DOI: 10.1016/s0014-5793(97)01467-1. RanBP1 is crucial for the release of RanGTP from importin β‐related nuclear transport factors

RanBP1 is crucial for the release of RanGTP from importin β‐related nuclear transport factors

10.1016/s0014-5793(97)01467-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Nucleocytoplasmic transport appears mediated by shuttling transport receptors that bind RanGTP as a means to regulate interactions with their cargoes. The receptor·RanGTP complexes are kinetically very stable with nucleotide exchange and GTP hydrolysis being blocked, predicting that a specific disassembly mechanism exists. Here we show in three cases receptor·RanGTP·RanBP1 complexes to be the key disassembly intermediates, where RanBP1 stimulates the off‐rate at the receptor/RanGTP interface by more than two orders of magnitude. The transiently released RanGTP·RanBP1 complex is then induced by RanGAP to hydrolyse GTP, preventing the receptor to rebind RanGTP. The efficient release of importin β from RanGTP requires importin α, in addition to RanBP1.

Authors 2

F.Ralf Bischoff (first)
Dirk Görlich (additional)

References 55 Referenced 220

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:53 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 1:24 a.m.)
Indexed	1 week, 1 day ago (Aug. 21, 2025, 12:36 p.m.)
Issued	27 years, 8 months ago (Dec. 15, 1997)
Published	27 years, 8 months ago (Dec. 15, 1997)
Published Online	27 years, 7 months ago (Jan. 14, 1998)
Published Print	27 years, 8 months ago (Dec. 15, 1997)

