DOI: 10.1016/s0014-5793(96)01127-1. Topographical structure of membrane‐bound Escherichia coli F1F0 ATP synthase in aqueous buffer

Topographical structure of membrane‐bound Escherichia coli F1F0 ATP synthase in aqueous buffer

10.1016/s0014-5793(96)01127-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Scanning force microscope images of membrane‐bound Escherichia coli ATP synthase F0 complexes have been obtained in aqueous solution. The images show a consistent set of internal features: a ring structure which surrounds a central dimple and contains an asymmetric lateral mass. Images of trypsin‐treated F0 complexes, which have lost part of their b subunits, show a reduced asymmetric mass, while images of c‐subunit oligomers, which lack both the a and b subunits, show a ring and dimple but do not have an asymmetric mass. These results support models in which the F0 complex contains a ring of 9–12 c subunits with the b subunits located outside this ring, and show that scanning force microscopy is able to provide structural information on membrane proteins of molecular mass less than 200 000 Da.

Bibliography

Singh, S., Turina, P., Bustamante, C. J., Keller, D. J., & Capaldi, R. (1996). Topographical structure of membraneâbound Escherichia coli F1F0 ATP synthase in aqueous buffer. FEBS Letters, 397(1), 30â34. Portico.

Authors 5

Seema Singh (first)
Paola Turina (additional)
Carlos J. Bustamante (additional)
David J. Keller (additional)
Roderick Capaldi (additional)

References 36 Referenced 105

10.1016/0968-0004(94)90006-X
10.1146/annurev.bb.19.060190.000255
1990 New York Academy Press New York R.H. Fillingame T.A. Krulwich The Bacteria: A Treatise on Structure and Function 345 391
10.1021/bi00474a019
10.1038/370621a0
10.1038/nsb1195-961
10.1515/znc-1990-7-806 / Z. Naturforsch. by Berzborn R.J. (1990)
10.1021/bi00236a007
10.1242/jeb.172.1.451
10.1016/0005-2728(86)90096-4
10.1063/1.881478
1994 S. Karrasch R. Hegerl J.H. Hoh W. Baumeister A. Engel Proc. Natl. Acad. Sci USA 836 838
10.1006/jmbi.1993.1033
10.1083/jcb.122.5.977
10.1006/jmbi.1995.0238
10.1016/S0021-9258(19)70240-3
10.1021/bi00396a036
1978 F. Szoka D. Papahadjopoulos Proc. Natl. Acad Sci. USA 4194 4198
10.1111/j.1432-1033.1990.tb19418.x
10.1021/bi00313a018
10.1016/S0021-9258(17)44568-6
10.1042/bj1250169
1973 M.F. Sierra A. Tzagaloff Proc. Natl. Acad. Sci. 3155 3159
10.1016/S0021-9258(17)32992-7
10.1063/1.111795
10.1016/0039-6028(92)90973-A
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10.1128/mr.51.4.477-497.1987
10.1111/j.1432-1033.1993.tb17730.x
10.1111/j.1432-1033.1988.tb13743.x
{'key': 'e_1_2_1_34_1', 'first-page': '187', 'volume': '6', 'author': 'Van Heel M.', 'year': '1981', 'journal-title': 'Ultramicroscopy'} / Ultramicroscopy by Van Heel M. (1981)
10.1515/bchm3.1994.375.1.43
10.1016/0005-2728(93)90063-L
10.1016/S0021-9258(18)43822-7

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:54 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 9:10 p.m.)
Indexed	3 weeks ago (Aug. 6, 2025, 8:10 a.m.)
Issued	28 years, 9 months ago (Nov. 11, 1996)
Published	28 years, 9 months ago (Nov. 11, 1996)
Published Online	25 years, 8 months ago (Dec. 2, 1999)
Published Print	28 years, 9 months ago (Nov. 11, 1996)

Funders 0

None

BibTeX

@article{Singh_1996, title={Topographical structure of membrane‐bound Escherichia coli F1F0 ATP synthase in aqueous buffer}, volume={397}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(96)01127-1}, DOI={10.1016/s0014-5793(96)01127-1}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Singh, Seema and Turina, Paola and Bustamante, Carlos J. and Keller, David J. and Capaldi, Roderick}, year={1996}, month=nov, pages={30–34} }

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