. (1996) 33 151\u2013152.",
      "DOI": "10.1006/geno.1996.0177"
    },
    {
      "key": "e_1_2_5_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1991.tb16409.x"
    },
    {
      "key": "e_1_2_5_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(87)90021-2"
    },
    {
      "key": "e_1_2_5_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/aja.1001980102"
    },
    {
      "key": "e_1_2_5_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-5793(96)01500-1"
    },
    {
      "key": "e_1_2_5_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.135.1.53"
    },
    {
      "key": "e_1_2_5_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)36850-4"
    },
    {
      "key": "e_1_2_5_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)41722-4"
    },
    {
      "key": "e_1_2_5_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.128.3.321"
    },
    {
      "key": "e_1_2_5_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.5.10.1093"
    },
    {
      "key": "e_1_2_5_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.4.695"
    },
    {
      "key": "e_1_2_5_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.282.5392.1327"
    },
    {
      "key": "e_1_2_5_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.273.41.26862"
    },
    {
      "key": "e_1_2_5_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.141.3.611"
    },
    {
      "key": "e_1_2_5_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.102.1.48"
    },
    {
      "key": "e_1_2_5_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.274.4.2201"
    },
    {
      "key": "e_1_2_5_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0955-0674(94)90075-2"
    }
  ],
  "container-title": "FEBS Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2899%2900432-9",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(99)00432-9",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2899%2900432-9",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        16
      ]
    ],
    "date-time": "2023-09-16T17:14:57Z",
    "timestamp": 1694884497000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1016/S0014-5793%2899%2900432-9"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1999,
        4,
        23
      ]
    ]
  },
  "references-count": 42,
  "journal-issue": {
    "issue": "2-3",
    "published-print": {
      "date-parts": [
        [
          1999,
          4,
          23
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1016/S0014-5793(99)00432-9"
  ],
  "URL": "http://dx.doi.org/10.1016/S0014-5793(99)00432-9",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        1999,
        4,
        23
      ]
    ]
  }
}