DOI: 10.1016/s0014-5793(98)00639-5. The compactness of ribonuclease A and reduced ribonuclease A

The compactness of ribonuclease A and reduced ribonuclease A

10.1016/s0014-5793(98)00639-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The compactness of ribonuclease A with intact disulfide bonds and reduced ribonuclease A was investigated by synchrotron small‐angle X‐ray scattering. The R g values and the Kratky plots showed that non‐reduced ribonuclease A maintain a compact shape with a R g value of about 17.3 Å in 8 M urea. The reduced ribonuclease A is more expanded, its R g value is about 20 Å in 50 mM Tris‐HCl buffer at pH 8.1 containing 20 mM DTT. Further expansions of reduced ribonuclease A were observed in the presence of high concentrations of denaturants, indicating that reduced ribonuclease A is more expanded and is in neither a random coil [A. Noppert et al., FEBS Lett. 380 (1996) 179–182] nor a compact denatured state [T.R. Sosnick and J. Trewhella, Biochemistry 31 (1992) 8329–8335]. The four disulfide bonds keep ribonuclease A in a compact state in the presence of high concentrations of urea.

Bibliography

Zhou, J.-M., Fan, Y.-X., Kihara, H., Kimura, K., & Amemiya, Y. (1998). The compactness of ribonuclease A and reduced ribonuclease A. FEBS Letters, 430(3), 275â277. Portico.

Authors 5

Jun-Mei Zhou (first)
Ying-Xin Fan (additional)
Hiroshi Kihara (additional)
Kazumoto Kimura (additional)
Yoshiyuki Amemiya (additional)

References 24 Referenced 14

10.1021/ja00995a002
10.1021/bi00635a011
10.1021/bi00806a045
10.1016/S0022-2836(64)80118-2
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10.1021/bi00536a006
10.1073/pnas.73.6.1853
10.1146/annurev.bi.51.070182.002331
10.1021/bi00652a017
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{'key': 'e_1_2_6_12_1', 'first-page': '51', 'volume': '79', 'author': 'Steiner R.F.', 'year': '1964', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta by Steiner R.F. (1964)
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{'key': 'e_1_2_6_16_1', 'first-page': '1214', 'volume': '35', 'author': 'Hu C.H.', 'year': '1992', 'journal-title': 'Sci. China Ser. B'} / Sci. China Ser. B by Hu C.H. (1992)
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Glatter O. and Kratky O. (1982) in: Small-Angle X-ray Scattering (Glatter O. and Kratky O. Eds.) p. 19 Academic Press New York.
10.1016/0167-4838(87)90192-0
{'key': 'e_1_2_6_23_1', 'first-page': '249', 'volume': '38', 'author': 'Chen L.X.', 'year': '1993', 'journal-title': 'Chin. Sci. Bull.'} / Chin. Sci. Bull. by Chen L.X. (1993)
10.1021/bi00462a019
10.1021/bi00186a015

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 8:19 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 2:50 a.m.)
Indexed	1 year, 11 months ago (Sept. 17, 2023, 1:10 a.m.)
Issued	27 years, 2 months ago (July 3, 1998)
Published	27 years, 2 months ago (July 3, 1998)
Published Online	27 years, 1 month ago (July 29, 1998)
Published Print	27 years, 2 months ago (July 3, 1998)

Funders 0

None

BibTeX

@article{Zhou_1998, title={The compactness of ribonuclease A and reduced ribonuclease A}, volume={430}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(98)00639-5}, DOI={10.1016/s0014-5793(98)00639-5}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Zhou, Jun-Mei and Fan, Ying-Xin and Kihara, Hiroshi and Kimura, Kazumoto and Amemiya, Yoshiyuki}, year={1998}, month=jul, pages={275–277} }

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