DOI: 10.1016/s0014-5793(97)00753-9. Potential CRE suppression by familial Alzheimer's mutants of APP independent of adenylyl cyclase regulation

Potential CRE suppression by familial Alzheimer's mutants of APP independent of adenylyl cyclase regulation

10.1016/s0014-5793(97)00753-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

In familial Alzheimer's disease (FAD), mutations to I, F, and G have been discovered at V642 in the neuron‐specific version of the amyloid precursor protein APP695. It has been found that expression of each FAD mutant suppresses the transcriptional activity of the cAMP response element CRE in a Gαo‐dependent manner in a COS cell clone NK1 [Ikezu et al. (1996) EMBO J. 15, 2468–2475]. Here we show that adenylyl cyclase (AC) inhibition is probably not the prerequisite for this pathway. First, expression of each FAD mutant in NK1 cells had no effect on AC activity stimulated by cholera toxin and by mutationally activated Gαs, although the same expression completely repressed the stimulated CRE. Second, a transfected activating mutant of Gαo inhibited CRE without detectable suppression of AC, whereas similarly transfected activating Gαi2 inhibited both AC and CRE. Third, FAD mutant‐induced inhibition occurred for CRE activity stimulated by dibutyryl cAMP. These data suggest that CRE suppression by FAD mutants of APP could occur independently of AC.

Bibliography

Giambarella, U., Murayama, Y., Ikezu, T., Fujita, T., & Nishimoto, I. (1997). Potential CRE suppression by familial Alzheimerâs mutants of APP independent of adenylyl cyclase regulation. FEBS Letters, 412(1), 97â101. Portico.

Authors 5

Ugo Giambarella (first)
Yoshitake Murayama (additional)
Tsuneya Ikezu (additional)
Toshiro Fujita (additional)
Ikuo Nishimoto (additional)

References 34 Referenced 8

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 8:19 a.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 11:45 p.m.)
Indexed	1 month, 1 week ago (July 25, 2025, 6:53 a.m.)
Issued	28 years, 1 month ago (July 21, 1997)
Published	28 years, 1 month ago (July 21, 1997)
Published Online	27 years, 10 months ago (Nov. 3, 1997)
Published Print	28 years, 1 month ago (July 21, 1997)

Funders 0

None

BibTeX

@article{Giambarella_1997, title={Potential CRE suppression by familial Alzheimer’s mutants of APP independent of adenylyl cyclase regulation}, volume={412}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)00753-9}, DOI={10.1016/s0014-5793(97)00753-9}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Giambarella, Ugo and Murayama, Yoshitake and Ikezu, Tsuneya and Fujita, Toshiro and Nishimoto, Ikuo}, year={1997}, month=jul, pages={97–101} }

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