DOI: 10.1016/s0014-5793(98)01337-4. <i>Bacillus subtilis</i> DnaG primase stabilises the bacteriophage SPP1 G<i>40</i>P helicase‐ssDNA complex

Bacillus subtilis DnaG primase stabilises the bacteriophage SPP1 G40P helicase‐ssDNA complex

10.1016/s0014-5793(98)01337-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Purified Bacillus subtilis DnaG primase (predicted molecular mass 68.8 kDa) behaves as a monomer in solution. We demonstrate that DnaG physically interacts with bacteriophage SPP1 hexameric helicase G40P (G40P6) in the absence of ATP. G40P6‐ATP forms an unstable complex with ssDNA, and by itself carries out ATP‐driven translocation along a ssDNA template with low processivity. The presence of DnaG in the reaction mixture increased the helicase activity of G40P6 about 3‐fold, but not the ATPase activity. The results presented here suggest that the DnaG protein stabilises the G40P6‐ssDNA complexes.

Bibliography

Ayora, S., Langer, U., & Alonso, J. C. (1998). Bacillus subtilis DnaG primase stabilises the bacteriophage SPP1 G40P helicaseâssDNA complex. FEBS Letters, 439(1â2), 59â62. Portico.

Authors 3

Silvia Ayora (first)
Uwe Langer (additional)
Juan C Alonso (additional)

References 22 Referenced 18

Kornberg A. and Baker T.A. (1992) DNA Replication 2nd edn. W.H. Freeman New York.
10.1016/S0092-8674(00)81039-9
10.1016/S0092-8674(00)80163-4
10.1016/S0021-9258(17)41829-1
10.1074/jbc.271.35.21391
10.1016/S0021-9258(19)50630-5
10.1007/BF00267619
10.1016/0022-2836(94)90061-2
10.1006/jmbi.1997.1060
Barcena M. San Martin C. Weise F. Ayora S. Alonso J.C. and Carazo J.M. (1998) J. Mol. Biol. (in press).
Weise F. Ayora S. and Alonso J.C. (1998) (submitted).
Sambrook J. Fritsch E.F. and Maniatis T. (1989) Molecular Cloning: A Laboratory Handbook Vol. 1–3 Cold Spring Harbor Laboratory Cold Spring Harbor NY.
10.1016/0022-2836(91)90855-Z
Langer U. (1991) Diplomarbeit Freie Universität Berlin.
10.1016/0378-1119(85)90120-9
10.1016/S0021-9258(19)83630-X
10.1073/pnas.93.23.12902
10.1074/jbc.270.13.7462
10.1016/0076-6879(95)62031-1
10.1146/annurev.bi.65.070196.001125
10.1146/annurev.bi.59.070190.001445
10.1016/S0021-9258(17)38564-2

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:53 p.m.)
Deposited	1 year, 11 months ago (Sept. 17, 2023, 1:36 a.m.)
Indexed	1 year, 2 months ago (June 14, 2024, 12:36 p.m.)
Issued	26 years, 9 months ago (Nov. 13, 1998)
Published	26 years, 9 months ago (Nov. 13, 1998)
Published Online	26 years, 9 months ago (Dec. 3, 1998)
Published Print	26 years, 9 months ago (Nov. 13, 1998)

Funders 0

None

BibTeX

@article{Ayora_1998, title={Bacillus subtilis DnaG primase stabilises the bacteriophage SPP1 G40P helicase‐ssDNA complex}, volume={439}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(98)01337-4}, DOI={10.1016/s0014-5793(98)01337-4}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Ayora, Silvia and Langer, Uwe and Alonso, Juan C}, year={1998}, month=nov, pages={59–62} }

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