DOI: 10.1016/s0014-5793(97)01503-2. Isolation and characterization of the circulating form of human endostatin

Isolation and characterization of the circulating form of human endostatin

10.1016/s0014-5793(97)01503-2

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Recently, fragments of extracellular proteins, including endostatin, were defined as a novel group of angiogenesis inhibitors. In this study, human plasma equivalent hemofiltrate was used as a source for the purification of high molecular weight peptides (10–20 kDa), and the isolation and identification of circulating human endostatin are described. The purification of this C‐terminal fragment of collagen α1(XVIII) was guided by MALDI‐MS and the exact molecular mass determined by ESI‐MS was found to be 18 494 Da. N‐terminal sequencing revealed the identity of this putative angiogenesis inhibitor and its close relation to mouse endostatin. The cysteine residues 1–3 and 2–4 in the molecule are linked by disulfide bridges. In vitro biological characterization of the native protein demonstrated no anti‐proliferative activity on different endothelial cell types. These data indicate that human endostatin, which is a putative angiogenesis inhibitor, is present in the circulation.

Bibliography

StÃ¤ndker, L., Schrader, M., Kanse, S. M., JÃ¼rgens, M., Forssmann, W.-G., & Preissner, K. T. (1997). Isolation and characterization of the circulating form of human endostatin. FEBS Letters, 420(2â3), 129â133. Portico.

Authors 6

Ludger Ständker (first)
Michael Schrader (additional)
Sandip M Kanse (additional)
Michael Jürgens (additional)
Wolf-Georg Forssmann (additional)
Klaus T Preissner (additional)

References 23 Referenced 87

10.1093/jnci/82.1.4
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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:53 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 3:17 a.m.)
Indexed	1 month ago (July 23, 2025, 8:55 a.m.)
Issued	27 years, 7 months ago (Dec. 29, 1997)
Published	27 years, 7 months ago (Dec. 29, 1997)
Published Online	27 years, 7 months ago (Jan. 14, 1998)
Published Print	27 years, 7 months ago (Dec. 29, 1997)

Funders 0

None

BibTeX

@article{St_ndker_1997, title={Isolation and characterization of the circulating form of human endostatin}, volume={420}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)01503-2}, DOI={10.1016/s0014-5793(97)01503-2}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Ständker, Ludger and Schrader, Michael and Kanse, Sandip M and Jürgens, Michael and Forssmann, Wolf-Georg and Preissner, Klaus T}, year={1997}, month=dec, pages={129–133} }

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