DOI: 10.1016/s0014-5793(03)00705-1. Differential association of the auxiliary subunit Kvβ2 with Kv1.4 and Kv4.3 K<sup>+</sup> channels

Differential association of the auxiliary subunit Kvβ2 with Kv1.4 and Kv4.3 K+ channels

10.1016/s0014-5793(03)00705-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Kvβ2 subunits associate with Kv1 and Kv4 K+ channels, but the basis of preferential association is not understood. For example, detergent resistance suggests stronger auxiliary subunit association with Kv4.2 than with Kv1.2, but Kvβ2 preferentially localizes with the latter channels in brain. Here we examine the interaction of Kvβ2 with two native binding partners in brain: Kv4.3 and Kv1.4. We show that the auxiliary subunit binds more efficiently to Kv1.4 than to Kv4.3 in mammalian cells. However, preexisting Kvβ2 complexes with Kv1.4 and Kv4.3 have similar detergent sensitivity. Thus, preferential steady state binding may reflect a difference in initial association rather than stability. We also find that that the cytoplasmic C‐terminus of Kv4.3 inhibits Kvβ2 association. Apparently, a region proximal to the Kv4.3 pore contributes to the inefficient auxiliary subunit interaction that produces preferential binding of Kvβ2 to Kv1 channels.

Bibliography

Wang, L., Takimoto, K., & Levitan, E. S. (2003). Differential association of the auxiliary subunit KvÎ²2 with Kv1.4 and Kv4.3 K+ channels. FEBS Letters, 547(1â3), 162â164. Portico.

Authors 3

Lin Wang (first)
Koichi Takimoto (additional)
Edwin S Levitan (additional)

References 14 Referenced 10

Dates

Type	When
Created	22 years, 1 month ago (June 30, 2003, 12:35 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 11:59 p.m.)
Indexed	1 year, 3 months ago (May 8, 2024, 10:57 p.m.)
Issued	22 years, 2 months ago (June 24, 2003)
Published	22 years, 2 months ago (June 24, 2003)
Published Online	22 years, 2 months ago (June 24, 2003)
Published Print	22 years, 1 month ago (July 17, 2003)

Funders 0

None

BibTeX

@article{Wang_2003, title={Differential association of the auxiliary subunit Kvβ2 with Kv1.4 and Kv4.3 K+ channels}, volume={547}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(03)00705-1}, DOI={10.1016/s0014-5793(03)00705-1}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={Wang, Lin and Takimoto, Koichi and Levitan, Edwin S}, year={2003}, month=jun, pages={162–164} }

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