DOI: 10.1016/s0014-5793(96)01180-5. Exogenously injected nuclear import factor p10/NTF2 inhibits signal‐mediated nuclear import and export of proteins in living cells

Exogenously injected nuclear import factor p10/NTF2 inhibits signal‐mediated nuclear import and export of proteins in living cells

10.1016/s0014-5793(96)01180-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

p10/NTF2 is a cytosolic factor which is required for the translocation step in nuclear protein import in an in vitro assay with digitonin‐permeabilized cells. To study the functional roles of p10/NTF2 on protein transport between the nucleus and cytoplasm in living cells, recombinant p10/NTF2 was microinjected injo cultured mammalian cells. Cytoplasmically injected p10/NTF2 strongly inhibited the nuclear import of co‐injected NLS‐containing substrates in a dose‐dependent manner but had no effect on the diffusive import of small non‐nuclear proteins. Moreover, when injected into the cell nucleus, p10/NTF2 inhibited the nuclear export of NES‐containing substrates. The results suggest that the nuclear import factor p10/NTF2 may also be involved in the nuclear export of proteins and that the protein transport efficiency between the nucleus and cytoplasm may be regulated by the intracellular level of p10/NTF2.

Bibliography

Tachibana, T., Hieda, M., Sekimoto, T., & Yoneda, Y. (1996). Exogenously injected nuclear import factor p10/NTF2 inhibits signalâmediated nuclear import and export of proteins in living cells. FEBS Letters, 397(2â3), 177â182. Portico.

Authors 4

Taro Tachibana (first)
Miki Hieda (additional)
Toshihiro Sekimoto (additional)
Yoshihiro Yoneda (additional)

References 37 Referenced 45

10.1016/0092-8674(88)90402-3
10.1016/0092-8674(88)90403-5
10.1016/0092-8674(92)90613-H
10.1016/0955-0674(95)80084-0
10.1083/jcb.119.5.1047
10.1128/MCB.12.5.2186
10.1006/excr.1993.1129
10.1074/jbc.270.15.8559
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10.1002/j.1460-2075.1995.tb00031.x
1995 J. Moroianu G. Blobel A. Radu Proc. Natl. Acad. Sci. USA 2008 2011
10.1126/science.7754385
10.1083/jcb.125.3.547
10.1016/0014-5793(95)00699-A
10.1016/S0960-9822(95)00079-0
1995 J. Moroianu M. Hijikata G. Blobel A. Radu Proc. Natl. Acad. Sci. USA 6532 6536
10.1038/365661a0
10.1083/jcb.123.6.1649
1994 M.S. Moore G. Blobel Proc. Natl. Acad. Sci. USA 10212 10216
10.1016/S0021-9258(17)31424-2
10.1083/jcb.129.4.925
10.1016/0092-8674(95)90435-2
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10.1016/0092-8674(95)90438-7
10.1016/0966-842X(96)81494-6
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10.1016/0014-4827(87)90319-3
10.1016/S0076-6879(95)57032-2
10.1083/jcb.111.3.807
1995 J. Moroianu G. Blobel Proc. Nat]. Acad. Sci. USA 4318 4322
10.1016/0092-8674(95)90323-2
1995 G. Schlenstedt C. Saavedra J.D. Loeb C.N. Cole P.A. Silver Proc. Natl. Acad. Sci. USA 225 229
10.1016/0092-8674(90)90348-I
10.1126/science.272.5258.120

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 8:19 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 9:52 p.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 9:50 a.m.)
Issued	28 years, 9 months ago (Nov. 18, 1996)
Published	28 years, 9 months ago (Nov. 18, 1996)
Published Online	25 years, 8 months ago (Dec. 2, 1999)
Published Print	28 years, 9 months ago (Nov. 18, 1996)

Funders 0

None

BibTeX

@article{Tachibana_1996, title={Exogenously injected nuclear import factor p10/NTF2 inhibits signal‐mediated nuclear import and export of proteins in living cells}, volume={397}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(96)01180-5}, DOI={10.1016/s0014-5793(96)01180-5}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Tachibana, Taro and Hieda, Miki and Sekimoto, Toshihiro and Yoneda, Yoshihiro}, year={1996}, month=nov, pages={177–182} }

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