DOI: 10.1016/s0014-5793(01)02310-9. Substitution of a conserved aspartate allows cation‐induced polymerization of FtsZ

Substitution of a conserved aspartate allows cation‐induced polymerization of FtsZ

10.1016/s0014-5793(01)02310-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The prokaryotic tubulin homologue FtsZ polymerizes in vitro in a nucleotide dependent fashion. Here we report that replacement of the strictly conserved Asp212 residue of Escherichia coli FtsZ by a Cys or Asn, but not by a Glu residue results in FtsZ that polymerizes with divalent cations in the absence of added GTP. FtsZ D212C and D212N mutants co‐purify with GTP as bound nucleotide, providing an explanation for the unusual phenotype. We conclude that D212 plays a critical role in the coordination of a metal ion and the nucleotide at the interface of two FtsZ monomers.

Bibliography

Scheffers, D.-J., de Wit, J. G., den Blaauwen, T., & Driessen, A. J. M. (2001). Substitution of a conserved aspartate allows cationâinduced polymerization of FtsZ. FEBS Letters, 494(1â2), 34â37. Portico.

Authors 4

Dirk-Jan Scheffers (first)
Janny G de Wit (additional)
Tanneke den Blaauwen (additional)
Arnold J.M Driessen (additional)

References 33 Referenced 27

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 5:49 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 3:54 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 13, 2024, 12:44 p.m.)
Issued	24 years, 4 months ago (April 4, 2001)
Published	24 years, 4 months ago (April 4, 2001)
Published Online	24 years, 4 months ago (April 4, 2001)
Published Print	24 years, 4 months ago (April 6, 2001)

Funders 0

None

BibTeX

@article{Scheffers_2001, title={Substitution of a conserved aspartate allows cation‐induced polymerization of FtsZ}, volume={494}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(01)02310-9}, DOI={10.1016/s0014-5793(01)02310-9}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Scheffers, Dirk-Jan and de Wit, Janny G and den Blaauwen, Tanneke and Driessen, Arnold J.M}, year={2001}, month=apr, pages={34–37} }

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