DOI: 10.1016/s0014-5793(99)00254-9. MDM2 interacts with MDMX through their RING finger domains

MDM2 interacts with MDMX through their RING finger domains

10.1016/s0014-5793(99)00254-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The N‐terminus of MDM2 proto‐oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero‐oligomerized with MDMX through their C‐terminal RING finger domains. Yeast two‐hybrid analysis revealed that the hetero‐oligomerization between MDMX and MDM2 was more stable than the homo‐oligomerization of each protein. MDM2 has been shown to be degraded by the ubiquitin‐proteasome pathway, while MDMX was a stable protein. Interaction of MDMX with MDM2 through the C‐terminal RING finger domains resulted in inhibiting degradation of MDM2. These data indicate that MDMX functions as a regulator of MDM2.

Bibliography

Tanimura, S., Ohtsuka, S., Mitsui, K., Shirouzu, K., Yoshimura, A., & Ohtsubo, M. (1999). MDM2 interacts with MDMX through their RING finger domains. FEBS Letters, 447(1), 5â9. Portico.

Authors 6

Shyu Tanimura (first)
Satoshi Ohtsuka (additional)
Kaoru Mitsui (additional)
Kazuo Shirouzu (additional)
Akihiko Yoshimura (additional)
Motoaki Ohtsubo (additional)

References 27 Referenced 241

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Dates

Type	When
Created	23 years ago (July 25, 2002, 1:52 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 9:36 p.m.)
Indexed	1 day, 23 hours ago (Aug. 19, 2025, 6:47 a.m.)
Issued	26 years, 5 months ago (March 19, 1999)
Published	26 years, 5 months ago (March 19, 1999)
Published Online	26 years, 4 months ago (March 29, 1999)
Published Print	26 years, 5 months ago (March 19, 1999)

Funders 0

None

BibTeX

@article{Tanimura_1999, title={MDM2 interacts with MDMX through their RING finger domains}, volume={447}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)00254-9}, DOI={10.1016/s0014-5793(99)00254-9}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Tanimura, Shyu and Ohtsuka, Satoshi and Mitsui, Kaoru and Shirouzu, Kazuo and Yoshimura, Akihiko and Ohtsubo, Motoaki}, year={1999}, month=mar, pages={5–9} }

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