DOI: 10.1016/s0014-5793(97)00657-1. Molecular cloning of a novel phosphorylation‐dependent inhibitory protein of protein phosphatase‐1 (CPI17) in smooth muscle: its specific localization in smooth muscle<sup>1</sup>

Molecular cloning of a novel phosphorylation‐dependent inhibitory protein of protein phosphatase‐1 (CPI17) in smooth muscle: its specific localization in smooth muscle1

10.1016/s0014-5793(97)00657-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The cDNA encoding a phosphorylation‐dependent inhibitory protein of protein phosphatase‐1 (PP1) was isolated from a porcine aorta library. The coding region represented the complete amino acid sequence of this protein comprised of a novel 147‐residue polypeptide, which we termed CPI17, a 17‐kDa PKC‐potentiated inhibitory protein of PP1. As well as the native CPI17 from porcine aorta, the recombinant protein completely suppressed the PP1 activity (IC50=0.18 nM) by the stoichiometric thiophosphorylation. The CPI17 mRNA is expressed in smooth muscle tissues such as aorta and bladder, whereas little expression was observed in heart, skeletal muscle, and non‐muscle tissues. These results suggest a specific regulatory mechanism of the PP1 activity through CPI17 in smooth muscle.

Bibliography

Eto, M., Senba, S., Morita, F., & Yazawa, M. (1997). Molecular cloning of a novel phosphorylationâdependent inhibitory protein of protein phosphataseâ1 (CPI17) in smooth muscle: its specific localization in smooth muscle1. FEBS Letters, 410(2â3), 356â360. Portico.

Authors 4

Masumi Eto (first)
Shingo Senba (additional)
Fumi Morita (additional)
Michio Yazawa (additional)

References 30 Referenced 212

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J. Sambrook E.F. Fritsch T. Maniatis Molecular Cloning: A Laboratory Manual 2nd EDN. Cold Spring Harbor Labratory Cold Spring Harbor NY 1989.
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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:54 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 2:09 p.m.)
Indexed	1 month ago (Aug. 2, 2025, midnight)
Issued	28 years, 2 months ago (June 30, 1997)
Published	28 years, 2 months ago (June 30, 1997)
Published Online	27 years, 9 months ago (Nov. 17, 1997)
Published Print	28 years, 2 months ago (June 30, 1997)

Funders 0

None

BibTeX

@article{Eto_1997, title={Molecular cloning of a novel phosphorylation‐dependent inhibitory protein of protein phosphatase‐1 (CPI17) in smooth muscle: its specific localization in smooth muscle1}, volume={410}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)00657-1}, DOI={10.1016/s0014-5793(97)00657-1}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Eto, Masumi and Senba, Shingo and Morita, Fumi and Yazawa, Michio}, year={1997}, month=jun, pages={356–360} }

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