DOI: 10.1016/s0014-5793(04)00221-2. Formation of circular polyribosomes in wheat germ cell‐free protein synthesis system

Formation of circular polyribosomes in wheat germ cell‐free protein synthesis system

10.1016/s0014-5793(04)00221-2

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

We report a morphological study of functioning ribosomes in a efficient and robust cell‐free protein synthesis system prepared from wheat embryos. Sucrose density gradient analysis of translated mixtures programmed with luciferase mRNAs having different 5′ and 3′ untranslated regions showed formation of large polysomes. Electron microscopic examination of translation mixtures programmed with those of capped and polyadenylated mRNA revealed that ribosomes assemble into a circular‐type polysome in vitro. Furthermore, a series of experiments using mRNAs lacking either cap, poly(A) tail or both also resulted in the formation of circular polysomes, which are indistinguishable from those with the original mRNA. The wheat germ cell‐free system may provide a good experimental system for understanding functional ribosomes at the molecular level.

Bibliography

Madin, K., Sawasaki, T., Kamura, N., Takai, K., Ogasawara, T., Yazaki, K., Takei, T., Miura, K.-I., & Endo, Y. (2004). Formation of circular polyribosomes in wheat germ cellâfree protein synthesis system. FEBS Letters, 562(1â3), 155â159. Portico.

Authors 9

Kairat Madin (first)
Tatsuya Sawasaki (additional)
Nami Kamura (additional)
Kazuyuki Takai (additional)
Tomio Ogasawara (additional)
Kazumori Yazaki (additional)
Toshiaki Takei (additional)
Kin-Ichiro Miura (additional)
Yaeta Endo (additional)

References 29 Referenced 30

Spirin A.S. (1990) in: The Ribosome: Structure Function and Evolution Vol. 2 (Hill E.H. Dahlberg A. Garrot R.A. Moore P.B. Schlessinger D. and Warner J.R. Eds.) pp. 56–70 American Society for Microbiology Washington DC.
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Frank J. Penczek P. Grassuci R.A. Heagle A. Spahn C.M.T. and Agrawal R.K. (2000) in: The Ribosome: Structure Function and Cellular Interactions (Garrett R.A. Douthwaite S.R. Liljas A. Matheson A.T. Moore P.B. and Noller H.F. Eds.) pp. 45–51 American Society for Microbiology Washington DC. (10.1128/9781555818142.ch5)
{'key': 'e_1_2_4_20_1', 'first-page': '81', 'volume': '1', 'author': 'Sundaralingam M.', 'year': '1974', 'journal-title': 'Int. J. Quantum Chem. Quantum Biol. Symp.'} / Int. J. Quantum Chem. Quantum Biol. Symp. by Sundaralingam M. (1974)
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10.1126/science.271.5251.1000
10.1126/science.1060089

Dates

Type	When
Created	21 years, 5 months ago (March 6, 2004, 5:39 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:11 a.m.)
Indexed	4 months, 2 weeks ago (April 8, 2025, 6:59 p.m.)
Issued	21 years, 5 months ago (March 5, 2004)
Published	21 years, 5 months ago (March 5, 2004)
Published Online	21 years, 5 months ago (March 5, 2004)
Published Print	21 years, 4 months ago (March 26, 2004)

Funders 0

None

BibTeX

@article{Madin_2004, title={Formation of circular polyribosomes in wheat germ cell‐free protein synthesis system}, volume={562}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(04)00221-2}, DOI={10.1016/s0014-5793(04)00221-2}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={Madin, Kairat and Sawasaki, Tatsuya and Kamura, Nami and Takai, Kazuyuki and Ogasawara, Tomio and Yazaki, Kazumori and Takei, Toshiaki and Miura, Kin-Ichiro and Endo, Yaeta}, year={2004}, month=mar, pages={155–159} }

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