DOI: 10.1016/s0014-5793(97)01332-x. <i>Arabidopsis thaliana</i> class IV chitinase is early induced during the interaction with <i>Xanthomonas campestris</i>

Arabidopsis thaliana class IV chitinase is early induced during the interaction with Xanthomonas campestris

10.1016/s0014-5793(97)01332-x

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Endochitinases are widely distributed among higher plants, including a number of important crop species. They are generally considered to be involved in plant defence against potential pathogens. We have cloned a class IV chitinase gene (AtchitIV) from Arabidopsis thaliana. Southern blot analysis allowed the detection of two cross‐hybridising genes in the A. thaliana genome. AtchitIV transcripts are detected in seedpods, but not in roots, inflorescence stems, leaves and flowers of healthy plants. The transcripts accumulated very rapidly in leaves after inoculation with Xanthomonas campestris. Maximum mRNA accumulation was reached one hour after infection and decreased to very low levels 72 hours after induction. This result suggests an involvement of AtchitIV in the initial events of the hypersensitive reaction. Nevertheless, A. thaliana plants transformed with the gus gene under the control of a class IV chitinase bean promoter, showed GUS activity in seed embryos. These data, together with the constitutive expression of the endogenous gene in the seedpods, points to additional physiological roles for this protein.

Bibliography

de A. Gerhardt, L. B., Sachetto-Martins, G., Contarini, M. G., Sandroni, M., de P. Ferreira, R., de Lima, V. M., Cordeiro, M. C., de Oliveira, D. E., & Margis-Pinheiro, M. (1997). Arabidopsis thaliana class IV chitinase is early induced during the interaction with Xanthomonas campestris. FEBS Letters, 419(1), 69â75. Portico.

Authors 9

Liliane B de A. Gerhardt (first)
Gilberto Sachetto-Martins (additional)
Maria G Contarini (additional)
Mariana Sandroni (additional)
Rodrigo de P. Ferreira (additional)
Viviane M de Lima (additional)
Maria C Cordeiro (additional)
Dulce E de Oliveira (additional)
Márcia Margis-Pinheiro (additional)

References 61 Referenced 58

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 12:08 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 8:59 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 5, 2025, 8:48 a.m.)
Issued	27 years, 8 months ago (Dec. 8, 1997)
Published	27 years, 8 months ago (Dec. 8, 1997)
Published Online	27 years, 7 months ago (Jan. 13, 1998)
Published Print	27 years, 8 months ago (Dec. 8, 1997)

Funders 0

None

BibTeX

@article{de_A_Gerhardt_1997, title={Arabidopsis thaliana class IV chitinase is early induced during the interaction with Xanthomonas campestris}, volume={419}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)01332-x}, DOI={10.1016/s0014-5793(97)01332-x}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={de A. Gerhardt, Liliane B and Sachetto-Martins, Gilberto and Contarini, Maria G and Sandroni, Mariana and de P. Ferreira, Rodrigo and de Lima, Viviane M and Cordeiro, Maria C and de Oliveira, Dulce E and Margis-Pinheiro, Márcia}, year={1997}, month=dec, pages={69–75} }

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  "URL": "http://dx.doi.org/10.1016/S0014-5793(97)01332-X",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        1997,
        12,
        8
      ]
    ]
  }
}