DOI: 10.1016/s0014-5793(01)02718-1. Identification of novel cellular proteins that bind to the LC8 dynein light chain using a pepscan technique

Identification of novel cellular proteins that bind to the LC8 dynein light chain using a pepscan technique

10.1016/s0014-5793(01)02718-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Dynein is a minus end‐directed microtubule motor that serves multiple cellular functions. We have performed a fine mapping of the 8 kDa dynein light chain (LC8) binding sites throughout the development of a library of consecutive synthetic dodecapeptides covering the amino acid sequences of the various proteins known to interact with this dynein member according to the yeast two hybrid system. Two different consensus sequences were identified: GIQVD present in nNOS, in DNA cytosine methyl transferase and also in GKAP, where it is present twice in the protein sequence. The other LC8 binding motif is KSTQT, present in Bim, dynein heavy chain, Kid‐1, protein 4 and also in swallow. Interestingly, this KSTQT motif is also present in several viruses known to associate with microtubules during retrograde transport from the plasma membrane to the nucleus during viral infection.

Bibliography

RodrÄ±Ìguez-Crespo, I., YÃ©lamos, B., Roncal, F., Albar, J. P., Ortiz de Montellano, P. R., & Gavilanes, F. (2001). Identification of novel cellular proteins that bind to the LC8 dynein light chain using a pepscan technique. FEBS Letters, 503(2â3), 135â141. Portico.

Authors 6

Ignacio Rodrı́guez-Crespo (first)
Belén Yélamos (additional)
Fernando Roncal (additional)
Juan Pablo Albar (additional)
Paul R. Ortiz de Montellano (additional)
Francisco Gavilanes (additional)

References 26 Referenced 88

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Dates

Type	When
Created	22 years, 10 months ago (Oct. 14, 2002, 11:56 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 8:12 a.m.)
Indexed	3 months, 1 week ago (May 22, 2025, 3:11 a.m.)
Issued	24 years ago (Aug. 14, 2001)
Published	24 years ago (Aug. 14, 2001)
Published Online	24 years ago (Aug. 14, 2001)
Published Print	24 years ago (Aug. 17, 2001)

Funders 0

None

BibTeX

@article{Rodr_guez_Crespo_2001, title={Identification of novel cellular proteins that bind to the LC8 dynein light chain using a pepscan technique}, volume={503}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(01)02718-1}, DOI={10.1016/s0014-5793(01)02718-1}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Rodrı́guez-Crespo, Ignacio and Yélamos, Belén and Roncal, Fernando and Albar, Juan Pablo and Ortiz de Montellano, Paul R. and Gavilanes, Francisco}, year={2001}, month=aug, pages={135–141} }

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