DOI: 10.1016/s0014-5793(97)00386-4. Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co‐factor for epidermal growth factor in cell culture

Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co‐factor for epidermal growth factor in cell culture

10.1016/s0014-5793(97)00386-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Oligosaccharides, involved in regulation of plant developmental and defensive processes, were tested to determine their ability to enhance proliferation of human keratinocytes. A mixture of alginate oligosaccharides remarkably stimulated keratinocyte growth and [3H]thymidine uptake in the presence of epidermal growth factor (EGF). The activity was comparable to bovine pituitary extract, a common complement in keratinocyte culture, and additive on BPE‐induced stimulation. The most effective oligosaccharide in the mixture was identified and its chemical structure was determined. These findings demonstrate a novel activity of alginate oligosaccharide(s) in keratinocyte growth and suggest a possible co‐factor for EGF‐dependent stimulation in medium for keratinocytes.

Bibliography

Kawada, A., Hiura, N., Shiraiwa, M., Tajima, S., Hiruma, M., Hara, K., Ishibashi, A., & Takahara, H. (1997). Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible coâfactor for epidermal growth factor in cell culture. FEBS Letters, 408(1), 43â46. Portico.

Authors 8

Akira Kawada (first)
Nozomi Hiura (additional)
Masakazu Shiraiwa (additional)
Shingo Tajima (additional)
Masataro Hiruma (additional)
Kenji Hara (additional)
Akira Ishibashi (additional)
Hidenari Takahara (additional)

References 22 Referenced 97

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:54 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 11:18 p.m.)
Indexed	2 months, 1 week ago (June 17, 2025, 12:54 a.m.)
Issued	28 years, 3 months ago (May 12, 1997)
Published	28 years, 3 months ago (May 12, 1997)
Published Online	27 years, 9 months ago (Nov. 3, 1997)
Published Print	28 years, 3 months ago (May 12, 1997)

Funders 0

None

BibTeX

@article{Kawada_1997, title={Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co‐factor for epidermal growth factor in cell culture}, volume={408}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(97)00386-4}, DOI={10.1016/s0014-5793(97)00386-4}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Kawada, Akira and Hiura, Nozomi and Shiraiwa, Masakazu and Tajima, Shingo and Hiruma, Masataro and Hara, Kenji and Ishibashi, Akira and Takahara, Hidenari}, year={1997}, month=may, pages={43–46} }

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