DOI: 10.1016/s0014-5793(97)01062-4. Selection and identification of single domain antibody fragments from camel heavy‐chain antibodies

Selection and identification of single domain antibody fragments from camel heavy‐chain antibodies

10.1016/s0014-5793(97)01062-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Functional heavy‐chain γ‐immunoglobulins lacking light chains occur naturally in Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire of the variable domains of its heavy‐chain antibodies and panning, leading to the successful identification of minimum sized antigen binders. The recombinant binders are expressed well in E. coli, extremely stable, highly soluble, and react specifically and with high affinity to the antigens. This approach can be viewed as a general route to obtain small binders with favourable characteristics and valuable perspectives as modular building blocks to manufacture multispecific or multifunctional chimaeric proteins.

Bibliography

Arbabi Ghahroudi, M., Desmyter, A., Wyns, L., Hamers, R., & Muyldermans, S. (1997). Selection and identification of single domain antibody fragments from camel heavyâchain antibodies. FEBS Letters, 414(3), 521â526. Portico.

Authors 5

M Arbabi Ghahroudi (first)
A Desmyter (additional)
L Wyns (additional)
R Hamers (additional)
S Muyldermans (additional)

References 31 Referenced 644

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 8:19 a.m.)
Deposited	1 year, 11 months ago (Sept. 17, 2023, 3:04 a.m.)
Indexed	42 minutes ago (Sept. 2, 2025, 9:23 a.m.)
Issued	27 years, 11 months ago (Sept. 15, 1997)
Published	27 years, 11 months ago (Sept. 15, 1997)
Published Online	27 years, 7 months ago (Jan. 14, 1998)
Published Print	27 years, 11 months ago (Sept. 15, 1997)

Funders 0

None

BibTeX

@article{Arbabi_Ghahroudi_1997, title={Selection and identification of single domain antibody fragments from camel heavy‐chain antibodies}, volume={414}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)01062-4}, DOI={10.1016/s0014-5793(97)01062-4}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Arbabi Ghahroudi, M and Desmyter, A and Wyns, L and Hamers, R and Muyldermans, S}, year={1997}, month=sep, pages={521–526} }

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