DOI: 10.1016/s0014-5793(98)00035-0. Ionic currents underlying HTRP3 mediated agonist‐dependent Ca<sup>2+</sup> influx in stably transfected HEK293 cells

Ionic currents underlying HTRP3 mediated agonist‐dependent Ca2+ influx in stably transfected HEK293 cells

10.1016/s0014-5793(98)00035-0

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

hTrp3 is a human homologue of the Drosophila gene responsible for a transient receptor potential (trp) mutation. When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic receptors, respectively. Agonist evoked currents reversed polarity near 0 mV in physiological ionic conditions and were associated with a significant increase in the current variance. These results suggest the involvement of a non‐selective cation channel with relatively large unitary amplitude. Consistent with this, resolved unitary events had a conductance of approximately 60 pS in the negative voltage range and an extrapolated reversal potential near 0 mV.

Bibliography

Hurst, R. S., Zhu, X., Boulay, G., Birnbaumer, L., & Stefani, E. (1998). Ionic currents underlying HTRP3 mediated agonistâdependent Ca2+ influx in stably transfected HEK293 cells. FEBS Letters, 422(3), 333â338. Portico.

Authors 5

Raymond S Hurst (first)
Xi Zhu (additional)
Guylain Boulay (additional)
Lutz Birnbaumer (additional)
Enrico Stefani (additional)

References 30 Referenced 88

10.1073/pnas.93.2.571
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10.1152/ajpcell.1995.269.5.C1332
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10.1016/S0092-8674(00)80302-5

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:53 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 4:53 a.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 3:56 p.m.)
Issued	27 years, 6 months ago (Feb. 6, 1998)
Published	27 years, 6 months ago (Feb. 6, 1998)
Published Online	27 years, 6 months ago (Feb. 19, 1998)
Published Print	27 years, 6 months ago (Feb. 6, 1998)

Funders 0

None

BibTeX

@article{Hurst_1998, title={Ionic currents underlying HTRP3 mediated agonist‐dependent Ca2+ influx in stably transfected HEK293 cells}, volume={422}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(98)00035-0}, DOI={10.1016/s0014-5793(98)00035-0}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Hurst, Raymond S and Zhu, Xi and Boulay, Guylain and Birnbaumer, Lutz and Stefani, Enrico}, year={1998}, month=feb, pages={333–338} }

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