DOI: 10.1016/s0014-5793(01)02509-1. Chlorophyll <i>b</i> is involved in long‐wavelength spectral properties of light‐harvesting complexes LHC I and LHC II

Chlorophyll b is involved in long‐wavelength spectral properties of light‐harvesting complexes LHC I and LHC II

10.1016/s0014-5793(01)02509-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Chlorophyll (Chl) molecules attached to plant light‐harvesting complexes (LHC) differ in their spectral behavior. While most Chl a and Chl b molecules give rise to absorption bands between 645 nm and 670 nm, some special Chls absorb at wavelengths longer than 700 nm. Among the Chl a/b‐antennae of higher plants these are found exclusively in LHC I. In order to assign this special spectral property to one chlorophyll species we reconstituted LHC of both photosystem I (Lhca4) and photosystem II (Lhcb1) with carotenoids and only Chl a or Chl b and analyzed the effect on pigment binding, absorption and fluorescence properties. In both LHCs the Chl‐binding sites of the omitted Chl species were occupied by the other species resulting in a constant total number of Chls in these complexes. 77‐K spectroscopic measurements demonstrated that omission of Chl b in refolded Lhca4 resulted in a loss of long‐wavelength absorption and 730‐nm fluorescence emission. In Lhcb1 with only Chl b long‐wavelength emission was preserved. These results clearly demonstrate the involvement of Chl b in establishing long‐wavelength properties.

Bibliography

Schmid, V. H. R., ThomÃ©, P., RÃ¼hle, W., Paulsen, H., KÃ¼hlbrandt, W., & Rogl, H. (2001). Chlorophyll b is involved in longâwavelength spectral properties of lightâharvesting complexes LHC I and LHC II. FEBS Letters, 499(1â2), 27â31. Portico.

Authors 6

Volkmar H.R. Schmid (first)
Peter Thomé (additional)
Wolfgang Rühle (additional)
Harald Paulsen (additional)
Werner Kühlbrandt (additional)
Hans Rogl (additional)

References 38 Referenced 34

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:51 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:13 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 6, 2025, 9:06 a.m.)
Issued	24 years, 2 months ago (June 14, 2001)
Published	24 years, 2 months ago (June 14, 2001)
Published Online	24 years, 2 months ago (June 14, 2001)
Published Print	24 years, 2 months ago (June 15, 2001)

Funders 0

None

BibTeX

@article{Schmid_2001, title={Chlorophyll b is involved in long‐wavelength spectral properties of light‐harvesting complexes LHC I and LHC II}, volume={499}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(01)02509-1}, DOI={10.1016/s0014-5793(01)02509-1}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Schmid, Volkmar H.R. and Thomé, Peter and Rühle, Wolfgang and Paulsen, Harald and Kühlbrandt, Werner and Rogl, Hans}, year={2001}, month=jun, pages={27–31} }

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