Abstract
We have developed a simple and novel method to isolate a highly pure and active photosystem (PS) II complex, directly from thylakoid membranes. This complex is a discrete particle and contains all the proteins of the oxygen evolving complex and a set of chlorophyll a/b binding proteins. The intactness of both the donor side and the acceptor side has resulted in a very high oxygen evolution activity and therefore offers a superior experimental system to that of PSII enriched membrane fragments in which there is heterogeneity in activities and biochemical composition.
References
17
Referenced
55
- Andersson B. and Franzén L. G. (1992) in: New Comprehensive Biochemistry: Molecular Mechanisms in Bioenergetics (Ernster L. Ed.) pp. 121–143 Elsevier Amsterdam.
-
Andersson B. and Barber J. (1994) in: Advances in Molecular and Cell Biology. Molecular Processes of Photosynthesis (Barber J. Ed.) Vol. 10 pp. 1–53 Jai Press Greenwich CT.
(
10.1016/S1569-2558(08)60394-X
) 10.1016/0014-5793(81)80608-4
10.1016/0005-2728(80)90078-X
10.1111/j.1432-1033.1997.0422a.x
10.1073/pnas.92.1.175
- Andersson B. and Åkerlund H.E. (1987) in: The Light Reactions (Barber J. Ed.) pp. 379–420 Elsevier Science Amsterdam.
10.1046/j.1432-1327.1998.2520268.x
10.1111/j.1399-3054.1994.tb02962.x
10.1007/BF00048597
10.1038/227680a0
10.1104/pp.24.1.1
10.1016/S0014-5793(98)00147-1
- Boekema E.J. van Roon H. Calkoen F. Bassi R. and Dekker J.P. (1998) Proceedings of the XI International Congress on Photosynthesis Budapaest (Garab G. Ed.) (in press) Kluwer Academic Dordrecht.
10.1046/j.1432-1327.1998.2550196.x
10.1146/annurev.arplant.48.1.641
10.1016/S0005-2728(98)00079-6
Dates
Type | When |
---|---|
Created | 23 years, 1 month ago (July 25, 2002, 11:03 a.m.) |
Deposited | 1 year, 11 months ago (Sept. 16, 2023, 3:18 a.m.) |
Indexed | 4 weeks, 1 day ago (Aug. 6, 2025, 8:33 a.m.) |
Issued | 26 years, 5 months ago (March 5, 1999) |
Published | 26 years, 5 months ago (March 5, 1999) |
Published Online | 26 years, 5 months ago (March 15, 1999) |
Published Print | 26 years, 5 months ago (March 5, 1999) |
@article{Eshaghi_1999, title={Isolation of a highly active PSII‐LHCII supercomplex from thylakoid membranes by a direct method}, volume={446}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(99)00149-0}, DOI={10.1016/s0014-5793(99)00149-0}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Eshaghi, Said and Andersson, Bertil and Barber, James}, year={1999}, month=mar, pages={23–26} }