DOI: 10.1016/s0014-5793(98)00431-1. The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down‐regulated by doxorubicin

The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down‐regulated by doxorubicin

10.1016/s0014-5793(98)00431-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

We previously isolated the human RPB11 cDNA, encoding the 13.3 kDa subunit of RNA polymerase II, and demonstrated that expression of this subunit is modulated by doxorubicin. Using hRPB11 as bait in a yeast two‐hybrid system, two cDNA variants encoding a second RNA polymerase II subunit, hRPB3, have now been isolated and characterized. These two hRPB3 mRNA species differed in 3′ UTR region length, the longer transcript containing the AU‐rich sequence motif that mediates mRNA degradation. Both hRPB11 and hRPB3 transcripts share a similar pattern of distribution in human adult tissues, with particularly high levels in both heart and skeletal muscle, and the expression of both is down‐regulated by doxorubicin as found previously for the hRPB11 subunit. Taken together, these findings suggest that the interaction between hRPB3 and hRPB11 is fundamental for their function and that this heterodimer is involved in doxorubicin toxicity.

Bibliography

Fanciulli, M., Bruno, T., Di Padova, M., De Angelis, R., Lovari, S., Floridi, A., & Passananti, C. (1998). The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and downâregulated by doxorubicin. FEBS Letters, 427(2), 236â240. Portico.

Authors 7

Maurizio Fanciulli (first)
Tiziana Bruno (additional)
Monica Di Padova (additional)
Roberta De Angelis (additional)
Sarah Lovari (additional)
Aristide Floridi (additional)
Claudio Passananti (additional)

References 35 Referenced 16

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Dates

Type	When
Created	23 years ago (July 25, 2002, 3:16 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 4:55 a.m.)
Indexed	11 months, 1 week ago (Sept. 16, 2024, 4 p.m.)
Issued	27 years, 3 months ago (May 8, 1998)
Published	27 years, 3 months ago (May 8, 1998)
Published Online	26 years, 8 months ago (Dec. 18, 1998)
Published Print	27 years, 3 months ago (May 8, 1998)

Funders 0

None

BibTeX

@article{Fanciulli_1998, title={The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down‐regulated by doxorubicin}, volume={427}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(98)00431-1}, DOI={10.1016/s0014-5793(98)00431-1}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Fanciulli, Maurizio and Bruno, Tiziana and Di Padova, Monica and De Angelis, Roberta and Lovari, Sarah and Floridi, Aristide and Passananti, Claudio}, year={1998}, month=may, pages={236–240} }

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