DOI: 10.1016/s0014-5793(99)00251-3. Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis

Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis

10.1016/s0014-5793(99)00251-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The release of two mitochondrial proteins, cytochrome c and apoptosis‐inducing factor (AIF), into the soluble cytoplasm of cells undergoing apoptosis is well established. Using spectrophotometric determination of enzyme activity, the accumulation of adenylate kinase (AK) activity in the cytosolic fraction of apoptotic cells has also been observed recently. However, three isozymes, AK1, AK2 and AK3, have been characterized in mammalian cells and shown to be localized in the cytosol, mitochondrial intermembrane space and mitochondrial matrix, respectively, and it is unknown which one of these isozymes accumulates in the cytosol during apoptosis. We now demonstrate that in apoptotic cells only AK2 was translocated into the cytosol concomitantly with cytochrome c. The amount of AK1 in cytosol, as well as the amount of matrix‐associated AK3, remained unchanged during the apoptotic process. Thus, our data suggest that only intermembrane proteins are released from mitochondria during the early phase of the apoptotic process.

Bibliography

KÃ¶hler, C., Gahm, A., Noma, T., Nakazawa, A., Orrenius, S., & Zhivotovsky, B. (1999). Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis. FEBS Letters, 447(1), 10â12. Portico.

Authors 6

Camilla Köhler (first)
Annie Gahm (additional)
Takafumi Noma (additional)
Atsushi Nakazawa (additional)
Sten Orrenius (additional)
Boris Zhivotovsky (additional)

References 13 Referenced 91

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:16 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 9:35 p.m.)
Indexed	1 month ago (July 24, 2025, 6:55 a.m.)
Issued	26 years, 5 months ago (March 19, 1999)
Published	26 years, 5 months ago (March 19, 1999)
Published Online	26 years, 5 months ago (March 29, 1999)
Published Print	26 years, 5 months ago (March 19, 1999)

Funders 0

None

BibTeX

@article{K_hler_1999, title={Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis}, volume={447}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)00251-3}, DOI={10.1016/s0014-5793(99)00251-3}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Köhler, Camilla and Gahm, Annie and Noma, Takafumi and Nakazawa, Atsushi and Orrenius, Sten and Zhivotovsky, Boris}, year={1999}, month=mar, pages={10–12} }

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