DOI: 10.1016/s0014-5793(98)01449-5. Differential inhibition of transcription from σ<sup>70</sup>‐ and σ<sup>32</sup>‐dependent promoters by rifampicin

Differential inhibition of transcription from σ70‐ and σ32‐dependent promoters by rifampicin

10.1016/s0014-5793(98)01449-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Rifampicin is an antibiotic which binds to the β subunit of prokaryotic RNA polymerases and prevents initiation of transcription. It was found previously that production of heat shock proteins in Escherichia coli cells after a shift from 30°C to 43°C is not completely inhibited by this antibiotic. Here we demonstrate that while activity of a p L‐lacZ fusion (p L is a σ70‐dependent promoter) in E. coli cells is strongly inhibited by rifampicin, a p groE‐lacZ fusion, whose activity is dependent on the σ32 factor, retains significant residual activity even at relatively high rifampicin concentrations. Differential sensitivity to this antibiotic of RNA polymerase holoenzymes containing either the σ70 or the σ32 subunit was confirmed in vitro. Since the effects of an antibiotic that binds to the β subunit can be modulated by the presence of either the σ70 or the σ32 subunit in the holoenzyme, it is tempting to speculate that binding of various σ factors to the core of RNA polymerase results in different conformations of particular holoenzymes, including changes in the core enzyme.

Bibliography

WÄgrzyn, A., Szalewska-PaÅasz, A., BÅaszczak, A., Liberek, K., & WÄgrzyn, G. (1998). Differential inhibition of transcription from Ï70â and Ï32âdependent promoters by rifampicin. FEBS Letters, 440(1â2), 172â174. Portico.

Authors 5

Alicja Węgrzyn (first)
Agnieszka Szalewska-Pałasz (additional)
Adam Błaszczak (additional)
Krzysztof Liberek (additional)
Grzegorz Węgrzyn (additional)

References 16 Referenced 35

Record M.T. Jr. Reznikoff W.S. Craig M.L. McQuade K.L. and Schlax P. (1996) in:Escherichia coliandSalmonella: Cellular and Molecular Biology (Neidhardt F.C. Curtiss III R. Ingraham J.L. Lin E.C.C. Low K.B. Magasanik B. Reznikoff W.S. Riley M. Schaechter M. and Umbarger H.E. Eds.) pp. 792–820 American Society for Microbiology Washington DC.
Lonetto M.A. and Gross C.A. (1996) in:Escherichia coliandSalmonella: Cellular and Molecular Biology (Neidhardt F.C. Curtiss III R. Ingraham J.L. Lin E.C.C. Low K.B. Magasanik B. Reznikoff W.S. Riley M. Schaechter M. and Umbarger H.E. Eds.) p. 821 American Society for Microbiology Washington DC.
10.1007/978-3-642-60691-5_5
10.1016/0022-2836(88)90517-7
10.1006/jmbi.1996.0244
Richardson J.P. and Greenblatt J. (1996) in:Escherichia coliandSalmonella: Cellular and Molecular Biology (Neidhardt F.C. Curtiss III R. Ingraham J.L. Lin E.C.C. Low K.B. Magasanik B. Reznikoff W.S. Riley M. Schaechter M. and Umbarger H.E. Eds.) pp. 822–848 American Society for Microbiology Washington DC.
10.1016/0005-2787(68)90286-4
10.1128/JB.180.9.2475-2483.1998
10.1111/j.1365-2958.1991.tb01980.x
10.1016/0378-1119(94)00829-H
Miller J.H. (1972) Experiments in Molecular Genetics Cold Spring Harbor Laboratory Press Cold Spring Harbor NY.
{'key': 'e_1_2_6_13_1', 'first-page': '181', 'volume': '38', 'author': 'Węgrzyn G', 'year': '1991', 'journal-title': 'Acta Biochim. Pol.'} / Acta Biochim. Pol. by Węgrzyn G (1991)
10.1002/j.1460-2075.1995.tb00190.x
10.1073/pnas.95.8.4241
10.1073/pnas.89.8.3516
{'key': 'e_1_2_6_17_1', 'first-page': '435', 'volume': '201', 'author': 'Fayet O', 'year': '1986', 'journal-title': 'Mol. Gen. Genet.'} / Mol. Gen. Genet. by Fayet O (1986)

Dates

Type	When
Created	22 years, 3 months ago (May 12, 2003, 9:07 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 4:24 a.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 10:07 p.m.)
Issued	26 years, 9 months ago (Nov. 27, 1998)
Published	26 years, 9 months ago (Nov. 27, 1998)
Published Online	26 years, 8 months ago (Dec. 9, 1998)
Published Print	26 years, 9 months ago (Nov. 27, 1998)

