DOI: 10.1016/s0014-5793(98)00333-0. Topological localization of cysteine 74 in the GABA transporter, GAT1, and its importance in ion binding and permeation

Topological localization of cysteine 74 in the GABA transporter, GAT1, and its importance in ion binding and permeation

10.1016/s0014-5793(98)00333-0

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Xenopus oocytes expressing the GABA transporter GAT1 were exposed to membrane‐impermeant sulfhydryl reagents, resulting in decreased GABA transport current, decreased capacitive charge movements, and increased Na+ and Li+ leakage currents. Mutation of cysteine 74 to alanine (C74A) eliminated these effects. The W68S and W68L mutations significantly increased and decreased the transporter's sensitivity, respectively, to sulfhydryl reagents. At each of the positions 73 through 76, cysteine residues were accessible to external MTSET. These findings, together with recent evidence placing the HD2–HD3 loop on the extracellular side, suggest that the HD2 region does not traverse the membrane.

Bibliography

Yu, N., Cao, Y., Mager, S., & Lester, H. A. (1998). Topological localization of cysteine 74 in the GABA transporter, GAT1, and its importance in ion binding and permeation. FEBS Letters, 426(2), 174â178. Portico.

Authors 4

Nam Yu (first)
Yongwei Cao (additional)
Sela Mager (additional)
Henry A. Lester (additional)

References 29 Referenced 32

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:16 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 10:30 p.m.)
Indexed	3 months ago (June 6, 2025, 5:08 a.m.)
Issued	27 years, 4 months ago (April 17, 1998)
Published	27 years, 4 months ago (April 17, 1998)
Published Online	27 years, 2 months ago (June 15, 1998)
Published Print	27 years, 4 months ago (April 17, 1998)

Funders 0

None

BibTeX

@article{Yu_1998, title={Topological localization of cysteine 74 in the GABA transporter, GAT1, and its importance in ion binding and permeation}, volume={426}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(98)00333-0}, DOI={10.1016/s0014-5793(98)00333-0}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Yu, Nam and Cao, Yongwei and Mager, Sela and Lester, Henry A.}, year={1998}, month=apr, pages={174–178} }

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