DOI: 10.1016/s0014-5793(01)03278-1. Components required for membrane assembly of newly synthesized K<sup>+</sup> channel KcsA

Components required for membrane assembly of newly synthesized K+ channel KcsA

10.1016/s0014-5793(01)03278-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

An Escherichia coli in vitro transcription–translation system was used to study the components involved in the biogenesis of the homotetrameric potassium channel KcsA. We show that a functional signal recognition particle pathway is essential for tetramer formation, probably to direct correct monomer insertion in the membrane. In the absence of YidC or at reduced SecYEG levels, KcsA assembly occurs with lower efficiency. Strikingly, the highest efficiency of tetramerization was observed when transcription–translation was carried out in the presence of pure lipid vesicles, demonstrating that a phospholipid bilayer is the minimal membrane requirement to form the KcsA tetramer. It is concluded that SecYEG and YidC are not required for the formation of tetrameric KcsA in vitro.

Bibliography

van Dalen, A., van der Laan, M., Driessen, A. J. M., Killian, J. A., & de Kruijff, B. (2001). Components required for membrane assembly of newly synthesized K+ channel KcsA. FEBS Letters, 511(1â3), 51â58. Portico.

Authors 5

Annemieke van Dalen (first)
Martin van der Laan (additional)
Arnold J.M. Driessen (additional)
J.Antoinette Killian (additional)
Ben de Kruijff (additional)

References 50 Referenced 25

Dates

Type	When
Created	22 years, 10 months ago (Oct. 14, 2002, 11:56 a.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 8:26 p.m.)
Indexed	1 year, 6 months ago (Feb. 23, 2024, 1:28 p.m.)
Issued	23 years, 8 months ago (Dec. 20, 2001)
Published	23 years, 8 months ago (Dec. 20, 2001)
Published Online	23 years, 8 months ago (Dec. 20, 2001)
Published Print	23 years, 6 months ago (Jan. 30, 2002)

Funders 0

None

BibTeX

@article{van_Dalen_2001, title={Components required for membrane assembly of newly synthesized K+ channel KcsA}, volume={511}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(01)03278-1}, DOI={10.1016/s0014-5793(01)03278-1}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={van Dalen, Annemieke and van der Laan, Martin and Driessen, Arnold J.M. and Killian, J.Antoinette and de Kruijff, Ben}, year={2001}, month=dec, pages={51–58} }

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