Funders 0

None

BibTeX

@article{Bischoff_1997, title={RanBP1 is crucial for the release of RanGTP from importin β‐related nuclear transport factors}, volume={419}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)01467-1}, DOI={10.1016/s0014-5793(97)01467-1}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Bischoff, F.Ralf and Görlich, Dirk}, year={1997}, month=dec, pages={249–254} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        21
      ]
    ],
    "date-time": "2025-08-21T16:36:46Z",
    "timestamp": 1755794206684
  },
  "reference-count": 55,
  "publisher": "Wiley",
  "issue": "2-3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1998,
            1,
            14
          ]
        ],
        "date-time": "1998-01-14T00:00:00Z",
        "timestamp": 884736000000
      },
      "content-version": "vor",
      "delay-in-days": 30,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1997,
        12,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Nucleocytoplasmic transport appears mediated by shuttling transport receptors that bind RanGTP as a means to regulate interactions with their cargoes. The receptor\u00b7RanGTP complexes are kinetically very stable with nucleotide exchange and GTP hydrolysis being blocked, predicting that a specific disassembly mechanism exists. Here we show in three cases receptor\u00b7RanGTP\u00b7RanBP1 complexes to be the key disassembly intermediates, where RanBP1 stimulates the off\u2010rate at the receptor/RanGTP interface by more than two orders of magnitude. The transiently released RanGTP\u00b7RanBP1 complex is then induced by RanGAP to hydrolyse GTP, preventing the receptor to rebind RanGTP. The efficient release of importin \u03b2 from RanGTP requires importin \u03b1, in addition to RanBP1.</jats:p>",
  "DOI": "10.1016/s0014-5793(97)01467-1",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        25
      ]
    ],
    "date-time": "2002-07-25T17:53:27Z",
    "timestamp": 1027619607000
  },
  "page": "249-254",
  "source": "Crossref",
  "is-referenced-by-count": 220,
  "title": "RanBP1 is crucial for the release of RanGTP from importin \u03b2\u2010related nuclear transport factors",
  "prefix": "10.1002",
  "volume": "419",
  "author": [
    {
      "given": "F.Ralf",
      "family": "Bischoff",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Dirk",
      "family": "G\u00f6rlich",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        1998,
        1,
        14
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(97)80015-4"
    },
    {
      "key": "e_1_2_6_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mmbr.61.2.193-211.1997"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/386779a0"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80361-X"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81315-X"
    },
    {
      "key": "e_1_2_6_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(96)00583-2"
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80372-4"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80173-7"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/excr.1996.0369"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.110.11.1325"
    },
    {
      "key": "e_1_2_6_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80370-0"
    },
    {
      "key": "e_1_2_6_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80371-2"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/365661a0"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.123.6.1649"
    },
    {
      "key": "e_1_2_6_16_1",
      "unstructured": "Izaurralde E. Kutay U. Mattaj I.W. and G\u00f6rlich D. (1997) EMBO J. to appear."
    },
    {
      "key": "e_1_2_6_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.133.3.485"
    },
    {
      "key": "e_1_2_6_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.133.6.1163"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.276.5320.1842"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7534442"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.1.225"
    },
    {
      "key": "e_1_2_6_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/354080a0"
    },
    {
      "key": "e_1_2_6_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.7.2587"
    },
    {
      "key": "e_1_2_6_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.5.1749"
    },
    {
      "key": "e_1_2_6_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.20.11860"
    },
    {
      "key": "e_1_2_6_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.130.5.1017"
    },
    {
      "key": "e_1_2_6_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/366585a0"
    },
    {
      "key": "e_1_2_6_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1995.tb07049.x"
    },
    {
      "key": "e_1_2_6_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.24.14405"
    },
    {
      "key": "e_1_2_6_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.109.4.1389"
    },
    {
      "key": "e_1_2_6_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.111.2.309"
    },
    {
      "key": "e_1_2_6_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.4.6.569"
    },
    {
      "key": "e_1_2_6_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.135.6.1457"
    },
    {
      "key": "e_1_2_6_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81862-0"
    },
    {
      "key": "e_1_2_6_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.134.5.1157"
    },
    {
      "key": "e_1_2_6_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00943.x"
    },
    {
      "key": "e_1_2_6_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.4.807"
    },
    {
      "key": "e_1_2_6_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.138.1.65"
    },
    {
      "key": "e_1_2_6_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.135.3.559"
    },
    {
      "key": "e_1_2_6_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.5.2357"
    },
    {
      "key": "e_1_2_6_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.17.9.5087"
    },
    {
      "key": "e_1_2_6_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.10.5313"
    },
    {
      "key": "e_1_2_6_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.20.6237"
    },
    {
      "key": "e_1_2_6_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90181-7"
    },
    {
      "key": "e_1_2_6_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(95)57019-5"
    },
    {
      "key": "e_1_2_6_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00095a023"
    },
    {
      "key": "e_1_2_6_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)78123-X"
    },
    {
      "key": "e_1_2_6_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi970524k"
    },
    {
      "key": "e_1_2_6_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.1.551"
    },
    {
      "key": "e_1_2_6_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.31.19538"
    },
    {
      "key": "e_1_2_6_51_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.6.1153"
    },
    {
      "key": "e_1_2_6_52_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.8.6.945"
    },
    {
      "key": "e_1_2_6_53_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(78)90108-3"
    },
    {
      "key": "e_1_2_6_54_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1995.tb00221.x"
    },
    {
      "key": "e_1_2_6_55_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/376184a0"
    },
    {
      "key": "e_1_2_6_56_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.23.14209"
    }
  ],
  "container-title": "FEBS Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2897%2901467-1",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(97)01467-1",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2897%2901467-1",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        16
      ]
    ],
    "date-time": "2023-09-16T05:24:31Z",
    "timestamp": 1694841871000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1016/S0014-5793%2897%2901467-1"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1997,
        12,
        15
      ]
    ]
  },
  "references-count": 55,
  "journal-issue": {
    "issue": "2-3",
    "published-print": {
      "date-parts": [
        [
          1997,
          12,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1016/S0014-5793(97)01467-1"
  ],
  "URL": "http://dx.doi.org/10.1016/S0014-5793(97)01467-1",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        1997,
        12,
        15
      ]
    ]
  }
}