DOI: 10.1016/s0014-5793(01)03115-5. α‐Synuclein metabolism and aggregation is linked to ubiquitin‐independent degradation by the proteasome

α‐Synuclein metabolism and aggregation is linked to ubiquitin‐independent degradation by the proteasome

10.1016/s0014-5793(01)03115-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

α‐Synuclein has been implicated in the pathogenesis of Parkinson's disease based on mutations in familial cases of the disease and its presence in Lewy bodies. Here we show that over‐expression of wild‐type human α‐synuclein is sufficient to induce inclusion formation in SH‐SY5Y cells. In this cellular model, proteasome inhibition leads to an increase of α‐synuclein accumulation in vivo without ubiquitylation. In accordance, we find that in vitro, unmodified α‐synuclein can be directly degraded by the 20S proteasome. These findings suggest an ubiquitin‐independent mechanism of proteasomal degradation for α‐synuclein and other natively unfolded proteins.

Bibliography

Tofaris, G. K., Layfield, R., & Spillantini, M. G. (2001). Î±âSynuclein metabolism and aggregation is linked to ubiquitinâindependent degradation by the proteasome. FEBS Letters, 509(1), 22â26. Portico.

Authors 3

George K Tofaris (first)
Robert Layfield (additional)
Maria Grazia Spillantini (additional)

References 32 Referenced 295

Dates

Type	When
Created	22 years, 10 months ago (Oct. 14, 2002, 11:56 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 8:29 a.m.)
Indexed	4 weeks, 1 day ago (Aug. 2, 2025, 1:09 a.m.)
Issued	23 years, 9 months ago (Nov. 9, 2001)
Published	23 years, 9 months ago (Nov. 9, 2001)
Published Online	23 years, 9 months ago (Nov. 9, 2001)
Published Print	23 years, 9 months ago (Nov. 30, 2001)

Funders 0

None

BibTeX

@article{Tofaris_2001, title={α‐Synuclein metabolism and aggregation is linked to ubiquitin‐independent degradation by the proteasome}, volume={509}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(01)03115-5}, DOI={10.1016/s0014-5793(01)03115-5}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Tofaris, George K and Layfield, Robert and Spillantini, Maria Grazia}, year={2001}, month=nov, pages={22–26} }

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