DOI: 10.1016/s0014-5793(01)03134-9. Identification of mouse <i>trp</i> homologs and lipid rafts from spermatogenic cells and sperm

Identification of mouse trp homologs and lipid rafts from spermatogenic cells and sperm

10.1016/s0014-5793(01)03134-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Intracellular Ca2+ has an important regulatory role in the control of sperm motility, capacitation, and the acrosome reaction (AR). However, little is known about the molecular identity of the membrane systems that regulate Ca2+ in sperm. In this report, we provide evidence for the expression of seven Drosophila transient receptor potential homolog genes (trp1–7) and three of their protein products (Trp1, Trp3 and Trp6) in mouse sperm. Allegedly some trps encode capacitative Ca2+ channels. Immunoconfocal images showed that while Trp6 was present in the postacrosomal region and could be involved in sperm AR, expression of Trp1 and Trp3 was confined to the flagellum, suggesting that they may serve sperm to regulate important Ca2+‐dependent events in addition to the AR. Likewise, one of these proteins (Trp1) co‐immunolocalized with caveolin‐1, a major component of caveolae, a subset of lipid rafts potentially important for signaling events and Ca2+ flux. Furthermore, by using fluorescein‐coupled cholera toxin B subunit, which specifically binds to the raft component ganglioside GM1, we identified caveolin‐ and Trp‐independent lipid rafts residing in the plasma membrane of mature sperm. Notably, the distribution of GM1 changes drastically upon completion of the AR.

Bibliography

TreviÃ±o, C. L., Serrano, C. J., BeltrÃ¡n, C., Felix, R., & Darszon, A. (2001). Identification of mouse trp homologs and lipid rafts from spermatogenic cells and sperm. FEBS Letters, 509(1), 119â125. Portico.

Authors 5

Claudia L. Treviño (first)
Carmen J. Serrano (additional)
Carmen Beltrán (additional)
Ricardo Felix (additional)
Alberto Darszon (additional)

References 33 Referenced 145

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Dates

Type	When
Created	22 years, 10 months ago (Oct. 14, 2002, 11:56 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 8:29 a.m.)
Indexed	3 months, 1 week ago (May 27, 2025, 1:45 a.m.)
Issued	23 years, 9 months ago (Nov. 27, 2001)
Published	23 years, 9 months ago (Nov. 27, 2001)
Published Online	23 years, 9 months ago (Nov. 27, 2001)
Published Print	23 years, 9 months ago (Nov. 30, 2001)

Funders 0

None

BibTeX

@article{Trevi_o_2001, title={Identification of mouse trp homologs and lipid rafts from spermatogenic cells and sperm}, volume={509}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(01)03134-9}, DOI={10.1016/s0014-5793(01)03134-9}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Treviño, Claudia L. and Serrano, Carmen J. and Beltrán, Carmen and Felix, Ricardo and Darszon, Alberto}, year={2001}, month=nov, pages={119–125} }

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