DOI: 10.1016/s0014-5793(03)00521-0. Spectral imaging and its applications in live cell microscopy

Spectral imaging and its applications in live cell microscopy

10.1016/s0014-5793(03)00521-0

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

In biological microscopy, the ever expanding range of applications requires quantitative approaches that analyze several distinct fluorescent molecules at the same time in the same sample. However, the spectral properties of the fluorescent proteins and dyes presently available set an upper limit to the number of molecules that can be detected simultaneously with common microscopy methods. Spectral imaging and linear unmixing extends the possibilities to discriminate distinct fluorophores with highly overlapping emission spectra and thus the possibilities of multicolor imaging. This method also offers advantages for fast multicolor time‐lapse microscopy and fluorescence resonance energy transfer measurements in living samples. Here we discuss recent progress on the technical implementation of the method, its limitations and applications to the imaging of biological samples.

Bibliography

Zimmermann, T., Rietdorf, J., & Pepperkok, R. (2003). Spectral imaging and its applications in live cell microscopy. FEBS Letters, 546(1), 87â92. Portico.

Authors 3

Timo Zimmermann (first)
Jens Rietdorf (additional)
Rainer Pepperkok (additional)

References 26 Referenced 439

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Dates

Type	When
Created	22 years, 3 months ago (May 19, 2003, 1:32 p.m.)
Deposited	1 year, 11 months ago (Sept. 12, 2023, 2:23 p.m.)
Indexed	8 hours, 37 minutes ago (Aug. 27, 2025, 12:13 p.m.)
Issued	22 years, 3 months ago (May 17, 2003)
Published	22 years, 3 months ago (May 17, 2003)
Published Online	22 years, 3 months ago (May 17, 2003)
Published Print	22 years, 1 month ago (July 3, 2003)

Funders 0

None

BibTeX

@article{Zimmermann_2003, title={Spectral imaging and its applications in live cell microscopy}, volume={546}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(03)00521-0}, DOI={10.1016/s0014-5793(03)00521-0}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Zimmermann, Timo and Rietdorf, Jens and Pepperkok, Rainer}, year={2003}, month=may, pages={87–92} }

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