DOI: 10.1016/s0014-5793(98)00147-1. Specific association of photosystem II and light‐harvesting complex II in partially solubilized photosystem II membranes

Specific association of photosystem II and light‐harvesting complex II in partially solubilized photosystem II membranes

10.1016/s0014-5793(98)00147-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

In this study, we report the structural characterization of photosystem II complexes obtained from partially solubilized photosystem II membranes. Direct observation by electron microscopy, within a few minutes after a mild disruption of the membranes with the detergent n‐dodecyl‐α,d‐maltoside, revealed the presence of several large supramolecular complexes. Images of these complexes were subjected to multivariate statistical analysis and classification procedures, resolving a new complex consisting of the previously characterized dimeric supercomplex of photosystem II and light‐harvesting complex II [Boekema et al., Proc. Natl. Acad. Sci. USA 92 (1995) 175–179] and two additional, symmetrically organized protein masses each containing a second type of trimeric light‐harvesting II complex. We conclude that large and labile integral membrane proteins, such as photosystem II, can be quickly structurally characterized without extensive purification.

Bibliography

Boekema, E. J., van Roon, H., & Dekker, J. P. (1998). Specific association of photosystem II and lightâharvesting complex II in partially solubilized photosystem II membranes. FEBS Letters, 424(1â2), 95â99. Portico.

Authors 3

Egbert J Boekema (first)
Henny van Roon (additional)
Jan P Dekker (additional)

References 16 Referenced 61

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:16 p.m.)
Deposited	1 year, 11 months ago (Sept. 17, 2023, 3:09 a.m.)
Indexed	1 month ago (Aug. 6, 2025, 9:27 a.m.)
Issued	27 years, 6 months ago (March 6, 1998)
Published	27 years, 6 months ago (March 6, 1998)
Published Online	27 years, 5 months ago (March 17, 1998)
Published Print	27 years, 6 months ago (March 6, 1998)

Funders 0

None

BibTeX

@article{Boekema_1998, title={Specific association of photosystem II and light‐harvesting complex II in partially solubilized photosystem II membranes}, volume={424}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(98)00147-1}, DOI={10.1016/s0014-5793(98)00147-1}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Boekema, Egbert J and van Roon, Henny and Dekker, Jan P}, year={1998}, month=mar, pages={95–99} }

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