DOI: 10.1016/s0014-5793(01)03163-5. Phosphatidylglycerophosphate synthases from <i>Arabidopsis thaliana</i>

Phosphatidylglycerophosphate synthases from Arabidopsis thaliana

10.1016/s0014-5793(01)03163-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Two Arabidopsis thaliana genes were shown to encode phosphatidylglycerophosphate synthases (PGPS) of 25.4 and 32.2 kDa, respectively. Apart from their N‐terminal regions, the two proteins exhibit high sequence similarity. Functional expression studies in yeast provided evidence that the 25.4 kDa protein is a microsomal PGPS while the 32.2 kDa protein represents a preprotein which can be imported into yeast mitochondria and processed to a mature PGPS. The two isozymes were solubilized and purified as fusion proteins carrying a His tag at their C‐terminus. Enzyme assays with both membrane fractions and purified enzyme fractions revealed that the two A. thaliana isozymes have similar properties but differ in their CDP‐diacylglycerol species specificity.

Bibliography

MÃ¼ller, F., & Frentzen, M. (2001). Phosphatidylglycerophosphate synthases from Arabidopsis thaliana. FEBS Letters, 509(2), 298â302. Portico.

Authors 2

Frank Müller (first)
Margrit Frentzen (additional)

References 25 Referenced 52

10.1046/j.1365-2958.2001.02320.x
10.1073/pnas.97.19.10655
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10.1074/jbc.273.16.9829
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10.1016/S0005-2760(97)00116-1
10.1146/annurev.pp.33.060182.001315
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10.1128/jb.178.4.1030-1038.1996
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Hagio M. Sakurai J. Gombos Z. and Wada H. (2001) NPLC Meeting in South Lake Tahoe Abstract P4 National Plant Lipid Cooperative South Lake Tahoe.
10.1021/bi00669a002
10.1128/jb.142.1.262-267.1980

Dates

Type	When
Created	22 years, 10 months ago (Oct. 14, 2002, 11:56 a.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 10:10 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 6, 2025, 8:11 a.m.)
Issued	23 years, 9 months ago (Nov. 27, 2001)
Published	23 years, 9 months ago (Nov. 27, 2001)
Published Online	23 years, 9 months ago (Nov. 27, 2001)
Published Print	23 years, 8 months ago (Dec. 7, 2001)

Funders 0

None

BibTeX

@article{M_ller_2001, title={Phosphatidylglycerophosphate synthases from Arabidopsis thaliana}, volume={509}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(01)03163-5}, DOI={10.1016/s0014-5793(01)03163-5}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Müller, Frank and Frentzen, Margrit}, year={2001}, month=nov, pages={298–302} }

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