Funders 0

None

BibTeX

@article{W_grzyn_1998, title={Differential inhibition of transcription from σ70‐ and σ32‐dependent promoters by rifampicin}, volume={440}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(98)01449-5}, DOI={10.1016/s0014-5793(98)01449-5}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Węgrzyn, Alicja and Szalewska-Pałasz, Agnieszka and Błaszczak, Adam and Liberek, Krzysztof and Węgrzyn, Grzegorz}, year={1998}, month=nov, pages={172–174} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        11
      ]
    ],
    "date-time": "2025-06-11T02:07:39Z",
    "timestamp": 1749607659586
  },
  "reference-count": 16,
  "publisher": "Wiley",
  "issue": "1-2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1998,
            12,
            9
          ]
        ],
        "date-time": "1998-12-09T00:00:00Z",
        "timestamp": 913161600000
      },
      "content-version": "vor",
      "delay-in-days": 12,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1998,
        11,
        27
      ]
    ]
  },
  "abstract": "<jats:p>Rifampicin is an antibiotic which binds to the \u03b2 subunit of prokaryotic RNA polymerases and prevents initiation of transcription. It was found previously that production of heat shock proteins in <jats:italic>Escherichia coli</jats:italic> cells after a shift from 30\u00b0C to 43\u00b0C is not completely inhibited by this antibiotic. Here we demonstrate that while activity of a <jats:italic>p</jats:italic>\n<jats:sub>L</jats:sub>\u2010<jats:italic>lacZ</jats:italic> fusion (<jats:italic>p</jats:italic>\n<jats:sub>L</jats:sub> is a \u03c3<jats:sup>70</jats:sup>\u2010dependent promoter) in <jats:italic>E. coli</jats:italic> cells is strongly inhibited by rifampicin, a <jats:italic>p</jats:italic>\n<jats:sub>groE</jats:sub>\u2010<jats:italic>lacZ</jats:italic> fusion, whose activity is dependent on the \u03c3<jats:sup>32</jats:sup> factor, retains significant residual activity even at relatively high rifampicin concentrations. Differential sensitivity to this antibiotic of RNA polymerase holoenzymes containing either the \u03c3<jats:sup>70</jats:sup> or the \u03c3<jats:sup>32</jats:sup> subunit was confirmed in vitro. Since the effects of an antibiotic that binds to the \u03b2 subunit can be modulated by the presence of either the \u03c3<jats:sup>70</jats:sup> or the \u03c3<jats:sup>32</jats:sup> subunit in the holoenzyme, it is tempting to speculate that binding of various \u03c3 factors to the core of RNA polymerase results in different conformations of particular holoenzymes, including changes in the core enzyme.</jats:p>",
  "DOI": "10.1016/s0014-5793(98)01449-5",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        5,
        13
      ]
    ],
    "date-time": "2003-05-13T01:07:27Z",
    "timestamp": 1052788047000
  },
  "page": "172-174",
  "source": "Crossref",
  "is-referenced-by-count": 35,
  "title": "Differential inhibition of transcription from \u03c3<sup>70</sup>\u2010 and \u03c3<sup>32</sup>\u2010dependent promoters by rifampicin",
  "prefix": "10.1002",
  "volume": "440",
  "author": [
    {
      "given": "Alicja",
      "family": "W\u0119grzyn",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Agnieszka",
      "family": "Szalewska-Pa\u0142asz",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Adam",
      "family": "B\u0142aszczak",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Krzysztof",
      "family": "Liberek",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Grzegorz",
      "family": "W\u0119grzyn",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        1998,
        12,
        9
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "unstructured": "Record M.T. Jr. Reznikoff W.S. Craig M.L. McQuade K.L. and Schlax P. (1996) in:Escherichia coliandSalmonella: Cellular and Molecular Biology (Neidhardt F.C. Curtiss III R. Ingraham J.L. Lin E.C.C. Low K.B. Magasanik B. Reznikoff W.S. Riley M. Schaechter M. and Umbarger H.E. Eds.) pp. 792\u2013820 American Society for Microbiology Washington DC."
    },
    {
      "key": "e_1_2_6_3_1",
      "unstructured": "Lonetto M.A. and Gross C.A. (1996) in:Escherichia coliandSalmonella: Cellular and Molecular Biology (Neidhardt F.C. Curtiss III R. Ingraham J.L. Lin E.C.C. Low K.B. Magasanik B. Reznikoff W.S. Riley M. Schaechter M. and Umbarger H.E. Eds.) p. 821 American Society for Microbiology Washington DC."
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-3-642-60691-5_5"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(88)90517-7"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1996.0244"
    },
    {
      "key": "e_1_2_6_7_1",
      "unstructured": "Richardson J.P. and Greenblatt J. (1996) in:Escherichia coliandSalmonella: Cellular and Molecular Biology (Neidhardt F.C. Curtiss III R. Ingraham J.L. Lin E.C.C. Low K.B. Magasanik B. Reznikoff W.S. Riley M. Schaechter M. and Umbarger H.E. Eds.) pp. 822\u2013848 American Society for Microbiology Washington DC."
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2787(68)90286-4"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.180.9.2475-2483.1998"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.1991.tb01980.x"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(94)00829-H"
    },
    {
      "key": "e_1_2_6_12_1",
      "unstructured": "Miller J.H. (1972) Experiments in Molecular Genetics Cold Spring Harbor Laboratory Press Cold Spring Harbor NY."
    },
    {
      "key": "e_1_2_6_13_1",
      "first-page": "181",
      "volume": "38",
      "author": "W\u0119grzyn G",
      "year": "1991",
      "journal-title": "Acta Biochim. Pol."
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1995.tb00190.x"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.95.8.4241"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.8.3516"
    },
    {
      "key": "e_1_2_6_17_1",
      "first-page": "435",
      "volume": "201",
      "author": "Fayet O",
      "year": "1986",
      "journal-title": "Mol. Gen. Genet."
    }
  ],
  "container-title": "FEBS Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2898%2901449-5",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(98)01449-5",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2898%2901449-5",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        16
      ]
    ],
    "date-time": "2023-09-16T08:24:16Z",
    "timestamp": 1694852656000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1016/S0014-5793%2898%2901449-5"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1998,
        11,
        27
      ]
    ]
  },
  "references-count": 16,
  "journal-issue": {
    "issue": "1-2",
    "published-print": {
      "date-parts": [
        [
          1998,
          11,
          27
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1016/S0014-5793(98)01449-5"
  ],
  "URL": "http://dx.doi.org/10.1016/S0014-5793(98)01449-5",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        1998,
        11,
        27
      ]
    ]
  }
}