DOI: 10.1016/s0014-5793(03)01453-4. Cation‐selective ion channels formed by p7 of hepatitis C virus are blocked by hexamethylene amiloride

Cation‐selective ion channels formed by p7 of hepatitis C virus are blocked by hexamethylene amiloride

10.1016/s0014-5793(03)01453-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

A 63 residue peptide, p7, encoded by hepatitis C virus was synthesised and tested for ion channel activity in lipid bilayer membranes. Ion channels formed by p7 had a variable conductance: some channels had conductances as low as 14 pS. The reversal potential of currents flowing through the channels formed by p7 showed that they were permeable to potassium and sodium ions and less permeable to calcium ions. Addition of Ca2+ to solutions made channels formed by p7 less potassium‐ or sodium‐selective. Hexamethylene amiloride, a drug previously shown to block ion channels formed by Vpu encoded by HIV‐1, blocked channels formed by p7. In view of the increasing number of peptides encoded by viruses that have been shown to form ion channels, it is suggested that ion channels may play an important role in the life cycle of many viruses and that drugs that block these channels may prove to be useful antiviral agents.

Bibliography

Premkumar, A., Wilson, L., Ewart, G. D., & Gage, P. W. (2003). Cationâselective ion channels formed by p7 of hepatitis C virus are blocked by hexamethylene amiloride. FEBS Letters, 557(1â3), 99â103. Portico.

Authors 4

A Premkumar (first)
L Wilson (additional)
G.D Ewart (additional)
P.W Gage (additional)

References 23 Referenced 168

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Dates

Type	When
Created	21 years, 8 months ago (Dec. 19, 2003, 5:58 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 2:22 a.m.)
Indexed	2 weeks, 5 days ago (Aug. 6, 2025, 8:48 a.m.)
Issued	21 years, 8 months ago (Dec. 16, 2003)
Published	21 years, 8 months ago (Dec. 16, 2003)
Published Online	21 years, 8 months ago (Dec. 16, 2003)
Published Print	21 years, 7 months ago (Jan. 16, 2004)

Funders 0

None

BibTeX

@article{Premkumar_2003, title={Cation‐selective ion channels formed by p7 of hepatitis C virus are blocked by hexamethylene amiloride}, volume={557}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(03)01453-4}, DOI={10.1016/s0014-5793(03)01453-4}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={Premkumar, A and Wilson, L and Ewart, G.D and Gage, P.W}, year={2003}, month=dec, pages={99–103} }

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