DOI: 10.1016/s0014-5793(02)03069-7. Topology determination and functional analysis of the <i>Escherichia coli</i> TatC protein

Topology determination and functional analysis of the Escherichia coli TatC protein

10.1016/s0014-5793(02)03069-7

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The TatC protein is an essential component of the bacterial Tat system. By using alkaline phosphatase and β‐glucuronidase fusions we found that TatC contains four transmembrane helices. Three insertions of Ala‐Ser dipeptide at the cytoplasmic N‐ and C‐termini and in the cytoplasmic loop had no or only partial effect on the TatC function. In contrast, five of seven insertions in the two periplasmic loops abolished the Tat function. Four insertions analyzed had no effect on the stability of the altered TatC proteins or on membrane assembly of the TatA and TatB proteins. These data provide a novel base for more detailed studies of the mechanism of the Tat system.

Bibliography

Gouffi, K., Santini, C.-L., & Wu, L.-F. (2002). Topology determination and functional analysis of the Escherichia coli TatC protein. FEBS Letters, 525(1â3), 65â70. Portico.

Authors 3

Kamila Gouffi (first)
Claire-Lise Santini (additional)
Long-Fei Wu (additional)

References 30 Referenced 42

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{'key': 'e_1_2_6_3_1', 'first-page': '179', 'volume': '2', 'author': 'Wu L.-F.', 'year': '2000', 'journal-title': 'J. Mol. Microbiol. Biotechnol'} / J. Mol. Microbiol. Biotechnol by Wu L.-F. (2000)
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Dates

Type	When
Created	22 years, 10 months ago (Oct. 14, 2002, 11:56 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 1:48 a.m.)
Indexed	1 year, 4 months ago (April 7, 2024, 1:17 a.m.)
Issued	23 years, 1 month ago (July 16, 2002)
Published	23 years, 1 month ago (July 16, 2002)
Published Online	23 years, 1 month ago (July 16, 2002)
Published Print	23 years ago (Aug. 14, 2002)

Funders 0

None

BibTeX

@article{Gouffi_2002, title={Topology determination and functional analysis of the Escherichia coli TatC protein}, volume={525}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(02)03069-7}, DOI={10.1016/s0014-5793(02)03069-7}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={Gouffi, Kamila and Santini, Claire-Lise and Wu, Long-Fei}, year={2002}, month=jul, pages={65–70} }

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