DOI: 10.1016/s0014-5793(97)00462-6. Postimport methylation of the small subunit of ribulose‐1,5‐bisphosphate carboxylase in chloroplasts

Postimport methylation of the small subunit of ribulose‐1,5‐bisphosphate carboxylase in chloroplasts

10.1016/s0014-5793(97)00462-6

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Electron impact mass spectronomy analysis of the amino‐terminal amino acid of the small subunit (SSU) of ribulose‐1,5‐bisphosphate carboxylase (Rubisco) showed that the amino‐terminal methionine residue is post‐translationally modified to N‐methyl‐methionine. Modification of the amino‐terminal methionine residue was found in mature SSU proteins from the dicotyledonous plants pea and spinach as well as the monocotyledonous plants barley and corn. SSU methyltransferase is a soluble protein in the chloroplast stroma and accepts heterologously expressed non‐methylated SSU as a substrate using S‐adenosylmethionine as methyl‐group donor. We show that this modification occurs after post‐translational uptake of the precursor form of SSU into chloroplasts and processing to its mature size. This reaction represents a new step in the import and assembly pathway of Rubisco holoenzyme.

Bibliography

Grimm, R., Grimm, M., Eckerskorn, C., Pohlmeyer, K., RÃ¶hl, T., & Soll, J. (1997). Postimport methylation of the small subunit of ribuloseâ1,5âbisphosphate carboxylase in chloroplasts. FEBS Letters, 408(3), 350â354. Portico.

Authors 6

Rudolf Grimm (first)
Monika Grimm (additional)
Christoph Eckerskorn (additional)
Kai Pohlmeyer (additional)
Thomas Röhl (additional)
Jürgen Soll (additional)

References 25 Referenced 38

10.1038/287692a0
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10.1002/j.1460-2075.1982.tb01345.x
10.1073/pnas.75.12.6110
10.1111/j.1432-1033.1984.tb08291.x
10.1007/BF00039488
10.1073/pnas.82.15.5083
A.R. Cashmore in: T. Kosuge C.P. Meredith A. Hollaender (Eds.) Genetic engineering of plants Plenum Press New York 1983 pp. 29-38.
10.1016/S0031-9422(00)84156-4
10.1111/j.1365-313X.1991.00149.x
10.1016/S0091-679X(08)61035-3
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10.1073/pnas.85.5.1513
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{'key': 'e_1_2_6_25_1', 'first-page': '201', 'volume': '231', 'author': 'Stock A.', 'year': '1988', 'journal-title': 'Adv. Exp. Med. Biol.'} / Adv. Exp. Med. Biol. by Stock A. (1988)
{'key': 'e_1_2_6_26_1', 'first-page': '285', 'volume': '21', 'author': 'Keeling P.J.', 'year': '1996', 'journal-title': 'TIBS'} / TIBS by Keeling P.J. (1996)

Dates

Type	When
Created	22 years, 10 months ago (Oct. 11, 2002, 6:59 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:19 a.m.)
Indexed	4 weeks ago (Aug. 6, 2025, 9:55 a.m.)
Issued	28 years, 3 months ago (May 26, 1997)
Published	28 years, 3 months ago (May 26, 1997)
Published Online	27 years, 10 months ago (Oct. 28, 1997)
Published Print	28 years, 3 months ago (May 26, 1997)

Funders 0

None

BibTeX

@article{Grimm_1997, title={Postimport methylation of the small subunit of ribulose‐1,5‐bisphosphate carboxylase in chloroplasts}, volume={408}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)00462-6}, DOI={10.1016/s0014-5793(97)00462-6}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Grimm, Rudolf and Grimm, Monika and Eckerskorn, Christoph and Pohlmeyer, Kai and Röhl, Thomas and Soll, Jürgen}, year={1997}, month=may, pages={350–354} }

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