DOI: 10.1016/s0014-5793(00)02245-6. Two novel calcium‐binding proteins from cytoplasmic granules of the protozoan parasite <i>Entamoeba histolytica</i>

Two novel calcium‐binding proteins from cytoplasmic granules of the protozoan parasite Entamoeba histolytica

10.1016/s0014-5793(00)02245-6

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

We report on the molecular characterisation of two novel granule proteins of the protozoon and human pathogen Entamoeba histolytica. The proteins, which were named grainin 1 and 2, show a considerable structural similarity to calcium‐binding proteins, particularly within EF‐hand motifs. Each grainin possesses three of these putative calcium‐binding sites. Based on careful inspection of known structures of protein families containing EF‐hands, a domain of grainin 1 covering two EF‐hand motifs was modeled by homology. Calcium‐binding activity of grainins was demonstrated by two independent methods. These granule proteins may be implicated in functions vital for the primitive phagocyte and destructive parasite such as control of endocytotic pathways and granule discharge.

Bibliography

Nickel, R., Jacobs, T., Urban, B., Scholze, H., Bruhn, H., & Leippe, M. (2000). Two novel calciumâbinding proteins from cytoplasmic granules of the protozoan parasite Entamoeba histolytica. FEBS Letters, 486(2), 112â116. Portico.

Authors 6

Rosa Nickel (first)
Thomas Jacobs (additional)
Britta Urban (additional)
Henning Scholze (additional)
Heike Bruhn (additional)
Matthias Leippe (additional)

References 28 Referenced 31

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{'key': 'e_1_2_5_29_1', 'first-page': '175', 'volume': '1', 'author': 'Hooman H.R.', 'year': '1999', 'journal-title': 'J. Mol. Microbiol. Biotechnol.'} / J. Mol. Microbiol. Biotechnol. by Hooman H.R. (1999)

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 4:59 p.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 9:07 p.m.)
Indexed	1 year, 1 month ago (July 12, 2024, 9:48 a.m.)
Issued	24 years, 8 months ago (Dec. 7, 2000)
Published	24 years, 8 months ago (Dec. 7, 2000)
Published Online	24 years, 8 months ago (Dec. 7, 2000)
Published Print	24 years, 8 months ago (Dec. 8, 2000)

Funders 0

None

BibTeX

@article{Nickel_2000, title={Two novel calcium‐binding proteins from cytoplasmic granules of the protozoan parasite Entamoeba histolytica}, volume={486}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(00)02245-6}, DOI={10.1016/s0014-5793(00)02245-6}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Nickel, Rosa and Jacobs, Thomas and Urban, Britta and Scholze, Henning and Bruhn, Heike and Leippe, Matthias}, year={2000}, month=dec, pages={112–116} }

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