DOI: 10.1016/s0014-5793(04)00052-3. Low temperature or GroEL/ES overproduction permits growth of <i>Escherichia coli</i> cells lacking trigger factor and DnaK

Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK

10.1016/s0014-5793(04)00052-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Escherichia coli trigger factor (TF) and DnaK cooperate in the folding of newly synthesized proteins. The combined deletion of the TF‐encoding tig gene and the dnaK gene causes protein aggregation and synthetic lethality at 30°C. Here we show that the synthetic lethality of ΔtigΔdnaK52 cells is abrogated either by growth below 30°C or by overproduction of GroEL/GroES. At 23°C ΔtigΔdnaK52 cells were viable and showed only minor protein aggregation. Overproduction of GroEL/GroES, but not of other chaperones, restored growth of ΔtigΔdnaK52 cells at 30°C and suppressed protein aggregation including proteins ≥60 kDa, which normally require TF and DnaK for folding. GroEL/GroES thus influences the folding of proteins previously identified as DnaK/TF substrates.

Bibliography

VorderwÃ¼lbecke, S., Kramer, G., Merz, F., Kurz, T. A., Rauch, T., Zachmann-Brand, B., Bukau, B., & Deuerling, E. (2004). Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK. FEBS Letters, 559(1â3), 181â187. Portico.

Authors 8

S Vorderwülbecke (first)
G Kramer (additional)
F Merz (additional)
T.A Kurz (additional)
T Rauch (additional)
B Zachmann-Brand (additional)
B Bukau (additional)
E Deuerling (additional)

References 25 Referenced 92

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Dates

Type	When
Created	21 years, 7 months ago (Jan. 27, 2004, 5:22 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 10:12 a.m.)
Indexed	3 months, 3 weeks ago (May 10, 2025, 2:21 p.m.)
Issued	21 years, 7 months ago (Jan. 24, 2004)
Published	21 years, 7 months ago (Jan. 24, 2004)
Published Online	21 years, 7 months ago (Jan. 24, 2004)
Published Print	21 years, 6 months ago (Feb. 13, 2004)

Funders 0

None

BibTeX

@article{Vorderw_lbecke_2004, title={Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK}, volume={559}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(04)00052-3}, DOI={10.1016/s0014-5793(04)00052-3}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={Vorderwülbecke, S and Kramer, G and Merz, F and Kurz, T.A and Rauch, T and Zachmann-Brand, B and Bukau, B and Deuerling, E}, year={2004}, month=jan, pages={181–187} }

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