DOI: 10.1016/s0014-5793(97)01537-8. Self‐oligomerization of NACP, the precursor protein of the non‐amyloid β/A4 protein (Aβ) component of Alzheimer's disease amyloid, observed in the presence of a C‐terminal Aβ fragment (residues 25

Self‐oligomerization of NACP, the precursor protein of the non‐amyloid β/A4 protein (Aβ) component of Alzheimer's disease amyloid, observed in the presence of a C‐terminal Aβ fragment (residues 25–35)

10.1016/s0014-5793(97)01537-8

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

NACP, the precursor protein of the non‐amyloid β/A4 protein (Aβ) component of Alzheimer's disease (AD) amyloid, also known as α‐synuclein, was suggested to seed amyloid plaque formation in AD by stimulating Aβ aggregation. We have demonstrated that NACP experienced self‐oligomerization only in the presence of a modified Aβ fragment (Aβ25–35) by using dicyclohexylcarbodiimide. This NACP oligomerization, appearing as a discrete ladder on a Tricine SDS‐PAGE, was not observed with other Aβ peptides such as the reverse peptide Aβ35–25 and Aβ1–40, indicating this process was specific not only for the C‐terminal peptide sequence of the Aβ but also for its orientation. It might be, therefore, suggested that the NACP self‐oligomers formed only in the presence of a N‐terminally truncated Aβ peptide could act as a nucleation center for plaque formation during AD development.

Bibliography

Paik, S. R., Lee, J.-H., Kim, D.-H., Chang, C.-S., & Kim, Y.-S. (1998). Selfâoligomerization of NACP, the precursor protein of the nonâamyloid Î²/A4 protein (AÎ²) component of Alzheimerâs disease amyloid, observed in the presence of a Câterminal AÎ² fragment (residues 25â35). FEBS Letters, 421(1), 73â76. Portico.

Authors 5

Seung R Paik (first)
Ju-Hyun Lee (additional)
Do-Hyung Kim (additional)
Chung-Soon Chang (additional)
Young-Sik Kim (additional)

References 19 Referenced 59

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:53 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 3:30 a.m.)
Indexed	1 month ago (July 30, 2025, 11:30 a.m.)
Issued	27 years, 7 months ago (Jan. 2, 1998)
Published	27 years, 7 months ago (Jan. 2, 1998)
Published Online	27 years, 6 months ago (Feb. 10, 1998)
Published Print	27 years, 7 months ago (Jan. 2, 1998)

Funders 0

None

BibTeX

@article{Paik_1998, title={Self‐oligomerization of NACP, the precursor protein of the non‐amyloid β/A4 protein (Aβ) component of Alzheimer’s disease amyloid, observed in the presence of a C‐terminal Aβ fragment (residues 25–35)}, volume={421}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)01537-8}, DOI={10.1016/s0014-5793(97)01537-8}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Paik, Seung R and Lee, Ju-Hyun and Kim, Do-Hyung and Chang, Chung-Soon and Kim, Young-Sik}, year={1998}, month=jan, pages={73–76} }

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