DOI: 10.1016/s0014-5793(98)00337-8. Stability and functionality of cysteine‐less FOF1 ATP synthase from Escherichia coli

Stability and functionality of cysteine‐less FOF1 ATP synthase from Escherichia coli

10.1016/s0014-5793(98)00337-8

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

All 21 native cysteines in the Escherichia coli FOF1 ATP synthase were replaced by alanines. In isolated E. coli membranes, ATP‐dependent proton pumping, turnover of ATP hydrolysis and steady‐state transition state thermodynamic parameters of the cysteine‐less enzyme were similar to wild‐type. The cysteine‐less enzyme was solubilized in n‐octyl β‐d‐glucopyranoside, purified by affinity chromatography, and reconstituted into pre‐formed liposomes made from E. coli lipids. The properties of the reconstituted, purified enzyme were not significantly different from the membranous enzyme. These data demonstrate that cysteine‐less FOF1 is biochemically stable and has functionality similar to wild‐type.

Bibliography

Kuo, P. H., Ketchum, C. J., & Nakamoto, R. K. (1998). Stability and functionality of cysteineâless FOF1 ATP synthase from Escherichia coli. FEBS Letters, 426(2), 217â220. Portico.

Authors 3

Phillip H Kuo (first)
Christian J Ketchum (additional)
Robert K Nakamoto (additional)

References 20 Referenced 42

10.1007/s002329900061
10.1146/annurev.biochem.66.1.717
10.1016/S0005-2728(96)00121-1
10.1016/S0968-0004(97)01129-8
10.1128/jb.160.3.1055-1060.1984
10.1128/JB.134.3.1141-1156.1978
10.1042/bj3300707
Sambrook J. Fritsch E.F. and Maniatis T. (1989) Molecular Cloning: A Laboratory Manual 2nd Ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor NY.
Mullis K.B. Ferré F. and Gibbs R.A. (1994) in: K.B. Mullis F. Ferré and R.A. Gibbs (Eds.) The Polymerase Chain Reaction Birkhäuser Boston. (10.1007/978-1-4612-0257-8)
10.1038/nbt1088-1204
10.1016/0005-2728(96)00056-4
10.1073/pnas.71.7.2725
10.1016/S0021-9258(19)52451-6
10.1016/0003-2697(85)90445-2
10.1074/jbc.272.4.2300
10.1074/jbc.270.23.14042
{'key': 'e_1_2_6_18_1', 'first-page': '463', 'volume': '75', 'author': 'Fabiato A', 'year': '1979', 'journal-title': 'J. Physiol. (Paris)'} / J. Physiol. (Paris) by Fabiato A (1979)
10.1016/S0021-9258(18)54545-2
{'key': 'e_1_2_6_20_1', 'first-page': '112961', 'volume': '36', 'author': 'Al-Shawi M.K', 'year': '1997', 'journal-title': 'Biochemistry'} / Biochemistry by Al-Shawi M.K (1997)
Hubbell W.L. and Altenbach C. (1994) in: S.H. White (Ed.) Membrane Protein Structure: Experimental Approaches Oxford University Press New York pp. 224–248. (10.1007/978-1-4614-7515-6_10)

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:53 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 10:30 p.m.)
Indexed	2 months ago (June 27, 2025, 6:35 a.m.)
Issued	27 years, 4 months ago (April 17, 1998)
Published	27 years, 4 months ago (April 17, 1998)
Published Online	27 years, 2 months ago (June 15, 1998)
Published Print	27 years, 4 months ago (April 17, 1998)

Funders 0

None

BibTeX

@article{Kuo_1998, title={Stability and functionality of cysteine‐less FOF1 ATP synthase from Escherichia coli}, volume={426}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(98)00337-8}, DOI={10.1016/s0014-5793(98)00337-8}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Kuo, Phillip H and Ketchum, Christian J and Nakamoto, Robert K}, year={1998}, month=apr, pages={217–220} }

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